Spatial scattering Raman spectral characteristics of clenbuterol

Problems with “clenbuterol”, a synthetic β2-adrenergic stimulant that includes clenbuterol, ractopamine, and albuterol, have been increasing in recent years. This article focuses on the detection of albuterol, chemically called 3,6-dihydroxy1-methyl-5-oxo-3,5-dihydro-2H-indolium betaine. It is difficult to directly measure the content of clenbuterol in pork due to its messy and uneven composition, which requires a destructive pre-treatment process that takes time and effort. In this study, a method for subsurface food inspection was presented based on a newly developed line-scan spatially scattering Raman spectroscopy technique. A spatially offset Raman spectroscopy system based on line-scan Raman chemical imaging system was built, which was used to collect spatially offset Raman spectra from the samples. Under the condition of one CCD exposure, the spatially offset Raman spectroscopy system can collect a series of Raman spectra simultaneously in a narrow space interval and a wide offset range. Through data analysis, the rare clenbuterol species in the sample can be determined by the position of characteristic peaks. The system was used to collect salbutamol Raman signal, and the results showed that the characteristic peaks of salbutamol were consistent with the standard characteristic peaks, so it was possible to use the system to detect lean meat in meat.