A human colon carcinoma cell line exhibits adhesive interactions with P-selectin under fluid flow via a PSGL-1-independent mechanism.

It has been postulated that endothelial cell adhesion molecules involved in leukocyte recruitment play a role in metastasis. Using an in vitro flow model, we studied the adhesion of the human colon carcinoma cell line KM12-L4 to P-selectin, an inducible endothelial-expressed adhesion molecule involved in leukocyte recruitment. Recombinant forms of P-selectin and Chinese hamster ovary cells stably expressing P-selectin supported attachment and rolling of KM12-L4 cells at 1 to 2 dynes/cm2. The adhesive interactions to P-selectin were abolished by pretreatment of the KM12-L4 cells with neuraminidase but were unaltered by pretreatment of the KM12-L4 cells with O-sialoglycoprotein endopeptidase, an enzyme that cleaves mucin type glycoproteins such as P-selectin glycoprotein ligand-1 (PSGL-1). PSGL-1 is the only counter-receptor for P-selectin known to mediate myeloid cell adhesion to P-selectin under flow. Flow cytometric and Northern blot analyses revealed that KM12-L4 cells did not express PSGL-1 and monoclonal antibody PL1, a function-blocking monoclonal antibody to PSGL-1, had no inhibitory effect on KM12-L4 adhesion to P-selectin under flow. Compared with HL-60 cells, which express PSGL-1, the KM12-L4 cells exhibited a slightly lower rate of attachment to P-selectin and rolled at a significantly higher velocity. In summary, KM12-L4 human colon carcinoma cells interact with P-selectin, under flow, through a PSGL-1-independent adhesion pathway.

[1]  H. Kleinman,et al.  E‐selectin‐mediated dynamic interactions of breast‐and colon‐cancer cells with endothelial‐cell monolayers , 2006, International journal of cancer.

[2]  K. Comess,et al.  A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding , 1995, Cell.

[3]  Brian Seed,et al.  PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus , 1995, Cell.

[4]  A. Varki,et al.  Differential colon cancer cell adhesion to E-, P-, and L-selectin: role of mucin-type glycoproteins. , 1995, Cancer research.

[5]  M. U. Nollert,et al.  Neutrophils use both shared and distinct mechanisms to adhere to selectins under static and flow conditions. , 1995, The Journal of clinical investigation.

[6]  E. Butcher,et al.  A central role for microvillous receptor presentation in leukocyte adhesion under flow , 1995, Cell.

[7]  G. Vachino,et al.  P-selectin Glycoprotein Ligand-1 Is the Major Counter-receptor for P-selectin on Stimulated T Cells and Is Widely Distributed in Non-functional Form on Many Lymphocytic Cells * , 1995, The Journal of Biological Chemistry.

[8]  P. Pizcueta,et al.  L-selectin-deficient mice have impaired leukocyte recruitment into inflammatory sites , 1995, The Journal of experimental medicine.

[9]  T. Maniatis,et al.  The proteasome pathway is required for cytokine-induced endothelial-leukocyte adhesion molecule expression. , 1995, Immunity.

[10]  R. Cummings,et al.  P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin , 1995, The Journal of cell biology.

[11]  T. Springer,et al.  Distinct cell surface ligands mediate T lymphocyte attachment and rolling on P and E selectin under physiological flow , 1994, The Journal of cell biology.

[12]  T. Carlos,et al.  Leukocyte-endothelial adhesion molecules. , 1994, Blood.

[13]  R. Cummings,et al.  The P-selectin glycoprotein ligand from human neutrophils displays sialylated, fucosylated, O-linked poly-N-acetyllactosamine. , 1994, The Journal of biological chemistry.

[14]  F. Luscinskas,et al.  Integrins as dynamic regulators of vascular function , 1994, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[15]  A. Varki,et al.  Selectin ligands. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[16]  P. Altevogt,et al.  Heat-stable antigen (CD24) as ligand for mouse P-selectin. , 1994, International immunology.

[17]  G S Kansas,et al.  Monocyte rolling, arrest and spreading on IL-4-activated vascular endothelium under flow is mediated via sequential action of L-selectin, beta 1-integrins, and beta 2-integrins , 1994, The Journal of cell biology.

[18]  D. A. Hammer,et al.  Receptor-mediated binding of IgE-sensitized rat basophilic leukemia cells to antigen-coated substrates under hydrodynamic flow. , 1994, Biophysical journal.

[19]  T. Springer.  Traffic signals for lymphocyte recirculation and leukocyte emigration: The multistep paradigm , 1994, Cell.

[20]  B. Furie,et al.  Expression cloning of a functional glycoprotein ligand for P-selectin , 1993, Cell.

[21]  T. Springer,et al.  Neutrophils roll on E-selectin. , 1993, Journal of immunology.

