Development of High Meat Quality Using Microsatellite Markers in Berkshire Pigs

본 연구는 버크셔종의 육질에 대한 특성을 분석하여 국내 돈육시장에서의 적합성을 확인하고, MS marker를 이용한 육질 우수 개체 선발의 효율성에 대해서 분석하였다. 버크셔 323두를 동일 사양조건에서 사육하고 도축하여 육질형질을 분석하고, 혈액으로부터 genomic DNA를 분리하여 50개의 MS marker에 대한 유전자형을 분석하였다. 버크셔종은 국내에서 가장 많이 이용되고 있는 듀록, 요크셔, 랜드레이스 종보다도 육질에 있어서 더욱 우수한 특성을 나타내었다. 특히 도축 24시간 후 pH 값은 평균 $5.88{\pm}0.01$ 로 대단히 높게 나타났고, 지방함량의 경우에도 $2.878{\pm}0.06$ 로 확인되었다. MS marker와의 연관성을 확인한 결과, 14개 MS marker가 육질형질과 연관성을 있는 것으로 나타났다(p 【In this study, the efficiency of microsatellite (MS) markers for pork quality was examined and further, their suitability to domestic pork industry also was verified, by measuring meat quality parameters of Berkshire breeds. A total of 323 pigs of Berkshire breeds were slaughtered and subjected to meat quality evaluation. In addition, the genomic DNAs from blood samples of slaughtered pigs were used for genotyping analysis of 50 MS markers. The results revealed that Berkshire breeds have excellent meat quality, compared with the popular domestic breeds such as Duroc, Yorkshire, and Landrace. Noticeably, the Berkshire breeds exhibited a significant post-mortem pH24hr ( $5.88{\pm}0.01$ ) and fat content ( $2.878{\pm}0.06$ ). Through the linkage analysis between MS markers, 14 MS markers showed significant association with meat quality traits (p】

[1]  M. Rothschild,et al.  The genetics of the pig , 2011 .

[2]  Shirley Anderson,et al.  Determination of fat, moisture, and protein in meat and meat products by using the FOSS FoodScan Near-Infrared Spectrophotometer with FOSS Artificial Neural Network Calibration Model and Associated Database: collaborative study. , 2007, Journal of AOAC International.

[3]  K Schellander,et al.  A genome scan reveals QTL for growth, fatness, leanness and meat quality in a Duroc-Pietrain resource population. , 2007, Animal genetics.

[4]  M. Georges,et al.  Results of a whole-genome quantitative trait locus scan for growth, carcass composition and meat quality in a porcine four-way cross. , 2006, Animal genetics.

[5]  M. Georges,et al.  The callipyge mutation enhances bidirectional long-range DLK1-GTL2 intergenic transcription in cis. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[6]  M. Groenen,et al.  Identification of quantitative trait loci for carcass composition and pork quality traits in a commercial finishing cross. , 2006, Journal of animal science.

[7]  G. Rohrer,et al.  A genome scan for loci affecting pork quality in a Duroc-Landrace F population. , 2006, Animal genetics.

[8]  M. Rothschild Porcine genomics delivers new tools and results: this little piggy did more than just go to market. , 2004, Genetical research.

[9]  Leif Andersson,et al.  A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig , 2003, Nature.

[10]  Hou Jian Relationships Between Microsatellite DNA Markers and Pork Quality Traits , 2003 .

[11]  R. Fries,et al.  Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M F Rothschild,et al.  Correlations among selected pork quality traits. , 2002, Journal of animal science.

[13]  Michel Georges,et al.  Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. , 2002, Nature Reviews Genetics.

[14]  M. Groenen,et al.  Detection and characterization of quantitative trait loci for meat quality traits in pigs. , 2001, Journal of animal science.

[15]  J. Dekkers,et al.  A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition , 2001, Mammalian Genome.

[16]  H. J. Andersen,et al.  Relationship between RN(-) genotype and drip loss in meat from Danish pigs. , 2000, Meat science.

[17]  C. Rogel-Gaillard,et al.  A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle. , 2000, Science.

[18]  L. Andersson,et al.  A high-density linkage map of the RN region in pigs , 2000, Genetics Selection Evolution.

[19]  M. Rothschild,et al.  Genetics of meat and carcass traits. , 1998 .

[20]  G. Offer,et al.  Modelling of the formation of pale, soft and exudative meat: Effects of chilling regime and rate and extent of glycolysis. , 1991, Meat science.

[21]  J. Elsen,et al.  Evidence for a new major gene influencing meat quality in pigs. , 1990, Genetical research.

[22]  E. Laakkonen,et al.  LOW-TEMPERATURE, LONG-TIME HEATING OF BOVINE MUSCLE 1. Changes in Tenderness, Water-Binding Capacity, pH and Amount of Water-Soluble Components , 1970 .