Controlling the porosity of collagen, gelatin and elastin biomaterials by ultrashort laser pulses

Abstract We report on the structural investigation of self-organized micropores generated in thin gelatin, collagen, and collagen–elastin films after single and multishot irradiation with pulse durations ranging from 30 – 100 fs at 800 nm. We systematically studied the effect of laser parameters: laser energy, number of pulses, and pulse duration on the development of the micropores. This work showed that applying laser pulses at different rates significantly modified the thin film surface. The results clearly revealed that femtosecond laser treatment of thin films of biomaterials: gelatin, collagen and collagen–elastin, results in creation of micro/nanopores with different size of cavity formations. Experimentally, it is demonstrated that it is possible to influence the dimensions of the pore sizes, ranging from 100 nm to 2 μm by tuning the laser parameters. We are currently further exploring the possibility of structuring these biomaterials by applying a time delay between separate pulses. First results from cell culture experiments on laser created surface foam of collagen – elastin were successfully obtained, showing the potential of the method to cultivate cells on superficial porous substrates and the preferable selectivity of the cells to proliferate on the laser modified parts of the biopolymer substrate.

[1]  Stefan Lohfeld,et al.  A method to fabricate small features on scaffolds for tissue engineering via selective laser sintering , 2010 .

[2]  Laurent J. Lewis,et al.  Molecular-dynamics study of ablation of solids under femtosecond laser pulses , 2003 .

[3]  R. Narayan,et al.  Laser micro- and nanofabrication of biomaterials , 2011 .

[4]  Shan Sun,et al.  3D femtosecond laser patterning of collagen for directed cell attachment. , 2005, Biomaterials.

[5]  D. Oxtoby.  Nucleation of First-Order Phase Transitions , 1998 .

[6]  Joydip Kundu,et al.  Biomaterial Scaffold Fabrication Techniques for Potential Tissue Engineering Applications , 2010 .

[7]  C. Dong,et al.  Photophysical mechanisms of collagen modification by 80 MHz femtosecond laser. , 2010, Optics express.

[8]  A.Burdick Jason,et al.  Biomaterials for Tissue Engineering Applications , 2011 .

[9]  Guoping Chen,et al.  Scaffold Design for Tissue Engineering , 2002 .

[10]  Maarten Merkx,et al.  High resolution imaging of collagen organisation and synthesis using a versatile collagen specific probe. , 2007, Journal of structural biology.

[11]  Tae Gwan Park,et al.  Surface engineered and drug releasing pre-fabricated scaffolds for tissue engineering. , 2007, Advanced drug delivery reviews.

[12]  Hae Woon Choi,et al.  Micropatterning and characterization of electrospun poly(ε‐caprolactone)/gelatin nanofiber tissue scaffolds by femtosecond laser ablation for tissue engineering applications , 2011, Biotechnology and bioengineering.

[13]  Robin S. Marjoribanks,et al.  Effects of heat transfer and energy absorption in the ablation of biological tissues by pulsetrain-burst (>100 MHz) ultrafast laser processing , 2006, Photonics North.

[14]  Colleen L Flanagan,et al.  Bone tissue engineering using polycaprolactone scaffolds fabricated via selective laser sintering. , 2005, Biomaterials.

[15]  P. Ma,et al.  Biodegradable polymer scaffolds with well-defined interconnected spherical pore network. , 2001, Tissue engineering.

[16]  Lin Wei,et al.  Annealing effect on photovoltaic performance of CdSe quantum-dots-sensitized TiO 2 nanorod solar cells , 2012 .

[17]  Leonid V. Zhigilei,et al.  Mechanisms of laser ablation from molecular dynamics simulations: dependence on the initial temperature and pulse duration , 1999 .

[18]  E. Sachlos,et al.  Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. , 2003, European cells & materials.

[19]  J. Liu.  Simple technique for measurements of pulsed Gaussian-beam spot sizes. , 1982, Optics letters.

[20]  A. Sionkowska,et al.  Properties and modification of porous 3-D collagen/hydroxyapatite composites. , 2013, International journal of biological macromolecules.

[21]  Serena M Best,et al.  Investigating the morphological, mechanical and degradation properties of scaffolds comprising collagen, gelatin and elastin for use in soft tissue engineering. , 2012, Journal of the mechanical behavior of biomedical materials.

[22]  J. Jansen,et al.  Growth behavior of fibroblasts on microgrooved polystyrene. , 1998, Biomaterials.

[23]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part I. Traditional factors. , 2001, Tissue engineering.

[24]  E Amler,et al.  Biochemical and biophysical aspects of collagen nanostructure in the extracellular matrix. , 2007, Physiological research.

[25]  Jörg Krüger,et al.  Ultrashort pulse laser interaction with dielectrics and polymers , 2004 .

[26]  M. Oujja,et al.  Fabrication of porous biopolymer substrates for cell growth by UV laser: The role of pulse duration , 2012 .

[27]  S. Kundu,et al.  Electrospinning: a fascinating fiber fabrication technique. , 2010, Biotechnology advances.

[28]  Tabatabaei Qomi,et al.  The Design of Scaffolds for Use in Tissue Engineering , 2014 .

[29]  François Berthod,et al.  Collagen-Based Biomaterials for Tissue Engineering Applications , 2010, Materials.

[30]  Boris N. Chichkov,et al.  Three dimensional microstructuring of biopolymers by femtosecond laser irradiation , 2009 .

[31]  X. Liao,et al.  Preparation of porous biodegradable polymer and its nanocomposites by supercritical CO 2 foaming for tissue engineering , 2012 .

[32]  S. Damrongsakkul,et al.  Comparison of gelatin and collagen scaffolds for fibroblast cell culture , 2006 .

[33]  C. Murphy,et al.  Epithelial contact guidance on well-defined micro- and nanostructured substrates , 2003, Journal of Cell Science.

[34]  M. Oujja,et al.  UV, visible and IR laser interaction with gelatine , 2007 .

[35]  Rui L. Reis,et al.  Perspectives on: Supercritical Fluid Technology for 3D Tissue Engineering Scaffold Applications , 2009 .