In vivo measurement of human dermis by 1064 nm‐excited fiber Raman spectroscopy

Background/aims: Although chemical information on the dermis in vivo is highly important in skin research, an efficient method for gathering this information is yet to be developed. Here, we demonstrate that newly developed near‐infrared (1064 nm) excited Raman spectroscopy is a powerful method for chemical analysis of human skin in vivo.

[1]  Hiro-o Hamaguchi,et al.  1064 nm near‐infrared multichannel Raman spectroscopy of fresh human lung tissues , 2005 .

[2]  P. Delaney,et al.  View of normal human skin in vivo as observed using fluorescent fiber-optic confocal microscopic imaging. , 2003, The Journal of investigative dermatology.

[3]  H. Bruining,et al.  In vivo confocal Raman microspectroscopy of the skin: noninvasive determination of molecular concentration profiles. , 2001, The Journal of investigative dermatology.

[4]  D. A. Christopher,et al.  Advances in ultrasound biomicroscopy. , 2000, Ultrasound in medicine & biology.

[5]  H. Wulf,et al.  Water and protein structure in photoaged and chronically aged skin. , 1998, The Journal of investigative dermatology.

[6]  M. Manfait,et al.  An in vivo Randomized Study of Human Skin Moisturization by a New Confocal Raman Fiber-Optic Microprobe: Assessment of a Glycerol-Based Hydration Cream , 2006, Skin Pharmacology and Physiology.

[7]  J. Chung,et al.  Ultraviolet modulation of human macrophage metalloelastase in human skin in vivo. , 2002, The Journal of investigative dermatology.

[8]  B. Querleux,et al.  Lymphedematous skin and subcutis: in vivo high resolution magnetic resonance imaging evaluation. , 1998, The Journal of investigative dermatology.

[9]  Jean-Luc Leveque,et al.  Near-infrared spectroscopy: A new approach to the characterization of dry skin , 1993 .

[10]  I. Riemann,et al.  In vivo Drug Screening in Human Skin Using Femtosecond Laser Multiphoton Tomography , 2006, Skin Pharmacology and Physiology.

[11]  Takeshi Yasui,et al.  Determination of collagen fiber orientation in human tissue by use of polarization measurement of molecular second-harmonic-generation light. , 2004, Applied optics.

[12]  Young-Ah Woo,et al.  Determination of water content in skin by using a ft near infrared spectrometer , 2005, Archives of pharmacal research.

[13]  Robert Langer,et al.  Visualization of oleic acid-induced transdermal diffusion pathways using two-photon fluorescence microscopy. , 2003, The Journal of investigative dermatology.

[14]  G. Puppels,et al.  Combined in vivo confocal Raman spectroscopy and confocal microscopy of human skin. , 2003, Biophysical journal.

[15]  Thilo Gambichler,et al.  Acute skin alterations following ultraviolet radiation investigated by optical coherence tomography and histology , 2005, Archives of Dermatological Research.

[16]  J. Karras,et al.  Dermal delivery of topically applied oligonucleotides via follicular transport in mouse skin. , 2005, The Journal of investigative dermatology.

[17]  J. Welzel Optical coherence tomography in dermatology: a review , 2001, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[18]  J. Welzel,et al.  Influence of body water distribution on skin thickness: measurements using high‐frequency ultrasound , 2001, The British journal of dermatology.

[19]  E Panzig,et al.  In vivo and in vitro evidence for hydrogen peroxide (H2O2) accumulation in the epidermis of patients with vitiligo and its successful removal by a UVB-activated pseudocatalase. , 1999, The journal of investigative dermatology. Symposium proceedings.

[20]  C Edwards,et al.  Degenerative alterations of dermal collagen fiber bundles in photodamaged human skin and UV-irradiated hairless mouse skin: possible effect on decreasing skin mechanical properties and appearance of wrinkles. , 2001, The Journal of investigative dermatology.

[21]  P. Agache Stratum Corneum Histophysiology , 2004 .

[22]  O Jolivet,et al.  In vivo hydration profile in skin layers by high-resolution magnetic resonance imaging. , 1994, Skin pharmacology : the official journal of the Skin Pharmacology Society.

[23]  D. Thiboutot Regulation of human sebaceous glands. , 2004, The Journal of investigative dermatology.

[24]  James Varani,et al.  Collagen degradation in aged/photodamaged skin in vivo and after exposure to matrix metalloproteinase-1 in vitro. , 2003, The Journal of investigative dermatology.

[25]  J. Strauss The Sebaceous Glands , 1974 .

[26]  J. Voorhees,et al.  Reduced fibroblast interaction with intact collagen as a mechanism for depressed collagen synthesis in photodamaged skin. , 2004, The Journal of investigative dermatology.

[27]  R. Neubert,et al.  Noninvasive characterization of human stratum corneum of undiseased skin of patients with atopic dermatitis and psoriasis as studied by Fourier transform Raman spectroscopy. , 2001, Biopolymers.

[28]  L. Vaillant,et al.  Does hormonal skin aging exist? A study of the influence of different hormone therapy regimens on the skin of postmenopausal women using non-invasive measurement techniques. , 1996, Dermatology.

[29]  O Jolivet,et al.  Characterization of the skin in vivo by high resolution magnetic resonance imaging: water behavior and age-related effects. , 1993, The Journal of investigative dermatology.

[30]  Chen-Yuan Dong,et al.  Evaluating cutaneous photoaging by use of multiphoton fluorescence and second-harmonic generation microscopy. , 2005, Optics letters.