Coleopterofauna found on fresh and frozen rabbit carcasses in Curitiba, Paraná, Brazil.

Many arthropod species are associated with carrion and some of them can be used as forensic indicators in murder investigations to estimate the time of death. Different physical conditions of cadavers may influence the fauna and the importance of freezing and thawing is unknown. The present paper sought to survey the Coleoptera species encountered in frozen and fresh rabbit carcasses, at a forest in Curitiba, Brazil. Four rabbit carcasses, two of them fresh and the other frozen were used. The coleopterofauna was sampled daily, and analyzed using non-parametric tests. A total of 666 beetles were sampled, belonging to 28 species in 10 families. Most of the beetles captured were larvae of Oxelytrum spp. (433) which are known to be necrophagous. The two frozen carcasses accounted for most of the beetles (338 and 180) in comparison with the two fresh carcasses (103 and 45). The tests were based on the most abundant species. Oxelytrum spp. median differed significantly between carcasses (H = 12.47844; p = 0.0059). The two fresh carcasses differed significantly (U = 190.0; p = 0.00019), but there was no significant difference between the frozen carcasses (U = 336.0; p = 0.29755). The data indicate that the freezing process prevents certain species to colonize carcasses, in this case with the dominance of species of Oxelytrum. These data also indicate that careful attention is necessary before using frozen carcasses in forensic entomology studies.

[1]  K. M. Mise,et al.  Diagnosis and key of the main families and species of South American Coleoptera of forensic importance , 2009 .

[2]  S. Forbes,et al.  Freezing skeletal muscle tissue does not affect its decomposition in soil: evidence from temporal changes in tissue mass, microbial activity and soil chemistry based on excised samples. , 2009, Forensic science international.

[3]  F. B. Matos,et al.  A vegetação na Reserva Mata Viva, Curitiba, Paraná, Brasil , 2008 .

[4]  R. F. Krüger,et al.  Insects of forensic importance from Rio Grande do Sul state in southern Brazil , 2008 .

[5]  K. M. Mise,et al.  Longer decomposition process and the influence on Coleoptera fauna associated with carcasses. , 2008, Brazilian journal of biology = Revista brasleira de biologia.

[6]  M. O. Moura,et al.  Levantamento da fauna de Coleoptera que habita a carcaça de Sus scrofa L., em Curitiba, Paraná , 2007 .

[7]  A. Gunn.  Essential Forensic Biology , 2006 .

[8]  J. Wallman,et al.  A comparison of frozen/thawed and fresh food substrates in development of Calliphora augur (Diptera: Calliphoridae) larvae , 2006, International Journal of Legal Medicine.

[9]  M. Elgar,et al.  Yearly activity patterns in southern Victoria (Australia) of seasonally active carrion insects. , 2003, Forensic science international.

[10]  M. Barreto,et al.  Flies (Calliphoridae, Muscidae) and beetles (Silphidae) from human cadavers in Cali, Colombia. , 2002, Memorias do Instituto Oswaldo Cruz.

[11]  M. I. Marchenko.  Medicolegal relevance of cadaver entomofauna for the determination of the time of death. , 2001, Forensic science international.

[12]  P. Kulshrestha,et al.  Use of beetles in forensic entomology. , 2001, Forensic science international.

[13]  S. Mazur.  Review of the Histeridae (Coleoptera) of Mexico , 2001 .

[14]  M. L. Goff,et al.  Comparison of patterns of decomposition in a hanging carcass and a carcass in contact with soil in a xerophytic habitat on the Island of Oahu, Hawaii. , 2000, Journal of forensic sciences.

[15]  J. Byrd,et al.  Forensic Entomology: The Utility of Arthropods in Legal Investigations , 2000 .

[16]  M. L. Goff,et al.  Arthropod succession patterns onto burnt carrion in two contrasting habitats in the Hawaiian Islands. , 1998, Journal of forensic sciences.

[17]  M. Moura,et al.  A preliminary analysis of insects of medico-legal importance in Curitiba, State of Paraná. , 1997, Memorias do Instituto Oswaldo Cruz.

[18]  M. Micozzi.  Frozen Environments and Soft Tissue Preservation , 1996 .

[19]  F T Zugibe,et al.  The Iceman murder: one of a series of contract murders. , 1993, Journal of forensic sciences.

[20]  M. L. Goff,et al.  Comparison of insect species associated with decomposing remains recovered inside dwellings and outdoors on the island of Oahu, Hawaii. , 1991, Journal of forensic sciences.

[21]  Kenneth G. V. Smith.  A manual of forensic entomology , 1988 .

[22]  M. L. Goff,et al.  Arthropod succession in exposed carrion in a tropical rainforest on O'ahu Island, Hawai'i. , 1987, Journal of medical entomology.

[23]  M. Micozzi.  Experimental study of postmortem change under field conditions: effects of freezing, thawing, and mechanical injury. , 1986, Journal of forensic sciences.

[24]  R. Putman.  The role of carrion‐frequenting arthropods in the decay process , 1978 .

[25]  J. Payne,et al.  A Summer Carrion Study of the Baby Pig Sus Scrofa Linnaeus , 1965 .

[26]  Sasha C. Voss,et al.  Decomposition and insect succession on cadavers inside a vehicle environment , 2008, Forensic science, medicine, and pathology.

[27]  Ø. Hammer,et al.  PAST: PALEONTOLOGICAL STATISTICAL SOFTWARE PACKAGE FOR EDUCATION AND DATA ANALYSIS , 2001 .

[28]  P. Thyssen,et al.  A checklist of arthropods associated with pig carrion and human corpses in southeastern brazil. , 2000, Memorias do Instituto Oswaldo Cruz.

[29]  G. Anderson,et al.  Insect succession on buried carrion in two biogeoclimatic zones of British Columbia. , 1999, Journal of forensic sciences.

[30]  R W Mann,et al.  Time since death and decomposition of the human body: variables and observations in case and experimental field studies. , 1990, Journal of forensic sciences.

[31]  S. Peck,et al.  TAXONOMY, PHYLOGENY AND BIOGEOGRAPHY OF THE CARRION BEETLES OF LATIN AMERICA (COLEOPTERA: SILPHIDAE) , 1985 .