Performance characteristics of a small side‐window photomultiplier in laser single‐photon fluorescence decay measurements

The performance characteristics of the Hamamatsu R928 side‐window photomultiplier tube have been evaluated in the picosecond time domain. This phototube is proposed as an attractive solution to the problem of high time resolution when a sync‐pumped dye laser is combined with the single‐photon time correlation method for determining fluorescence lifetimes and measuring time‐resolved emission spectra and polarization anisotropy. It combines excellent timing characteristics in the single‐photon mode and a small‐to‐negligible wavelength effect with high gain, low noise, and low cost. Twice the resolution previously reported has been obtained.

[1]  H. Lessing,et al.  Rotational-diffusion anomalies in dye solutions from transient-dichroism experiments , 1979 .

[2]  A. Balter,et al.  A method of avoiding wavelength-dependent errors in decay-time measurements , 1979 .

[3]  R. J. Robbins,et al.  Photophysics of Aqueous Tryptophan: pH and Temperature Effects , 1980 .

[4]  A. Visser,et al.  Pulse selection system with electro‐optic modulators applied to mode‐locked cw lasers and time‐resolved single photon counting , 1981 .

[5]  G. Weber.  Polarization of the fluorescence of macromolecules. II. Fluorescent conjugates of ovalbumin and bovine serum albumin. , 1952, The Biochemical journal.

[6]  G. Beck.  Operation of a 1P28 photomultiplier with subnanosecond response time , 1976 .

[7]  A. Olszowski.  Pseudoazulenes: a new large group of compounds with anomalous fluorescence , 1980 .

[8]  J. Penn,et al.  Evaluation of single-photon-counting measurements of excited-state lifetimes. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[9]  S. Provencher,et al.  Double beam fluorescence lifetime spectrometer with subnanosecond resolution: Application to aqueous tryptophan , 1982 .

[10]  A. Jablonski.  Über die Abklingungsvorgänge polarisierter Photolumineszenz , 1961 .

[11]  R. Dowben,et al.  Subnanosecond single photon counting fluorescence spectroscopy using synchronously pumped tunable dye laser excitation. , 1978, The Review of scientific instruments.

[12]  R. Lumry,et al.  High Performance Phase Fluorometer Constructed from Commercial Subunits , 1965 .

[13]  P. Wahl,et al.  Pulsefluorimetry of tyrosyl peptides. , 1978, Biophysical chemistry.

[14]  R. D. Spencer,et al.  Influence of Brownian Rotations and Energy Transfer upon the Measurements of Fluorescence Lifetime , 1970 .

[15]  Colin Lewis,et al.  The Measurement of Short‐Lived Fluorescence Decay Using the Single Photon Counting Method , 1973 .

[16]  Kenneth G. Spears,et al.  Hydrogen bond strengths from solvent-dependent lifetimes of Rose Bengal dye , 1978 .

[17]  R. Bachrach.  A Photon Counting Apparatus for Kinetic and Spectral Measurements , 1972 .

[18]  W. Ware,et al.  Creation and detection of the excited state , 1971 .

[19]  F. Lytle,et al.  Optimization of data-acquistion rates in time-correlated single-photon fluorimetry. , 1979, The Review of scientific instruments.

[20]  Branko Leskovar,et al.  Photon counting system for subnanosecond fluorescence lifetime measurements , 1976 .

[21]  L. Hoffland,et al.  Subnanosecond time-correlated photon counting with tunable lasers. , 1978, The Review of scientific instruments.

[22]  K. Spears,et al.  Rotational diffusion in aprotic and protic solvents , 1978 .

[23]  K. Ghiggino,et al.  Time-resolved fluorescence spectroscopy using pulsed lasers , 1980 .

[24]  S. Kinoshita,et al.  High‐performance, time‐correlated single photon counting apparatus using a side‐on type photomultiplier , 1982 .

[25]  J. M. Morris,et al.  Picosecond Fluorescence Studies of Xanthene Dyes , 1977 .

[26]  P. Wahl,et al.  Fluorescence depolarization of rabbit gamma globulin conjugates. , 1967, Journal of molecular biology.

[27]  P. Wahl,et al.  The wavelength dependence of the response of a pulse fluorometer using the single photoelectron counting method , 1974 .

[28]  S. Kinoshita,et al.  Subnanosecond fluorescence‐lifetime measuring system using single photon counting method with mode‐locked laser excitation , 1981 .