Determination of total content of coumarin in Angelica dahurica by UV spectrophotometry

OBJECTIVE To determine the total content of coumarin in Angelica dahurica. METHODS The Coumarin in Chuan Baizhi was detected at 300 nm by UV method.RESULTS There was a good linear relationship within the range of 2.2-11.0 μg·ml -1 (r=0.9999).The average recovery was 101.32%(n=6) with RSD of 3.34%. CONCLUSION This method is rapid, reproducible, accurate and can be used for measuring the total content of coumarin in Chuan Baizhi.