MiR-222 promotes proliferation, migration and invasion of lung adenocarcinoma cells by targeting ETS1.

OBJECTIVE The lung adenocarcinoma is a type of lung cancer. This research is to investigate the effects of miR-222 on the proliferation, migration and invasion of the lung adenocarcinoma cells. MATERIALS AND METHODS At the beginning, MiR-222 and the controls were transfected to the lung adenocarcinoma cell line A549 for CCK-8 proliferation, transwell migration and Matrigel invasion, and then observed the effect of miR-222 on the proliferation, migration and invasion of lung adenocarcinoma cells. The miR-222 target was regulated by ETS1 downwards to participate in the regulation of the process by using the luciferase reporter assay, the Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and the Western blotting. RESULTS According to CCK-8 proliferation assay, the Transwell migration and the Matrigel invasion assay, it discovered that MiR-222 can promote the proliferation, migration and invasion of the lung adenocarcinoma cells. Luciferase reporter assay, RT-qPCR and Western blot assay showed that miR-222 could regulate the expression of ETS1 downwards and ETS1 participated in the regulation of the process CONCLUSIONS: ETS1 promotes proliferation, migration and invasion of lung adenocarcinoma cells by targeting the regulated miR-222 downwards.

[1]  D. Dornan,et al.  miR-221/222 Targets Adiponectin Receptor 1 to Promote the Epithelial-to-Mesenchymal Transition in Breast Cancer , 2013, PloS one.

[2]  Hui-huang Lin,et al.  4-Nitroquinoline-1-oxide effects human lung adenocarcinoma A549 cells by regulating the expression of POLD4. , 2016, Biomedical reports.

[3]  M. Wang,et al.  Bioinformatics analysis of two microarray gene-expression data sets to select lung adenocarcinoma marker genes. , 2012, European review for medical and pharmacological sciences.

[4]  R. Govindan,et al.  Genomic alterations in lung adenocarcinoma. , 2015, The Lancet. Oncology.

[5]  L. O’Driscoll,et al.  Global analysis of serum microRNAs as potential biomarkers for lung adenocarcinoma , 2013, Cancer biology & therapy.

[6]  K. Syrigos,et al.  The VEGF pathway in lung cancer , 2013, Cancer Chemotherapy and Pharmacology.

[7]  X. Zhao,et al.  MiR-221 and miR-222 simultaneously target ARID1A and enhance proliferation and invasion of cervical cancer cells. , 2016, European review for medical and pharmacological sciences.

[8]  J. Dittmer.  The role of the transcription factor Ets1 in carcinoma. , 2015, Seminars in cancer biology.

[9]  P. Pu,et al.  MicroRNA-221 and microRNA-222 regulate gastric carcinoma cell proliferation and radioresistance by targeting PTEN , 2010, BMC Cancer.

[10]  Shengguang Ding,et al.  MicroRNA-222 promotes human non-small cell lung cancer H460 growth by targeting p27. , 2015, International journal of clinical and experimental medicine.

[11]  A. Jemal,et al.  Cancer statistics, 2014 , 2014, CA: a cancer journal for clinicians.

[12]  Alessandra Carè,et al.  Constitutive activation of the ETS-1-miR-222 circuitry in metastatic melanoma , 2011, Pigment cell & melanoma research.

[13]  Yang Di,et al.  Elevated expression of tumor miR-222 in pancreatic cancer is associated with Ki67 and poor prognosis , 2013, Medical Oncology.

[14]  Carlos Cordon-Cardo,et al.  miR-143, miR-222, and miR-452 are useful as tumor stratification and noninvasive diagnostic biomarkers for bladder cancer. , 2012, The American journal of pathology.

[15]  M. Mino‐Kenudson,et al.  Tumor islands and spread through air spaces: Distinct patterns of invasion in lung adenocarcinoma , 2016, Pathology international.

[16]  F. McCormick,et al.  EGFR inhibition evokes innate drug resistance in lung cancer cells by preventing Akt activity and thus inactivating Ets-1 function , 2015, Proceedings of the National Academy of Sciences.

[17]  Hyojin Kim,et al.  Overview of clinicopathologic features of ALK-rearranged lung adenocarcinoma and current diagnostic testing for ALK rearrangement. , 2015, Translational lung cancer research.

[18]  N. Raghavachari,et al.  miR-222 contributes to sex-dimorphic cardiac eNOS expression via ets-1. , 2013, Physiological genomics.