Vaccine efficacy and immune response to Mycoplasma hyopneumoniae challenge in pigs vaccinated against porcine reproductive and respiratory syndrome virus and M hyopneumoniae

Methods: Pigs were vaccinated once with MLV PRRS vaccine (Day 0), twice with MH vaccine (Days 8 and 22), or both, and challenged with MH on Day 36. Necropsies were performed on Days 35 and 6163. Efficacy of MH vaccine was determined by reduction in the percentage of lung affected by mycoplasmal pneumonia. Serum antibodies to PRRS virus (PRRSV) and MH, antibodies to MH in bronchoalveolar lavage fluid (BAL), and production of MH-specific interferongamma(IFN-γ-) secreting cells in tissues and peripheral blood were measured on Days 0 and 14 and at necropsy. Immunological assays included ELISAs for PRRSV and MH antibodies and an enzyme-linked immunospot assay for MH-specific IFN-γsecreting cells.

[1]  E. Thacker,et al.  Evaluation of local and systemic immune responses induced by intramuscular injection of a Mycoplasma hyopneumoniae bacterin to pigs. , 2000, American journal of veterinary research.

[2]  P. Halbur,et al.  Effect of vaccination on the potentiation of porcine reproductive and respiratory syndrome virus (PRRSV)-induced pneumonia by Mycoplasma hyopneumoniae. , 2000, Vaccine.

[3]  P. Halbur,et al.  Mycoplasma hyopneumoniae Potentiation of Porcine Reproductive and Respiratory Syndrome Virus-Induced Pneumonia , 1999, Journal of Clinical Microbiology.

[4]  F. Zuckermann,et al.  Interleukin-12 enhances the virus-specific interferon gamma response of pigs to an inactivated pseudorabies virus vaccine. , 1998, Veterinary immunology and immunopathology.

[5]  E. Thacker,et al.  Comparison of antibody production, lymphocyte stimulation, and protection induced by four commercial Mycoplasma hyopneumoniae bacterins , 1998 .

[6]  E. Albina,et al.  Immune responses in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV) , 1998, Veterinary Immunology and Immunopathology.

[7]  S. Djordjevic,et al.  Serum and mucosal antibody responses and protection in pigs vaccinated against Mycoplasma hyopneumoniae with vaccines containing a denatured membrane antigen pool and adjuvant. , 1997, Australian veterinary journal.

[8]  T. Molitor,et al.  Cell-mediated immunity to porcine reproductive and respiratory syndrome virus in swine. , 1997, Viral immunology.

[9]  W. V. Van Alstine,et al.  Porcine reproductive and respiratory syndrome virus does not exacerbate Mycoplasma hyopneumoniae infection in young pigs. , 1996, Veterinary microbiology.

[10]  P. Halbur,et al.  Comparative Pathogenicity of Nine US Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Isolates in a Five-Week-Old Cesarean-Derived, Colostrum-Deprived Pig Model , 1996, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[11]  P. Halbur,et al.  Comparison of the Pathogenicity of Two US Porcine Reproductive and Respiratory Syndrome Virus Isolates with that of the Lelystad Virus , 1995, Veterinary pathology.

[12]  E. Thacker,et al.  In vitro analysis of a primary, major histocompatibility complex (MHC)-restricted, cytotoxic T-lymphocyte response to avian leukosis virus (ALV), using target cells expressing MHC class I cDNA inserted into a recombinant ALV vector , 1995, Journal of virology.

[13]  S. Messier,et al.  Interactions of Mycoplasma hyopneumoniae membranes with porcine lymphocytes. , 1991, American journal of veterinary research.

[14]  H. Joo,et al.  Evaluation of the ELISA and comparison to the complement fixation test and radial immunodiffusion enzyme assay for detection of antibodies against Mycoplasma hyopneumoniae in swine serum. , 1990, Veterinary microbiology.

[15]  Ross Rf,et al.  Characteristics of protective activity of Mycoplasma hyopneumoniae vaccine. , 1984 .

[16]  C. Weng,et al.  Diagnosis of mycoplasmal pneumonia of swine: sequential study by direct immunofluorescence. , 1984, American journal of veterinary research.

[17]  T. Young,et al.  Characteristics of protective activity of Mycoplasma hyopneumoniae vaccine. , 1984, American journal of veterinary research.

[18]  A. D. de Weck,et al.  Cell-mediated and humoral immune response in swine after vaccination and natural infection with Mycoplasma hyopneumoniae. , 1981, American journal of veterinary research.