Computer vision and graphics in fluorescence microscopy

The focus of this paper is on the visualization of intracellular structures in 3-D, dual labelled, fluorescent images, and the quantification of the spatial relationships among these structures. Specifically, a local, fast deformable model has been developed which finds the membrane of a cell. Two visualization algorithms have also been developed. One is a surface-based algorithm which converts voxel data into planar surfaces and hence permits the visualization of the cell membrane (as found by the deformable model) in conjunction with the original data. The second is a 3-D voxel-based algorithm which permits the simultaneous visualization of two data sets and is optimized to emphasize the extent to which the data in two 3-D images (one from each fluorescent label) co-localize. In addition, a quantitative analysis of overlap between two data volumes has also been performed. The application of the developed tools to several biologically motivated problems is discussed.

[1]  D. Burr.  A dynamic model for image registration , 1981 .

[2]  Keith Waters,et al.  A muscle model for animation three-dimensional facial expression , 1987, SIGGRAPH.

[3]  Lawrence M. Lifshitz,et al.  Coupling of the Na+/Ca2+exchanger, Na+/K+ pump and sarcoplasmic reticulum in smooth muscle , 1993, Nature.

[4]  Demetri Terzopoulos,et al.  Regularization of Inverse Visual Problems Involving Discontinuities , 1986, IEEE Transactions on Pattern Analysis and Machine Intelligence.

[5]  Guy Marchal,et al.  Delineation of neuroanatomical objects using deformable models , 1989 .

[6]  F S Fay,et al.  Calcium gradients underlying polarization and chemotaxis of eosinophils. , 1991, Science.

[7]  F. S. Fay,et al.  3D Fluorescence imaging of single cells using image restoration , 1990 .

[8]  Jerry Weil,et al.  The synthesis of cloth objects , 1986, SIGGRAPH.

[9]  John Platt,et al.  Heating and melting deformable models (from goop to glop) , 1989 .

[10]  F S Fay,et al.  Role of Na(+)-Ca2+ exchanger in beta-adrenergic relaxation of single smooth muscle cells. , 1991, Annals of the New York Academy of Sciences.

[11]  John C. Platt,et al.  Constraints methods for flexible models , 1988, SIGGRAPH.

[12]  Gauch Jm.  Coherent image shape description via the intensity axis of symmetry. , 1991 .

[13]  M. Levoy.  Volume Rendering Display of Surfaces from Volume Data , 1988 .

[14]  Laurent D. Cohen,et al.  Building Highly Structured Volume Representations in 3D Medical Images , 1989 .

[15]  Demetri Terzopoulos,et al.  Modeling inelastic deformation: viscolelasticity, plasticity, fracture , 1988, SIGGRAPH.

[16]  S. Y. Chen,et al.  Improvement on dynamic elastic interpolation technique for reconstructing 3-D objects from serial cross sections [biomedical application]. , 1990, IEEE transactions on medical imaging.

[17]  John M. Gauch,et al.  Comparison of implementation strategies for deformable surfaces in computer vision , 1992, Optics & Photonics.

[18]  Carl Richard Feynman,et al.  Modeling the appearance of cloth , 1986 .

[19]  Alan H. Barr,et al.  Global and local deformations of solid primitives , 1984, SIGGRAPH.