Cylindrical micro touch sensor with a piezoelectric thin film for microbial separation

Isolation and separation of the target microbe or cell from a large heterogeneous population is quite important. We propose a new touch sensor that is used for the separation of the target microbe with a pipette. This sensor is sensitive enough to protect the fragile pipette tip from abrupt collision. Using this sensor, we developed a novel separation system for screening a target microorganisms from the randomly distributed samples in the dish with the local viscosity control of the thermosensitive hydrogel. With this system, the target yeast cell was extracted successfully.

[1]  W. Benecke,et al.  Linear motion of dielectric particles and living cells in microfabricated structures induced by traveling electric fields , 1991, [1991] Proceedings. IEEE Micro Electro Mechanical Systems.

[2]  Takashi Kaneko,et al.  A new smart vision system using a quick-response dynamic focusing lens , 2000, Proceedings IEEE Thirteenth Annual International Conference on Micro Electro Mechanical Systems (Cat. No.00CH36308).

[3]  G. Fuhr,et al.  Paired microelectrode system: dielectrophoretic particle sorting and force calibration , 1999 .

[4]  Fumihito Arai,et al.  3D bio-micromanipulation (bilateral control system using micro tri-axial force sensor) , 1999, MHS'99. Proceedings of 1999 International Symposium on Micromechatronics and Human Science (Cat. No.99TH8478).

[5]  Fumihito Arai,et al.  Microtouch-Sensor Array Fabricated by Hydrothermal Method , 2002, J. Robotics Mechatronics.

[6]  Fumihito Arai,et al.  Parallel beam micro sensor/actuator unit using PZT thin films and its application examples , 1998, Proceedings. 1998 IEEE International Conference on Robotics and Automation (Cat. No.98CH36146).

[7]  Fumihito Arai,et al.  Mechanical micro-dissection by microknife using ultrasonic vibration and ultra fine touch probe sensor , 2001, Proceedings 2001 ICRA. IEEE International Conference on Robotics and Automation (Cat. No.01CH37164).

[8]  Hannes Bleuler,et al.  Non-contact atomic force microscope with a PZT cantilever used for deflection sensing, direct oscillation and feedback actuation , 2002 .

[9]  Alan P. Morrison,et al.  Development of a microfluidic device for fluorescence activated cell sorting , 2002 .

[10]  Fumihito Arai,et al.  Fixation and Isolation of Microorganisms by Local Viscosity Control of Methyl Cellulose Solution , 2002 .

[11]  Fumihito Arai,et al.  Separation of Target Microbe by Laser Manipulation and Flow Control , 2002, J. Robotics Mechatronics.

[12]  Fumihito Arai,et al.  High‐speed separation system of randomly suspended single living cells by laser trap and dielectrophoresis , 2001, Electrophoresis.

[13]  S. M. Harb,et al.  Resonator-based touch-sensitive probe , 1995 .

[14]  Fumihito Arai,et al.  3D calibration for micro-manipulation with precise position measurement , 2001 .

[15]  T. Morita,et al.  A cylindrical micro ultrasonic motor using PZT thin film deposited by single process hydrothermal method (/spl phi/2.4 mm, L=10 mm stator transducer) , 1998, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[16]  T. Itoh,et al.  Self-excited piezoelectric PZT microcantilevers for dynamic SFM—with inherent sensing and actuating capabilities , 1999 .

[17]  M. Kurosawa,et al.  A rod-shaped vibro touch sensor using PZT thin film , 1998, Proceedings MEMS 98. IEEE. Eleventh Annual International Workshop on Micro Electro Mechanical Systems. An Investigation of Micro Structures, Sensors, Actuators, Machines and Systems (Cat. No.98CH36176.

[18]  Fumihito Arai,et al.  Micro touch sensor array made by hydrothermal method , 2001, MHS2001. Proceedings of 2001 International Symposium on Micromechatronics and Human Science (Cat. No.01TH8583).

[19]  Takaaki Tsurumi,et al.  Analysis of Bending Displacement of Lead Zirconate Titanate Thin Film Synthesized by Hydrothermal Method , 1993 .

[20]  Myron R. Melamed,et al.  Flow Cytometry and Sorting, Second Edition , 1991 .

[21]  Fumihito Arai,et al.  Isolation and extraction of target microbes using thermal sol-gel transformation. , 2003, The Analyst.

[22]  Fumihito Arai,et al.  High throughput separation of microorganisms by pinpoint gelation controlled by local heating for parallel incubation on a chip , 2003, The Sixteenth Annual International Conference on Micro Electro Mechanical Systems, 2003. MEMS-03 Kyoto. IEEE.

[23]  Pieter Telleman,et al.  Rare Event Cell Surging in a Microfluidic System for Application in Prenatal Diagnosis , 1998 .

[24]  T. Katsuragi,et al.  Single‐Cell Sorting of Microorganisms by Flow or Slide‐Based (Including Laser Scanning) Cytometry , 2001 .