Droplet digital PCR assay provides intrahepatic HBV cccDNA quantification tool for clinical application

[1]  F. Zoulim,et al.  Quantification and epigenetic evaluation of the residual pool of hepatitis B covalently closed circular DNA in long-term nucleoside analogue-treated patients , 2020, Scientific Reports.

[2]  T. Berg,et al.  Composition of HBsAg is predictive of HBsAg loss during treatment in patients with HBeAg-positive chronic hepatitis B. , 2020, Journal of hepatology.

[3]  E. Hatano,et al.  Proposed model for the prediction of intrahepatic covalently closed circular DNA level in patients with chronic hepatitis B , 2018, Hepatology research : the official journal of the Japan Society of Hepatology.

[4]  A. Amoroso,et al.  Quantitation of HBV cccDNA in anti-HBc-positive liver donors by droplet digital PCR: A new tool to detect occult infection. , 2018, Journal of hepatology.

[5]  Song-Mei Liu,et al.  A Highly Sensitive and Robust Method for Hepatitis B Virus Covalently Closed Circular DNA Detection in Single Cells and Serum. , 2018, The Journal of molecular diagnostics : JMD.

[6]  C. Fletcher,et al.  Normalization of cell associated antiretroviral drug concentrations with a novel RPP30 droplet digital PCR assay , 2018, Scientific Reports.

[7]  Zhan-qing Zhang,et al.  Serum Hepatitis B Virus DNA, RNA, and HBsAg: Which Correlated Better with Intrahepatic Covalently Closed Circular DNA before and after Nucleos(t)ide Analogue Treatment? , 2017, Journal of Clinical Microbiology.

[8]  M. Pitman,et al.  Pancreatic cancer: Circulating Tumor Cells and Primary Tumors show Heterogeneous KRAS Mutations , 2017, Scientific Reports.

[9]  M. Yuen,et al.  Hepatitis B virus core‐related antigen as a surrogate marker for covalently closed circular DNA , 2017, Liver international : official journal of the International Association for the Study of the Liver.

[10]  A. Lohse,et al.  Proliferation of primary human hepatocytes and prevention of hepatitis B virus reinfection efficiently deplete nuclear cccDNA in vivo , 2017, Gut.

[11]  Libo Yan,et al.  Serum hepatitis B core-related antigen is a satisfactory surrogate marker of intrahepatic covalently closed circular DNA in chronic hepatitis B , 2017, Scientific Reports.

[12]  Hong Tang,et al.  Serum hepatitis B core-related antigen is a satisfactory surrogate marker of intrahepatic covalently closed circular DNA in chronic hepatitis B , 2017, Scientific Reports.

[13]  M. Yuen,et al.  Reduction of covalently closed circular DNA with long-term nucleos(t)ide analogue treatment in chronic hepatitis B. , 2017, Journal of hepatology.

[14]  D. Dieterich,et al.  A Treatment Algorithm for the Management of Chronic Hepatitis B Virus Infection in the United States: 2015 Update. , 2015, Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association.

[15]  Y. Karino,et al.  Characterization of novel entecavir resistance mutations. , 2015, Journal of hepatology.

[16]  Liang Yan,et al.  A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system , 2015, Biotechnology Letters.

[17]  T. Liang,et al.  Specific and Nonhepatotoxic Degradation of Nuclear Hepatitis B Virus cccDNA , 2014, Science.

[18]  S. Litwin,et al.  Duck hepatitis B virus covalently closed circular DNA appears to survive hepatocyte mitosis in the growing liver. , 2013, Virology.

[19]  Christopher M. Hindson,et al.  Absolute quantification by droplet digital PCR versus analog real-time PCR , 2013, Nature Methods.

[20]  Jeff Mellen,et al.  High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number , 2011, Analytical chemistry.

[21]  N. Terrault.  Benefits and risks of combination therapy for hepatitis B , 2009, Hepatology.

[22]  R. Chung,et al.  Monitoring during and after antiviral therapy for hepatitis B , 2009, Hepatology.

[23]  M. Sugiyama,et al.  Direct cytopathic effects of particular hepatitis B virus genotypes in severe combined immunodeficiency transgenic with urokinase-type plasminogen activator mouse with human hepatocytes. , 2009, Gastroenterology.

[24]  N. Terrault.  Benefits and Risks of Combination Therapy for Hepatitis , 2009 .

[25]  V. Wong,et al.  Serum hepatitis B surface antigen quantitation can reflect hepatitis B virus in the liver and predict treatment response. , 2007, Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association.

[26]  M. Yuen,et al.  Hepatitis B Virus Core-Related Antigens as Markers for Monitoring Chronic Hepatitis B Infection , 2007, Journal of Clinical Microbiology.

[27]  A. Cuconati,et al.  Hepatitis B virus e antigen production is dependent upon covalently closed circular (ccc) DNA in HepAD38 cell cultures and may serve as a cccDNA surrogate in antiviral screening assays. , 2006, Antiviral research.

[28]  N. Izumi,et al.  Influence of hepatitis B virus genotypes on the intra‐ and extracellular expression of viral DNA and antigens , 2006, Hepatology.

[29]  S. Yagi,et al.  Hepatitis B Virus DNA-negative Dane Particles Lack Core Protein but Contain a 22-kDa Precore Protein without C-terminal Arginine-rich Domain* , 2005, Journal of Biological Chemistry.

[30]  M. Yuen,et al.  Quantitation of covalently closed circular hepatitis B virus DNA in chronic hepatitis B patients , 2004, Hepatology.

[31]  T. Asahara,et al.  Near completely humanized liver in mice shows human-type metabolic responses to drugs. , 2004, The American journal of pathology.

[32]  R. Gish,et al.  A case‐control study for differences among hepatitis B virus infections of genotypes A (subtypes Aa and Ae) and D , 2004, Hepatology.

[33]  W. Piao,et al.  Dynamic analysis of hepatitis B virus DNA and its antigens in 2.2.15 cells , 2004, Journal of viral hepatitis.

[34]  S. Litwin,et al.  Kinetics of Hepadnavirus Loss from the Liver during Inhibition of Viral DNA Synthesis , 2001, Journal of Virology.

[35]  R. Kawaguchi,et al.  Quantitation of Hepatitis B Virus Genomic DNA by Real-Time Detection PCR , 1999, Journal of Clinical Microbiology.

[36]  M. Mayer,et al.  A new set of useful cloning and expression vectors derived from pBlueScript. , 1995, Gene.