MODIFIED BINARY HAMMERHEAD RIBOZYMES WITH HIGH CATALYTIC ACTIVITY

A series of binary hammerhead ribozymes was designed and assessed in terms of cleavage activity and nuclease resistance. Enhanced nuclease resistance of binary ribozymes was achieved by incorporation of 2′-modified nucleotides at the selective positions along with addition of 3′-3′-linked thymidine cap. These modified binary ribozymes efficiently cleave 190-nucleotides long MDR1 mRNA fragment and display catalytic activity much higher then respective full-length analogs.

[1]  V. Vlassov,et al.  Binary Hammerhead Ribozymes with High Cleavage Activity , 2004, Nucleosides, nucleotides & nucleic acids.

[2]  V. Vlassov,et al.  Catalytic DNA and RNA for Targeting MDR1 mRNA , 2003, Nucleosides, nucleotides & nucleic acids.

[3]  V. Vlassov,et al.  Site-specific modification of the 5"-terminal fragment of PGY1/MDR1 gene mRNA by reactive conjugates of antisense oligonucleotides , 2003 .

[4]  T. Lockett,et al.  Characterizing ribozyme cleavage reactions. , 1997, Methods in molecular biology.

[5]  A. Karpeisky,et al.  Chemical Modification of Hammerhead Ribozymes , 1995, The Journal of Biological Chemistry.