Electrochemical determination of disulfoton using a molecularly imprinted poly-phenol polymer

A simple, rapid and sensitive electrochemical method using a molecularly 13 imprinted poly-phenol polymer for the analysis of disulfoton in model and real samples is 14 demonstrated. A computational approach to molecularly imprinted polymer design and 15 screening is followed using density functional (B3LYP) and Semi-Empirical Parameterized 16 Model number 3 (PM3) models. The selected phenol monomer is electrochemically 17 polymerized by cyclic voltammetry at a glassy carbon working electrode in the presence of a 18 disulfoton template. The subsequent molecularly imprinted polymer sensor exhibits an 19 oxidation peak at 1.13 V vs. Ag/AgCl in cyclic voltammetry with excellent linearity 20 (r2=0.9985) over the range 1-30 μM. The limit of detection for the DSN-MIP is 0.183 μM, 21 compared to a limit of detection of 1.64 μM with cyclic voltammetry for the bare glassy 22 carbon electrode. Intra- and inter-day assay precisions, expressed as relative standard 23 deviation, are both found to be less than 7% overall. The developed molecularly imprinted 24 polymer sensor is utilized to determine disulfoton in both spiked synthetic human plasma 25 and human urine samples with recoveries ranging from 85.2% to 101.1%. The developed 26 methods can be applied for measuring this toxicant in a real sample.

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