CURRENT LASER MICROIRRADIATION STUDIES *

The use of a laser in microbeam irradiation was first described by Bessis' in 1962. Since that time, there have been numerous publications and several reviews on the use of the laser for biological m i ~ r o i r r a d i a t i o n . ~ ~ Fundamentally, laser microbeam studies fall into two categories: (1) studies that essentially describe the effect(s) of a focused laser beam o n the cell, (2) studies that employ the laser microbeam as a tool t o approach functional biological questions. Both of the above are not mutually exclusive, and indeed there are studies in which a structural effect is described and a biological question is answered. For the large part, however, investigations have fallen separately into one or the other category. The general approach that we have been taking is t o study cell function by laser microirradiation of various subcellular structures. Investigations have fallen into both of the categories mentioned i n the previous paragraph, and they have been reviewed recently4 In this manuscript, we will briefly review past investigations and present data from some more recent experiments.

[1]  M. Berns.  Directed Chromosome Loss by Laser Microirradiation , 1974, Science.

[2]  M. Berns,et al.  Laser microdissection of actinomycin D segregated nucleoli. , 1974, Experimental cell research.

[3]  M. Berns,et al.  LIGHT AND ELECTRON MICROSCOPY OF LASER MICROIRRADIATED CHROMOSOMES , 1974, The Journal of cell biology.

[4]  M. Berns,et al.  The totipotency and relationship of seta-bearing cells to thallus development in the Green Alga Coleochaete Scutata. A laser microbeam study. , 1974, Developmental biology.

[5]  M. Berns,et al.  Partial Cell Irradiation with a Tunable Organic Dye Laser , 1972, Nature.

[6]  M W Berns,et al.  Laser microirradiation of the nucleolar organizer in cells of the rat kangaroo (Potorous tridactylis). Reduction of nucleolar number and production of micronucleoli. , 1972, Experimental cell research.

[7]  E. Chu,et al.  Induction of auxotrophic mutations by treatment of Chinese hamster cells with 5-bromodeoxyuridine and black light. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[8]  W. Dove,et al.  Photosensitization of transcription by bromodeoxyuridine substitution. , 1972, Journal of molecular biology.

[9]  M W Berns,et al.  Argon laser micro-irradiation of mitochondria in rat myocardial cells in tissue culture. II. Correlation of morphology and function in irradiated single cells. , 1972, Journal of molecular and cellular cardiology.

[10]  M. M. Nass,et al.  EFFECTS OF ETHIDIUM BROMIDE ON THE CYTOCHROME CONTENT AND ULTRASTRUCTURE OF L CELL MITOCHONDRIA , 1971, Journal of Cell Biology.

[11]  M W Berns,et al.  Are chromosome secondary constrictions nucleolar organizers? A re-examination using a laser microbeam. , 1971, Experimental cell research.

[12]  K. Kohn,et al.  Inhibition of the processing of ribosomal precursor RNA by intercalating agents. , 1971, Journal of molecular biology.

[13]  M. Berns.  A simple and versatile argon laser microbeam. , 1971, Experimental cell research.

[14]  M. Berns,et al.  Laser photosensitization and metabolic inhibition of tissue culture cells treated with quinacrine hydrochloride. , 1970, Life sciences. Pt. 2: Biochemistry, general and molecular biology.

[15]  S. Penman,et al.  Selective inhibition of the synthesis of mitochondria-associated RNA by ethidium bromide. , 1969, Journal of molecular biology.

[16]  M. Berns,et al.  Effects of laser micro-irradiation on chromosomes. , 1969, Experimental cell research.

[17]  J. Gall,et al.  Formation and detection of RNA-DNA hybrid molecules in cytological preparations. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[18]  M W Berns,et al.  LASER MICROBEAM STUDIES ON TISSUE CULTURE CELLS * , 1969, Annals of the New York Academy of Sciences.

[19]  R. Storb,et al.  Ruby laser micro-irradiation of single tissue culture cells vitally stained with Janus green B. II. Effects on dehydrogenase activities. , 1967, Experimental cell research.

[20]  G E Mark,et al.  Pacemaker activity and mitosis in cultures of newborn rat heart ventricle cells. , 1966, Experimental cell research.

[21]  M. Berns.  Laser microirradiation of chromosomes. , 1974, Cold Spring Harbor symposia on quantitative biology.

[22]  M. Berns.  Recent Progress with Laser Microbeams , 1974 .

[23]  M W Berns,et al.  Laser microbeams for partial cell irradiation. , 1972, International review of cytology.

[24]  L. Lerman.  The structure of the DNA-acridine complex. , 1963, Proceedings of the National Academy of Sciences of the United States of America.