Comparison of Several Aqueous two Phase Solvent Systems (ATPS) for the Fractionation of Biopolymers by Centrifugal Partition Chromatography (CPC)

Abstract Aqueous Two Phase Solvent Systems {ATPS} have been tested in a Centrifugal Partition Chromatograph (Sanki, Kyoto, Japan), to check for stability, backpressure, noiseless baseline and efficiency. From our experiments we conclude that ATPS are compatible with CPC, in particular the stationary phase is very stable and does not require a high rotational speed to stabilize it (1300 rpm was commonly used); but, the efficiency of the CPC apparatus decreases markedly, due to the high viscosity of ATPS as compared to classical biphasic solvent systems. Based en our results, new partition cartridges have been designed, which minimize the bulk volumes and considerably increase the interface, or favor the mixing step of the two phases by an ad-hoc design of both the inlet ducts and channels.