Roles of SWI1, SWI2, and SWI3 proteins for transcriptional enhancement by steroid receptors.

The SWI1, SWI2, and SWI3 proteins, which are required for regulated transcription of numerous yeast genes, were found also to be essential for rat glucocorticoid receptor function in yeast; the receptor failed to activate transcription in strains with mutations in the SWI1, SWI2, or SWI3 genes. Certain mutations in genes encoding components of chromatin, identified as suppressors of swi mutations, partially relieved the SWI- requirement for receptor function. Immunoprecipitation of glucocorticoid receptor derivatives from wild-type (SWI+) yeast extracts coprecipitated the SWI3 protein; such receptor-SWI3 complexes were not detected in swi1- or swi2- mutant strains, implying that a complex of multiple SWI proteins may associate with the receptor. Prior incubation of a Drosophila embryo transcription extract with the yeast SWI3-specific antibody inhibited receptor function in vitro whereas the antibody had no effect if added after initiation complex formation. Thus, positive regulation by the glucocorticoid receptor in vivo and in vitro appears to require its interaction, at an early step, with one or more SWI proteins.

[1]  M. Carlson,et al.  Yeast SNF2/SWI2, SNF5, and SNF6 proteins function coordinately with the gene-specific transcriptional activators GAL4 and Bicoid. , 1992, Genes & development.

[2]  K. Yamamoto,et al.  Signal transduction and transcriptional regulation by glucocorticoid receptor-LexA fusion proteins. , 1988, Science.

[3]  Thomas C. Kaufman,et al.  brahma: A regulator of Drosophila homeotic genes structurally related to the yeast transcriptional activator SNF2 SWI2 , 1992, Cell.

[4]  K. Yamamoto,et al.  Mammalian glucocorticoid receptor derivatives enhance transcription in yeast. , 1988, Science.

[5]  J. Kennison,et al.  Dosage-dependent modifiers of polycomb and antennapedia mutations in Drosophila. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[6]  E T Young,et al.  The cloning and mapping of ADR6, a gene required for sporulation and for expression of the alcohol dehydrogenase II isozyme from Saccharomyces cerevisiae. , 1987, Genetics.

[7]  G. Bitter,et al.  Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoter. , 1984, Gene.

[8]  S. Lindquist,et al.  Reduced levels of hsp90 compromise steroid receptor action in vivo , 1990, Nature.

[9]  K. Murata,et al.  Transformation of intact yeast cells treated with alkali cations , 1983 .

[10]  K. Yamamoto,et al.  In vitro transcription enhancement by purified derivatives of the glucocorticoid receptor. , 1989, Science.

[11]  G. Hager,et al.  Evidence that nucleosomes on the mouse mammary tumor virus promoter adopt specific translational positions. , 1992, Nucleic acids research.

[12]  I. Herskowitz,et al.  Activation of the yeast HO gene by release from multiple negative controls , 1987, Cell.

[13]  K. Yamamoto,et al.  Signaling and regulation by a mammalian glucocorticoid receptor in Drosophila cells. , 1991, Molecular endocrinology.

[14]  F. Winston,et al.  Changes in histone gene dosage alter transcription in yeast. , 1988, Genes & development.

[15]  E. Young,et al.  The identification and characterization of ADR6, a gene required for sporulation and for expression of the alcohol dehydrogenase II isozyme from Saccharomyces cerevisiae. , 1987, Genetics.

[16]  K. Yamamoto,et al.  An inducible expression vector for both fission and budding yeast. , 1990, Gene.

[17]  W. Soeller,et al.  In vitro transcription of the Drosophila engrailed gene. , 1988, Genes & development.

[18]  I. Herskowitz,et al.  Five SWI genes are required for expression of the HO gene in yeast. , 1984, Journal of molecular biology.

[19]  K. Yamamoto,et al.  Transcription factor interactions: selectors of positive or negative regulation from a single DNA element. , 1990, Science.

[20]  S. Rusconi,et al.  Interference and synergism of glucocorticoid receptor and octamer factors. , 1991, The EMBO journal.

[21]  H. Akil,et al.  Localization of the 90-kDa heat shock protein-binding site within the hormone-binding domain of the glucocorticoid receptor by peptide competition. , 1991, The Journal of biological chemistry.

[22]  K. Yamamoto,et al.  Mapping the HSP90 binding region of the glucocorticoid receptor. , 1990, The Journal of biological chemistry.

[23]  R. Tjian,et al.  Temporal pattern of alcohol dehydrogenase gene transcription reproduced by Drosophila stage-specific embryonic extracts , 1988, Nature.

[24]  J. Thorner,et al.  A presumptive helicase (MOT1 gene product) affects gene expression and is required for viability in the yeast Saccharomyces cerevisiae , 1992, Molecular and cellular biology.

