Discrepancies and Interpretation Problems in Susceptibility Testing of VIM-1-Producing Klebsiella pneumoniae Isolates

ABSTRACT Susceptibilities to β-lactam antibiotics of five VIM-1-producing Klebsiella pneumoniae isolates were determined by broth microdilution, Etest, disk diffusion, and the automated systems Vitek 2, Phoenix, and MicroScan. Significant discrepancies were observed in the determination of susceptibility to imipenem and meropenem. Interpretation problems by the automated systems were also noted.

[1]  V. Miriagou,et al.  Escherichia coli with a Self-Transferable, Multiresistant Plasmid Coding for Metallo-β-Lactamase VIM-1 , 2003, Antimicrobial Agents and Chemotherapy.

[2]  Mary Jane Ferraro,et al.  Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically : approved standard , 2000 .

[3]  Clinical,et al.  Performance standards for antimicrobial disk susceptibility tests : approved standard , 2006 .

[4]  J. Wu,et al.  Metallo-beta-lactamase-producing Enterobacteriaceae isolates in a university hospital in Taiwan: prevalence of IMP-8 in Enterobacter cloacae and first identification of VIM-2 in Citrobacter freundii. , 2002, The Journal of antimicrobial chemotherapy.

[5]  Y. Tselentis,et al.  Spread of blaVIM-1-producing e. coli in a university hospital in Greece. Genetic analysis of the integron carrying the blaVIM-1 metallo-β-lactamase gene , 2004 .

[6]  R N Jones,et al.  Multicenter laboratory evaluation of the bioMérieux Vitek antimicrobial susceptibility testing system with 11 antimicrobial agents versus members of the family Enterobacteriaceae and Pseudomonas aeruginosa , 1997, Journal of clinical microbiology.

[7]  Yehuda Carmeli,et al.  Failure of Quality Control Measures To Prevent Reporting of False Resistance to Imipenem, Resulting in a Pseudo-Outbreak of Imipenem-Resistant Pseudomonas aeruginosa , 1998, Journal of Clinical Microbiology.

[8]  D. Yong,et al.  A new integron carrying VIM-2 metallo-beta-lactamase gene cassette in a Serratia marcescens isolate. , 2002, Diagnostic microbiology and infectious disease.

[9]  J. Waitz.  Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically , 1990 .

[10]  V. Miriagou,et al.  VIM-1 Metallo-β-Lactamase-Producing Klebsiella pneumoniae Strains in Greek Hospitals , 2003, Journal of Clinical Microbiology.

[11]  V. Miriagou,et al.  Imipenem Resistance in a Salmonella Clinical Strain Due to Plasmid-Mediated Class A Carbapenemase KPC-2 , 2003, Antimicrobial Agents and Chemotherapy.

[12]  S. Jeong,et al.  Characterization of a new integron containing VIM-2, a metallo- beta-lactamase gene cassette, in a clinical isolate of Enterobacter cloacae. , 2003, The Journal of antimicrobial chemotherapy.

[13]  P. Nordmann,et al.  Emerging carbapenemases in Gram-negative aerobes. , 2002, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[14]  R. Gaynes,et al.  Antimicrobial Susceptibility Testing of Carbapenems: Multicenter Validity Testing and Accuracy Levels of Five Antimicrobial Test Methods for Detecting Resistance in Enterobacteriaceae and Pseudomonas aeruginosa Isolates , 2003, Journal of Clinical Microbiology.

[15]  J. Frère,et al.  Purification and Biochemical Characterization of the VIM-1 Metallo-β-Lactamase , 2000, Antimicrobial Agents and Chemotherapy.