Low-pass filtering compensation in common-path digital holographic microscopy

A low-pass filtering compensation (LPFC) method is proposed to compensate for phase aberrations in point diffraction-based common-path digital holographic microscopy. This method estimates the phase aberration from the object hologram by Fourier transform and low-pass spatial filtering. The estimated phase aberration is subtracted from the object phase image to achieve single-hologram phase compensation. The accuracy and capability of LPFC for phase compensation were demonstrated by experiments on a Ronchi grating and a human blood smear. LPFC provides phase compensation for both smooth objects and objects containing abrupt edges, in the special case of a system with relatively high-frequency objects and low-frequency slight phase aberrations. LPFC operates without the need for fitting procedures, iterative steps, or prior knowledge of the optical parameters, which substantially simplifies the process of phase compensation in quantitative phase imaging.

[1]  E. Cuche,et al.  Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. , 2005, Optics letters.

[2]  Pietro Ferraro,et al.  Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging. , 2003, Applied optics.

[3]  Jianlin Zhao,et al.  Automatic compensation of phase aberrations in digital holographic microscopy based on sparse optimization , 2019, APL Photonics.

[4]  R. Dasari,et al.  Diffraction phase microscopy for quantifying cell structure and dynamics. , 2006, Optics letters.

[5]  Bahram Javidi,et al.  Stable and simple quantitative phase-contrast imaging by Fresnel biprism , 2018 .

[6]  Amir Arbabi,et al.  Detecting 20 nm wide defects in large area nanopatterns using optical interferometric microscopy. , 2013, Nano letters.

[7]  P. Ferraro,et al.  Direct full compensation of the aberrations in quantitative phase microscopy of thin objects by a single digital hologram , 2007 .

[8]  Feng Pan,et al.  Adaptive frequency filtering based on convolutional neural networks in off-axis digital holographic microscopy. , 2019, Biomedical optics express.

[9]  Christian Depeursinge,et al.  Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram. , 2006, Optics express.

[10]  Chun-Min Lo,et al.  High-resolution quantitative phase-contrast microscopy by digital holography. , 2005, Optics express.

[11]  Masoomeh Dashtdar,et al.  Quantitative phase imaging based on Fresnel diffraction from a phase plate , 2018, Applied Physics Letters.

[12]  Li Xiaoping,et al.  Automatic removal of phase aberration in holographic microscopy for drug sensitivity detection of ovarian cancer cells , 2020 .

[13]  Serge Monneret,et al.  Optical imaging and characterization of graphene and other 2D materials using quantitative phase microscopy , 2017 .

[14]  Qiusheng Lian,et al.  Automatic phase aberration compensation for digital holographic microscopy based on phase variation minimization. , 2018, Optics letters.

[15]  Etienne Cuche,et al.  Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation. , 2006, Applied optics.

[16]  Natan T Shaked,et al.  Quantitative phase microscopy of biological samples using a portable interferometer. , 2012, Optics letters.

[17]  Baoli Yao,et al.  Parallel two-step phase-shifting point-diffraction interferometry for microscopy based on a pair of cube beamsplitters. , 2011, Optics express.

[18]  Pinhas Girshovitz,et al.  Doubling the field of view in off-axis low-coherence interferometric imaging , 2014, Light: Science & Applications.

[19]  Bahram Javidi,et al.  Common-path, single-shot phase-shifting digital holographic microscopy using a Ronchi ruling , 2019, Applied Physics Letters.

[20]  Pasquale Memmolo,et al.  Tomographic flow cytometry by digital holography , 2016, Light: Science & Applications.

[21]  George Nehmetallah,et al.  Accurate quantitative phase digital holographic microscopy with single- and multiple-wavelength telecentric and nontelecentric configurations. , 2016, Applied optics.

[22]  A Finizio,et al.  Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction. , 2010, Optics letters.