Occurrence of Chlamydia spp. in Conjunctival Samples of Stray Cats in Timișoara Municipality, Western Romania

Despite the widespread public health concern about stray cats serving as reservoirs for zoonotic agents, little is known about the effect of urban and peri-urban landscapes on exposure risk. We conducted this study to monitor the presence of Chlamydia spp. in stray cats, with or without conjunctivitis, living in Timișoara Municipality, Western Romania, using staining and PCR methods. A total of 95 cats were enrolled, and conjunctival samples were harvested from 68 clinically healthy cats and another 27 cats presenting with clinical signs of conjunctivitis. Overall, we found that 65.3% (62/95) of the cats tested positive for Chlamydia spp. by PCR. Chlamydia spp. were detected in 45/95 conjunctival samples using a standard Giemsa stain, compared with 62/95 using PCR (Cohen’s kappa index = 0.308; p = 0.0640). Of the cats that tested positive by PCR, 72.6% (45/62) were asymptomatic, and another 27.4% (17/62) expressed clinical signs of conjunctivitis. We found no significant difference between (p > 0.05) the distribution of infection and the recorded epidemiological data (sex, breed, age, territorial distribution, or sampling season). However, the Chlamydia spp. detection frequency was significantly higher in asymptomatic than in symptomatic cats (p = 0.0383). The obtained results increase the level of concern and awareness about the possible zoonotic potential of this pathogen and highlight that urban stray cats can be essential sources of feline chlamydiosis.

[1]  J. Carvalho,et al.  Urban landscape and infection risk in free‐roaming cats , 2022, Zoonoses and public health.

[2]  A. Nururrozi,et al.  Investigation of chlamydophilosis from naturally infected cats , 2021, Journal of veterinary science.

[3]  T. Pesch,et al.  Occurrence of Chlamydiaceae and Chlamydia felis pmp9 Typing in Conjunctival and Rectal Samples of Swiss Stray and Pet Cats , 2021, Pathogens.

[4]  M. E. El Zowalaty,et al.  Zoonotic Diseases: Etiology, Impact, and Control , 2020, Microorganisms.

[5]  Orly Razgour,et al.  Emerging zoonotic diseases originating in mammals: a systematic review of effects of anthropogenic land‐use change , 2020, Mammal review.

[6]  J. Plutzer,et al.  Molecular detection of hemotropic mycoplasmas (hemoplasmas) in domestic cats (Felis catus) in Romania , 2020, BMC Veterinary Research.

[7]  K. Sachse,et al.  Recent Advances in Epidemiology, Pathology and Immunology of Veterinary Chlamydiae , 2020 .

[8]  Gilbert GREUB,et al.  International Committee on Systematics of Prokaryotes (ICSP) Subcommittee on the taxonomy of Chlamydiae. Minutes of the closed meeting, 5 July 2018, Woudschoten, Zeist, The Netherlands. , 2019, International journal of systematic and evolutionary microbiology.

[9]  M. Halán,et al.  Impact of way of life and environment on the prevalence of Chlamydia felis in cats as potentional sources of infection for humans. , 2019, Annals of agricultural and environmental medicine : AAEM.

[10]  S. Esmaeilzadeh,et al.  Molecular Detection of Chlamydia felis in Cats in Ahvaz, Iran. , 2019, Archives of Razi Institute.

[11]  W. Wong,et al.  Chlamydiaceae: Diseases in Primary Hosts and Zoonosis , 2019, Microorganisms.

[12]  P. Fleming,et al.  A Case of Letting the Cat out of The Bag—Why Trap-Neuter-Return Is Not an Ethical Solution for Stray Cat (Felis catus) Management , 2019, Animals : an open access journal from MDPI.

[13]  N. Gottdenker,et al.  Disentangling the link between supplemental feeding, population density, and the prevalence of pathogens in urban stray cats , 2018, PeerJ.

[14]  K. Gruszynski,et al.  Zoonotic disease transmission associated with feral cats in a metropolitan area: A geospatial analysis , 2018, Zoonoses and public health.

[15]  L. Athanasiou,et al.  Conjunctival cytology assessment in dogs and cats. Sampling, diagnostic techniques and findings , 2018 .

[16]  R. Gálvez,et al.  Implications of zoonotic and vector-borne parasites to free-roaming cats in central Spain. , 2018, Veterinary parasitology.

[17]  A. Pospischil,et al.  A Review on Chlamydial Diseases in Animals: Still a Challenge for Pathologists? , 2018, Veterinary pathology.

[18]  C. Bogdan,et al.  Follicular Conjunctivitis due to Chlamydia felis—Case Report, Review of the Literature and Improved Molecular Diagnostics , 2017, Front. Med..

[19]  CongWei,et al.  First Report of Toxoplasma gondii, Dirofilaria immitis, and Chlamydia felis Infection in Stray and Companion Cats in Northeastern and Eastern China , 2016 .

[20]  P. Melville,et al.  Chlamydia felis: Lack of association between clinical signs and the presence of the cryptic plasmid. , 2016, Microbial pathogenesis.

