Diminution of oxalate induced renal tubular epithelial cell injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribulus terrestris.

PURPOSE Recurrence and persistent side effects of present day treatment for urolithiasis restrict their use, so an alternate solution, using phytotherapy is being sought. The present study attempted to evaluate the antilithiatic properties of Tribulus terrestris commonly called as "gokhru" which is often used in ayurveda to treat various urinary diseases including urolithiasis. MATERIALS AND METHODS The activity of Tribulus terrestris was investigated on nucleation and the growth of the calcium oxalate (CaOx) crystals as well as on oxalate induced cell injury of NRK 52E renal epithelial cells. RESULTS Tribulus terrestris extract exhibited a concentration dependent inhibition of nucleation and the growth of CaOx crystals. When NRK-52E cells were injured by exposure to oxalate for 72 h, Tribulus terrestris extract prevented the injury in a dose-dependent manner. On treatment with the different concentrations of the plant, the cell viability increased and lactate dehydrogenase release decreased in a concentration dependent manner. CONCLUSION The current data suggests that Tribulus terrestris extract not only has a potential to inhibit nucleation and the growth of the CaOx crystals but also has a cytoprotective role. Our results indicate that it could be a potential candidate for phytotherapy against urolithiasis.

[1]  M. Monga,et al.  Use of traditional Chinese medicine in the management of urinary stone disease. , 2009, International braz j urol : official journal of the Brazilian Society of Urology.

[2]  C. Tandon,et al.  A Novel Calcium Oxalate Crystal Growth Inhibitory Protein from the Seeds of Dolichos biflorus (L.) , 2009, The protein journal.

[3]  A. Gilani,et al.  Antiurolithic effect of Bergenia ligulata rhizome: an explanation of the underlying mechanisms. , 2009, Journal of ethnopharmacology.

[4]  C. Tandon,et al.  Purification and characterization of an anticalcifying protein from the seeds of Trachyspermum ammi (L.). , 2009, Protein and peptide letters.

[5]  M. Monga,et al.  Ayurvedic medicine and renal calculi. , 2008, Journal of endourology.

[6]  S. Ghalem,et al.  Inhibition of calcium oxalate monohydrate crystal growth using Algerian medicinal plants , 2008 .

[7]  K. Miyazawa,et al.  Reduction in oxalate-induced renal tubular epithelial cell injury by an extract from Quercus salicina Blume/Quercus stenophylla Makino , 2007, Urological Research.

[8]  A. Verhulst,et al.  Proposed mechanisms in renal tubular crystal retention. , 2007, Kidney international.

[9]  D. Sujatha,et al.  Herbal Drugs in Urolithiasis - A Review , 2007 .

[10]  L. P. Mercuri,et al.  Effect of extract of Phyllanthus niruri on crystal deposition in experimental urolithiasis , 2006, Urological Research.

[11]  J. Jonassen,et al.  Oxalate toxicity in renal cells , 2005, Urological Research.

[12]  P. Chandhoke,et al.  Oxalate toxicity in cultured mouse inner medullary collecting duct cells. , 2005, The Journal of urology.

[13]  A. Vaidya,et al.  Herbal extracts of Tribulus terrestris and Bergenia ligulata inhibit growth of calcium oxalate monohydrate crystals in vitro , 2005 .

[14]  M. Daudon,et al.  A new approach to studying inhibitors of calcium oxalate crystal growth , 1993, Urological Research.

[15]  C. Kwak,et al.  Apoptosis induced by oxalate in human renal tubular epithelial HK-2 cells , 2005, Urological Research.

[16]  J. Lieske,et al.  Extract from Herniaria hirsuta coats calcium oxalate monohydrate crystals and blocks their adhesion to renal epithelial cells. , 2004, The Journal of urology.

[17]  Saeed R. Khan,et al.  Calcium oxalate crystal interaction with renal tubular epithelium, mechanism of crystal adhesion and its impact on stone development , 2004, Urological Research.

[18]  J. Lieske,et al.  Annexin II is present on renal epithelial cells and binds calcium oxalate monohydrate crystals. , 2003, Journal of the American Society of Nephrology : JASN.

[19]  M. E. Barros,et al.  Effects of an aqueous extract from Phyllantus niruri on calcium oxalate crystallization in vitro , 2003, Urological Research.

[20]  N. Schor,et al.  Phyllanthus niruri Inhibits Calcium Oxalate Endocytosis by Renal Tubular Cells: Its Role in Urolithiasis , 1999, Nephron.

[21]  E. Worcester,et al.  Control of calcium oxalate crystal structure and cell adherence by urinary macromolecules. , 1998, Kidney international.

[22]  F. Schröder,et al.  Association of calcium oxalate monohydrate crystals with MDCK cells. , 1995, Kidney international.

[23]  D. Kok,et al.  Calcium oxalate nephrolithiasis, a free or fixed particle disease. , 1994, Kidney international.

[24]  B. Dhawan,et al.  Activity of certain fractions of Tribulus terrestris fruits against experimentally induced urolithiasis in rats. , 1994, Indian journal of experimental biology.

[25]  W. Amer,et al.  Extracts and fractions of Thymus capitatus exhibit antimicrobial activities. , 1994, Journal of ethnopharmacology.

[26]  S. Srivastava,et al.  Evaluation of Antiurolithiatic Activity of Tribulus Terrestris , 1994 .

[27]  J. Engasser,et al.  The use of lactate dehydrogenase (LDH) release kinetics for the evaluation of death and growth of mammalian cells in perfusion reactors , 1992, Biotechnology and bioengineering.

[28]  F. Coe,et al.  Urine glycoprotein crystal growth inhibitors. Evidence for a molecular abnormality in calcium oxalate nephrolithiasis. , 1985, The Journal of clinical investigation.

[29]  J. Parks,et al.  Evidence for a Molecular Abnormality in Calcium Oxalate Nephrolithiasis , 1985 .

[30]  R. Sahota,et al.  Effect of the aqueous extract of an Ayurvedic compound preparation on mineralization & demineralization reactions. , 1983, The Indian journal of medical research.