Virus removal by coagulation with polyelectrolytes.

1. Bacteriophage T2 of Esch. coli strain B at a concentration of 106-107 plaque forming units (PFU) per ml. 2. Type 1 poliovirus (Sabin) at a concentration of 3-7 X 104 PFU/ml. Procedures for the production and plaque assay of the bacteriophage were as described by Thayer and Sproul.2 The poliovirus was grown and harvested in LLC-MK2 (Hull) continuous monkey kidney cells.* The concentration and purification procedure was previously described by Wentworth et al* The plaque assays were done using LLC-MK2 cells and the procedure of Nakano and Gelfand.4

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[6]  H. Gelfand,et al.  The use of a modified Wecker technique for the serodifferentiation of type 1 polioviruses related and unrelated to Sabin's vaccine strain. I. Standardization and evaluation of the test. , 1962, American journal of hygiene.

[7]  G. Berg.  Virus transmission by the water vehicle. 3. Removal of viruses by water treatment procedures. , 1966, Health laboratory science.

[8]  R. Woodward,et al.  Removal of Coxsackie and bacterial viruses in water by flocculation. I. Removal of Coxsackie and bacterial viruses in water of known chemical content by flocculation with aluminum sulfate or ferric chloride under various testing conditions. , 1958, American journal of public health and the nation's health.

[9]  S. A. Hannah,et al.  Control Techniques for Coagulation‐Filtration , 1967 .