[21]  M. André,et al.  Detecção de Chlamydophila felis e Herpesvirus felino tipo 1 em felídeo não doméstico no Brasil , 2016 .

[22]  L. Sandmeyer,et al.  Diagnostic Ophthalmology. , 2016, The Canadian veterinary journal = La revue veterinaire canadienne.

[23]  Yuanhuan Kang,et al.  First Report of Toxoplasma gondii, Dirofilaria immitis, and Chlamydia felis Infection in Stray and Companion Cats in Northeastern and Eastern China. , 2016, Vector Borne and Zoonotic Diseases.

[24]  A. Sing.  Cat-Related Zoonoses: Killing You Softly with Feces and Fleas , 2015 .

[25]  S. McGorray,et al.  Prevalence of upper respiratory pathogens in four management models for unowned cats in the Southeast United States. , 2014, Veterinary journal.

[26]  R. V. Van Gelder,et al.  Role of Molecular Diagnostics in Ocular Microbiology , 2013, Current Ophthalmology Reports.

[27]  Xing-Quan Zhu,et al.  Chlamydia felis exposure in companion dogs and cats in Lanzhou, China: a public health concern , 2013, BMC Veterinary Research.

[28]  R. Hofmann-Lehmann,et al.  GAPDH Pseudogenes and the Quantification of Feline Genomic DNA Equivalents , 2013, Molecular biology international.

[29]  Dylan S. Small,et al.  The Effects of City Streets on an Urban Disease Vector , 2013, PLoS Comput. Biol..

[30]  C. Schrader,et al.  PCR inhibitors – occurrence, properties and removal , 2012, Journal of applied microbiology.

[31]  B. S. Holst,et al.  Evaluation of cytologic findings in feline conjunctivitis. , 2012, Veterinary clinical pathology.

[32]  M. Halán,et al.  Chlamydophila felis in Cats – Are the Stray Cats Dangerous Source of Infection? , 2011, Zoonoses and public health.

[33]  S. Little.  A review of feline leukemia virus and feline immunodeficiency virus seroprevalence in cats in Canada. , 2011, Veterinary immunology and immunopathology.

[34]  A. Pospischil,et al.  Chlamydia in canine or feline coronary arteriosclerotic lesions , 2011, BMC Research Notes.

[35]  P. Apfalter,et al.  Detection of Chlamydophila pneumoniae in cats with conjunctivitis. , 2011, Veterinary ophthalmology.

[36]  A. Rodolakis,et al.  Zoonotic potential of Chlamydophila. , 2010, Veterinary microbiology.

[37]  G. Rohde,et al.  Chlamydial zoonoses. , 2010, Deutsches Arzteblatt international.

[38]  E. Thiry,et al.  Chlamydophila Felis Infection: ABCD Guidelines on Prevention and Management , 2009, Journal of feline medicine and surgery.

[39]  Kate E. Jones,et al.  Global trends in emerging infectious diseases , 2008, Nature.

[40]  K. Gaston,et al.  Avian assemblage structure and domestic cat densities in urban environments , 2007 .

[41]  M. Lappin,et al.  Prevalence of feline herpesvirus 1, Chlamydophila felis, and Mycoplasma spp DNA in conjunctival cells collected from cats with and without conjunctivitis. , 2007, American journal of veterinary research.

[42]  M. Hattori,et al.  Genome sequence of the cat pathogen, Chlamydophila felis. , 2006, DNA research : an international journal for rapid publication of reports on genes and genomes.

[43]  L. Polley.  Navigating parasite webs and parasite flow: emerging and re-emerging parasitic zoonoses of wildlife origin. , 2005, International journal for parasitology.

[44]  J. Sykes.  Feline chlamydiosis. , 2005, Clinical techniques in small animal practice.

[45]  G. Browning,et al.  Is Chlamydophila felis a significant zoonotic pathogen? , 2004, Australian veterinary journal.

[46]  A. Di Francesco,et al.  Prevalence of Chlamydophila felis by PCR among healthy pet cats in Italy. , 2004, The new microbiologica.

[47]  E. Zini,et al.  Prevalence of Chlamydophila felis and feline herpesvirus 1 in cats with conjunctivitis in northern Italy. , 2003, Journal of veterinary internal medicine.

[48]  A. Pospischil,et al.  Detection of novel chlamydiae in cats with ocular disease. , 2003, American journal of veterinary research.

[49]  H. Kitagawa,et al.  Seroepidemiological Investigation of Feline Chlamydiosis in Cats and Humans in Japan , 2000, Microbiology and immunology.

[50]  B. Willett,et al.  A comparison of DNA amplification, isolation and serology for the detection of Chlamydia psittaci infection in cats , 1998, Veterinary Record.

[51]  G. Patronek.  Free-roaming and feral cats--their impact on wildlife and human beings. , 1998, Journal of the American Veterinary Medical Association.

[52]  G. E. Jones,et al.  Pathogenesis of Chlamydia psittaci infection in sheep: detection of the organism in a serial study of the lymph node. , 1996, Journal of comparative pathology.