[25]  R. W. Davis,et al.  A steroid-inducible gene expression system for plant cells. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[26]  R. Paro,et al.  The Polycomb protein shares a homologous domain with a heterochromatin-associated protein of Drosophila. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[27]  I. Herskowitz,et al.  A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1 , 1991, Molecular and cellular biology.

[28]  P. Nurse.  Universal control mechanism regulating onset of M-phase , 1990, Nature.

[29]  M. Carlson,et al.  Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[30]  J. T. Kadonaga,et al.  Fractionation of the general RNA polymerase II transcription factors from Drosophila embryos. , 1990, The Journal of biological chemistry.

[31]  R. Nussbaum,et al.  Cloning of human and bovine homologs of SNF2/SWI2: a global activator of transcription in yeast S. cerevisiae. , 1992, Nucleic acids research.

[32]  G. Hager,et al.  Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter , 1991, Molecular and cellular biology.

[33]  K. Yamamoto,et al.  Glucocorticoid receptor mutants that are constitutive activators of transcriptional enhancement , 1987, Nature.

[34]  J. Harmon,et al.  Hormone-free mouse glucocorticoid receptors overexpressed in Chinese hamster ovary cells are localized to the nucleus and are associated with both hsp70 and hsp90. , 1990, The Journal of biological chemistry.

[35]  P. Chambon,et al.  The human oestrogen receptor functions in yeast , 1988, Nature.

[36]  M. Carlson,et al.  An essential Saccharomyces cerevisiae gene homologous to SNF2 encodes a helicase-related protein in a new family , 1992, Molecular and cellular biology.

[37]  B. O’Malley,et al.  The progesterone receptor stimulates cell-free transcription by enhancing the formation of a stable preinitiation complex , 1990, Cell.

[38]  R. Paro,et al.  Imprinting a determined state into the chromatin of Drosophila. , 1990, Trends in genetics : TIG.

[39]  I. Herskowitz,et al.  Identification of a DNA binding factor involved in cell-cycle control of the yeast HO gene , 1989, Cell.

[40]  S. McKnight,et al.  Diversity and specificity in transcriptional regulation: the benefits of heterotypic dimerization. , 1991, Trends in biochemical sciences.

[41]  R. Schüle,et al.  Cooperativity of the glucocorticoid receptor and the CACCC-box binding factor , 1988, Nature.

[42]  K. Yamamoto,et al.  Steroid receptor regulated transcription of specific genes and gene networks. , 1985, Annual review of genetics.

[43]  L. Guarente,et al.  Conservation and evolution of transcriptional mechanisms in eukaryotes. , 1992, Trends in genetics : TIG.

[44]  Tom Maniatis,et al.  GAL4 activates transcription in Drosophila , 1988, Nature.

[45]  P. O’Farrell,et al.  An evolutionarily conserved cyclin homolog from Drosophila rescues yeast deficient in G1 cyclins , 1991, Cell.

[46]  M. Carlson,et al.  Genes affecting the regulation of SUC2 gene expression by glucose repression in Saccharomyces cerevisiae. , 1984, Genetics.

[47]  R. Brent,et al.  A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene , 1984, Nature.

[48]  K. Yamamoto,et al.  Regulatory crosstalk at composite response elements. , 1991, Trends in biochemical sciences.

[49]  Keith R. Yamamoto,et al.  Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element , 1984, Cell.

[50]  R. Paro,et al.  Analysis of the functional role of the Polycomb chromo domain in Drosophila melanogaster. , 1992, Genes & development.

[51]  Gene regulation by steroid hormones , 1989, Cell.

[52]  A. Reik,et al.  Glucocorticoids are required for establishment and maintenance of an alteration in chromatin structure: induction leads to a reversible disruption of nucleosomes over an enhancer. , 1991, The EMBO journal.

[53]  P. Sharp,et al.  Function of a yeast TATA element-binding protein in a mammalian transcription system , 1988, Nature.

[54]  L. Guarente,et al.  Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site , 1983, Cell.

[55]  I. Herskowitz,et al.  Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription , 1992, Cell.

[56]  R. Harrison,et al.  Characterization of a monoclonal antibody to the rat liver glucocorticoid receptor. , 1984, Endocrinology.

[57]  P. Argos,et al.  Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A , 1986, Nature.

[58]  M. Beato,et al.  Nucleosome positioning modulates accessibility of regulatory proteins to the mouse mammary tumor virus promoter , 1990, Cell.

[59]  R. Sikorski,et al.  A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. , 1989, Genetics.

[60]  M. Carlson,et al.  SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae , 1990, Molecular and cellular biology.

[61]  W. Herr,et al.  Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[62]  M. Carlson,et al.  The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes , 1990, Molecular and cellular biology.

[63]  K. Yamamoto,et al.  Mutations in the glucocorticoid receptor zinc finger region that distinguish interdigitated DNA binding and transcriptional enhancement activities. , 1989, Genes & development.

[64]  R. Roeder,et al.  The complexities of eukaryotic transcription initiation: regulation of preinitiation complex assembly. , 1991, Trends in biochemical sciences.