Use of cluster analysis with monoclonal antibodies for taxonomic differentiation of phytopathogenic fungi and for screening and clustering antibodies

Cluster analysis of ELISA (A405) values resulting from tests using all possible combinations of 43 monoclonal antibodies (raised againstPhytophthora megasperma f.sp.glycinea) and 14 phytopathogenic fungi separated these fungi into taxonomically appropriate clusters. Two distance measurements (Pearson's correlation coefficient and normalized Euclidean distance) and five linkage methods (single, complete, median, centroid, and average) were evaluated. The combination of Pearson's correlation coefficient and complete linkage produced the most “natural” clusters. A modification of the data matrix allowed clustering of the monoclonal antibodies by specificity. Numerically coded ELISA values clustered identically with the orginal data. The taxonomic and evolutionary implications of using monoclonal antibodies specific to carbohydrates are discussed.

[1]  S. C. Johnson.  Hierarchical clustering schemes , 1967, Psychometrika.

[2]  J. Wells,et al.  Identification and detection of Erwinia amylovora with monoclonal antibodies. , 1987 .

[3]  J. Iványi,et al.  ANTIGENIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS AND MYCOBACTERIUM BOVIS DETECTED BY MEANS OF MONOCLONAL ANTIBODIES , 1981, The Lancet.

[4]  A. A. Benedict,et al.  Identification of xanthomonads and grouping of strains of Xanthomonas campestris pv. campestris with monoclonal antibodies , 1985 .

[5]  S. Roth.  Are Glycosyltransferases the Evolutionary Antecedents of the Immunoglobulins? , 1985, The Quarterly Review of Biology.

[6]  Robert R. Sokal,et al.  A statistical method for evaluating systematic relationships , 1958 .

[7]  P. Underwood.  Mapping of antigenic changes in the haemagglutinin of Hong Kong influenza (H3N2) strains using a large panel of monoclonal antibodies. , 1982, The Journal of general virology.

[8]  John A. Hartigan,et al.  Clustering Algorithms , 1975 .

[9]  N. Nathanson,et al.  Characterization of monoclonal antibodies against the G1 and N proteins of LaCrosse and Tahyna, two California serogroup bunyaviruses. , 1982, Virology.

[10]  E. Conway de Macario,et al.  The superficial antigenic mosaic of Methanobrevibacter smithii , 1985, Archives of Microbiology.

[11]  Louis L. McQuitty,et al.  Hierarchical Syndrome Analysis1 , 1960 .

[12]  C. Hickman.  Key to the Species of Phytophthora de Bary, Grace M. Waterhouse, in: Mycological Papers no. 92. Commonwealth Mycological Institute, Kew (1963), 23, sd, . 6 , 1964 .

[13]  R. Sokal,et al.  Principles of numerical taxonomy , 1965 .

[14]  H. Katznelson,et al.  Suppression and stimulation of mycelial growth of Phytophthora cryptogea by certain thiamine-requiring and thiamine-synthesizing bacteria. , 1961, Canadian Journal of Microbiology (print).

[15]  J. Gower.  A comparison of some methods of cluster analysis. , 1967, Biometrics.

[16]  G. Banowetz,et al.  Immunological Comparisons of Teliospores of two Wheat Bunt Fungi, Tilletia Species, Using Monoclonal Antibodies and Antisera , 1984 .

[17]  L. Haley.  Introductory Mycology , 1953, The Yale Journal of Biology and Medicine.

[18]  A. Capron,et al.  Subspecies-specific surface antigens of promastigotes of the Leishmania donovani complex , 1985, Infection and immunity.

[19]  J. David,et al.  Monoclonal antibodies that distinguish between New World species of Leishmania , 1981, Nature.

[20]  P. Tijssen,et al.  Practice and theory of enzyme immunoassays , 1985 .

[21]  D. Roberts,et al.  The statistical program SYSTAT : Wilkinson, L. (1986). SYSTAT, The System for Statistics. SYSTAT, Inc. 2902 Central Street, Evanston, IL 60201. , 1987 .

[22]  A. R. Ayers,et al.  Monoclonal Antibodies to Glycoprotein Antigens of a Fungal Plant Pathogen, Phytophthora megasperma f. sp. glycinea. , 1987, Plant physiology.

[23]  A. Osterhaus,et al.  Analysis of antigenic relationships among influenza virus strains using a taxonomic cluster procedure. Comparison of three kinds of antibody preparations. , 1985, Journal of virological methods.

[24]  G. W. Milligan,et al.  An examination of the effect of six types of error perturbation on fifteen clustering algorithms , 1980 .

[25]  E. Suzaki,et al.  Monoclonal antibodies to isolate-, species-, and genus-specific components on the surface of zoospores and cysts of the fungus Phytophthora cinnamomi. , 1986 .

[26]  J. Tuite.  Plant pathological methods : fungi and bacteria , 1970 .

[27]  O. Kandler,et al.  Six antigenic determinants in the surface layer of the archaebacterium Methanococcus vannielii revealed by monoclonal antibodies. , 1984, Journal of immunology.

[28]  Punjani Mr.  Letter: "Menstrual toxin". , 1974 .