[22]  L. McIntire,et al.  E-selectin supports neutrophil rolling in vitro under conditions of flow. , 1993, The Journal of clinical investigation.

[23]  A. Remuzzi,et al.  Rolling and adhesion of human tumor cells on vascular endothelium under physiological flow conditions. , 1993, The Journal of clinical investigation.

[24]  S. Hemmerich,et al.  Binding of L-selectin to the vascular sialomucin CD34. , 1993, Science.

[25]  T. Mayadas,et al.  Leukocyte rolling and extravasation are severely compromised in P selectin-deficient mice , 1993, Cell.

[26]  J. P. Stone,et al.  P-selectin mediates adhesion of platelets to neuroblastoma and small cell lung cancer. , 1993, The Journal of clinical investigation.

[27]  M. Fukuda,et al.  E-selectin-dependent adhesion efficiency of colonic carcinoma cells is increased by genetic manipulation of their cell surface lysosomal membrane glycoprotein-1 expression levels. , 1993, The Journal of biological chemistry.

[28]  R. Cummings,et al.  Characterization of a specific ligand for P-selectin on myeloid cells. A minor glycoprotein with sialylated O-linked oligosaccharides. , 1993, The Journal of biological chemistry.

[29]  R. Cummings,et al.  Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells , 1992, The Journal of cell biology.

[30]  D. Hammer,et al.  Simulation of cell rolling and adhesion on surfaces in shear flow: general results and analysis of selectin-mediated neutrophil adhesion. , 1992 .

[31]  A. Aruffo,et al.  Granule membrane protein 140 (GMP140) binds to carcinomas and carcinoma-derived cell lines. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[32]  D. Sutherland,et al.  Cleavage of the cell-surface O-sialoglycoproteins CD34, CD43, CD44, and CD45 by a novel glycoprotease from Pasteurella haemolytica. , 1992, Journal of immunology.

[33]  P. Mattila,et al.  Recombinant E-selectin-protein mediates tumor cell adhesion via sialyl-Le(a) and sialyl-Le(x). , 1992, Biochemical and biophysical research communications.

[34]  K. Handa,et al.  Selectin GMP-140 (CD62; PADGEM) binds to sialosyl-Le(a) and sialosyl-Le(x), and sulfated glycans modulate this binding. , 1991, Biochemical and biophysical research communications.

[35]  E. Butcher.  Leukocyte-endothelial cell recognition: Three (or more) steps to specificity and diversity , 1991, Cell.

[36]  F. Luscinskas,et al.  Leukocyte adhesion molecule-1 (LAM-1, L-selectin) interacts with an inducible endothelial cell ligand to support leukocyte adhesion. , 1991, Journal of immunology.

[37]  B. Seed,et al.  CD62/P-selectin recognition of myeloid and tumor cell sulfatides , 1991, Cell.

[38]  R. Cummings,et al.  The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells , 1991, The Journal of cell biology.

[39]  Louis J. Picker,et al.  The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140 , 1991, Cell.

[40]  J D Chambers,et al.  Two-step model of leukocyte-endothelial cell interaction in inflammation: distinct roles for LECAM-1 and the leukocyte beta 2 integrins in vivo. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[41]  C. Mackay,et al.  Function and evolutionary conservation of distinct epitopes on the leukocyte adhesion molecule-1 (TQ-1, Leu-8) that regulate leukocyte migration. , 1991, Journal of immunology.

[42]  S. Hakomori,et al.  CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[43]  T. Springer,et al.  Leukocytes roll on a selectin at physiologic flow rates: Distinction from and prerequisite for adhesion through integrins , 1991, Cell.

[44]  A. Varki,et al.  GMP-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction , 1991, The Journal of cell biology.

[45]  B. Seed,et al.  Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells. , 1990, Science.

[46]  K. Shitara,et al.  Detailed characterization of reactivities of anti-gastric cancer monoclonal antibodies to carbohydrate antigen. , 1990, Anticancer research.

[47]  I. Fidler,et al.  In vivo selection of highly metastatic cells from surgical specimens of different primary human colon carcinomas implanted into nude mice. , 1988, Cancer research.

[48]  R. Cotran,et al.  Identification of an inducible endothelial-leukocyte adhesion molecule. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[49]  R. G. Cox,et al.  Slow viscous motion of a sphere parallel to a plane wall , 1967 .

[50]  D. Hammer,et al.  Lifetime of the P-selectin-carbohydrate bond and its response to tensile force in hydrodynamic flow , 1995, Nature.

[51]  M. Polly.  CD62 and endothelial cell-liukocyte adhesion molicule 1 (ELAM-1) recognize the same carbohy drate ligand, sialyl-Lewis , 1991 .

[52]  C W Smith,et al.  Effect of venous shear stress on CD18-mediated neutrophil adhesion to cultured endothelium. , 1990, Blood.