Production of volatile compounds by hairy root cultures of Cichorium intybus L. under the influence of fungal elicitors and their analysis using solid-phase micro extraction gas chromatography-mass spectrometry

Hairy root cultures of Cichorium intybus L produced volatile aromatic compounds under the influence of fungal elicitors. It was observed that the intensity of the production of volatile aromatic compounds in the hairy root cultures of C intybus with 10 ml l−1 media filtrate (MF) of Phytopthora parasitica var nicotiana reached a maximum on the 21st day, as seen by a quantitative flavour profiling method. It was noted that, during the time course, treatment with 10 ml l−1 MF of P parasitica to the MS basal liquid medium containing hairy root cultures of chicory resulted in changed root morphology with cell wall thickening and shear. The components of the volatile aromatic compounds were identified as a propyl isovalerate, undecanal, nonanol, isoamyl nonanoate and 2-decene-1-ol. The mass spectra of all these compounds matched well with the NIST/EPA/NIH library and by comparing with the Kovats index of volatile compounds. The major fruity note was due to the presence of two of the major volatile components, namely propyl isovalerate and isoamyl nonanoate, which were also produced at higher concentration on day 21. This response of chicory hairy root cultures to the fungal elicitor producing volatile aromatic compounds would imply eco-physiological functions, possibly for plant defence system. Copyright © 2003 Society of Chemical Industry

[1]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .

[2]  F. Dicosmo,et al.  Eliciting secondary metabolism in plant cell cultures , 1985 .

[3]  J. W. Arnold,et al.  Use of digital aroma technology and SPME GC-MS to compare volatile compounds produced by bacteria isolated from processed poultry† , 1998 .

[4]  S. Rao,et al.  Biotransformation of isoeugenol to vanilla flavour metabolites and capsaicin in suspended and immobilized cell cultures of Capsicum frutescens: study of the influence of β-cyclodextrin and fungal elicitor , 1999 .

[5]  T. Peppard,et al.  Solid-Phase Microextraction for Flavor Analysis , 1994 .

[6]  Gokare A. Ravishankar,et al.  Biotransformation of ferulic acid and vanillylamine to capsaicin and vanillin in immobilized cell cultures of Capsicum frutescens , 1996, Plant Cell Tissue and Organ Culture.

[7]  C. Sanz,et al.  Aroma components and free amino acids in strawberry variety Chandler during ripening , 1992 .

[8]  J. Vivanco,et al.  Root-specific metabolism: The biology and biochemistry of underground organs , 2001, In Vitro Cellular & Developmental Biology - Plant.

[9]  J. Oakey,et al.  Interaction of methyl jasmonate, wounding and fungal elicitation during sesquiterpene induction in Hyoscyamus muticus in root cultures , 1998, Plant Cell Reports.

[10]  H. Bais,et al.  Cichorium intybus L – cultivation, processing, utility, value addition and biotechnology, with an emphasis on current status and future prospects , 2001 .

[11]  N. Fischer,et al.  Gas Chromatography—Olfactometry as a Tool for Measuring Flavor—Food Ingredient Interactions in Model Systems , 1996 .

[12]  Gokare A. Ravishankar,et al.  Flavour production in plant cell cultures of basmati rice (Oryza sativa L) , 1994 .

[13]  Gokare A. Ravishankar,et al.  Induction of crocin, crocetin, picrocrocin, and safranal synthesis in callus cultures of saffron: Crocus sativus L , 1990 .

[14]  Gokare A. Ravishankar,et al.  Food applications of plant cell cultures. , 1990 .

[15]  I. Galpin,et al.  COMPARISON OF THE ONION PLANT (ALLIUM CEPA) AND ONION TISSUE CULTURE IV. EFFECT OF SHOOT AND ROOT MORPHOGENESIS ON FLAVOUR PRECURSOR SYNTHESIS IN ONION TISSUE CULTURE , 1981 .

[16]  M. McDaniel,et al.  Odor analysis of pinot noir wines from grapes of different maturities by a gas chromatography-olfactometry technique (osme) , 1992 .

[17]  H. Bais,et al.  Enhancement of growth and coumarin production in hairy root cultures of witloof chicory (Cichorium intybus L.cv. Lucknow local) under the influence of fungal elicitors. , 2000, Journal of bioscience and bioengineering.

[18]  Jun Song,et al.  Application of Solid Phase Microextraction and Gas Chromatography/Time-of-Flight Mass Spectrometry for Rapid Analysis of Flavor Volatiles in Tomato and Strawberry Fruits , 1998 .

[19]  J. Pawliszyn,et al.  Solid phase microextraction with thermal desorption using fused silica optical fibers , 1990 .

[20]  R. Berger,et al.  Elicitation of volatile compounds in photomixotrophic cell culture of Petroselinum crispum , 1996, Plant Cell, Tissue and Organ Culture.

[21]  Jorge Ruiz,et al.  Headspace solid phase microextraction for the analysis of volatiles in a meat product : dry-cured iberian ham , 1998 .

[22]  A. Baerheim Svendsen,et al.  Production of essential oils and flavours in plant cell and tissue cultures. A review , 2004, Plant Cell, Tissue and Organ Culture.