High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain

Abstract High yield production of polyhydroxyalkanoates (PHAs) by Ralstonia eutropha H16 and its recombinant strain PHB−4/pJRDEE32d13 (a PHA-negative mutant harboring Aeromonas caviae PHA synthase gene, phaCAc) from renewable inexpensive soybean oil was investigated. The PHA production by the wild-type strain H16 was achieved with a high dry cells weight (118–126 g/l) and a high poly[(R)-3-hydroxybutyrate] [P(3HB)] content per dry cells of 72–76% (w/w). A copolymer of 3HB with 5 mol% (R)-3-hydroxyhexanoate, P(3HB-co-5 mol% 3HHx), could be produced from soybean oil as a sole carbon source by the recombinant strain PHB−4/pJRDEE32d13 with a high dry cells weight (128–138 g/l) and a high PHA content of 71–74% (w/w). The reproducible results of PHA production in the presence of soybean oil as a sole carbon source was obtained with a high yield at a range of 0.72 to 0.76 g-PHA per g-soybean oil used.

[1]  H. Chang,et al.  Production of poly(3-hydroxybutyrate) by high cell density fed-batch culture of Alcaligenes eutrophus with phospate limitation. , 1997, Biotechnology and bioengineering.

[2]  Takeharu Tsuge.  Metabolic improvements and use of inexpensive carbon sources in microbial production of polyhydroxyalkanoates. , 2002, Journal of bioscience and bioengineering.

[3]  T. Fukui,et al.  Cloning and Analysis of the Poly ( 3-Hydroxybutyrate-co3-Hydroxyhexanoate ) Biosynthesis Genes of Aeromonas caviae , 1996 .

[4]  H. Abe,et al.  Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) , 1995 .

[5]  R J Allen,et al.  The estimation of phosphorus. , 1940, The Biochemical journal.

[6]  Y. Doi,et al.  Production of poly(3-hydroxyalkanoates) by a bacterium of the genus Alcaligenes utilizing long-chain fatty acids , 1992, Applied Microbiology and Biotechnology.

[7]  S H Lee,et al.  Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation. , 2000, Biotechnology and bioengineering.

[8]  T. Fukui,et al.  Biosynthesis of polyhydroxyalkanoates (PHA) by recombinant Ralstonia eutropha and effects of PHA synthase activity on in vivo PHA biosynthesis. , 1999, International journal of biological macromolecules.

[9]  Alexander Steinbüchel,et al.  Perspectives for Biotechnological Production and Utilization of Biopolymers: Metabolic Engineering of Polyhydroxyalkanoate Biosynthesis Pathways as a Successful Example , 2001 .

[10]  T. Fukui,et al.  Efficient production of polyhydroxyalkanoates from plant oils by Alcaligenes eutrophus and its recombinant strain , 1998, Applied Microbiology and Biotechnology.

[11]  Minoru Akiyama,et al.  Environmental life cycle comparison of polyhydroxyalkanoates produced from renewable carbon resources by bacterial fermentation , 2003 .

[12]  T. Iwata,et al.  Mechanical properties of uniaxially cold-drawn films of poly([R]-3-hydroxybutyrate) , 2003 .

[13]  K. Sudesh,et al.  Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters , 2000 .

[14]  H. Schlegel,et al.  The isolation of mutants not accumulating poly-β-hydroxybutyric acid , 2004, Archiv für Mikrobiologie.

[15]  H. Abe,et al.  Biosynthesis and properties of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant strains of Pseudomonas sp. 61-3. , 2000, Biomacromolecules.

[16]  T. Yamane.  Cultivation engineering of microbial bioplastics production , 1992 .

[17]  Sang Yup Lee,et al.  Effect of fermentation performance on the economics of poly(3-hydroxybutyrate) production byAlcaligenes latus , 1998 .

[18]  Chris Somerville,et al.  Production of Polyhydroxyalkanoates, a Family of Biodegradable Plastics and Elastomers, in Bacteria and Plants , 1995, Bio/Technology.