Investigation of the antiadhesive properties of human mesothelial cells cultured in vitro on implantable surgical materials.

The aim of the study was to evaluate the interactions of Permacol, Prolene mesh, Surgisis Gold, and Alloderm with human mesothelial cells in vitro. The capacity of primary human mesothelial cells to adhere to the surface of Alloderm, Surgisis Gold, Prolene mesh, and Permacol as well as support the proliferation and viability of the seeded cells was determined. Production of antifibrinolytic, fibrinolytic, and inflammatory mediators (IL-8, TPA, MMP-1, PAI-1, and TGF-beta) was assessed over an 8-day period. The adhesive nature of the implantable materials was determined by assessment of the strength of any fibrin clots formed between two surfaces of each implant material. Surgisis Gold and Permacol were capable of supporting the attachment and proliferation of primary human mesothelial cells and maintaining viability over an 8-day culture period. Mesothelial cells were shown to have covered the surface of Permacol and Surgisis Gold in a monolayer. The viability of cells cultured on Permacol was significantly greater than the other implant materials tested. Mesothelial cells cultured on Permacol or Surgisis were shown to be producing high levels of fibrinolytic compounds and low levels of antifibrinolytic and inflammatory mediators. Alloderm was shown to produce high levels of IL-8 and antifibrinolytic mediators when compared with the other implantable materials. Permacol was shown to be an unreliable surface for clot formation in vitro and any clots formed were shown to be significantly weaker than the clots produced between two surfaces of tissue culture plastic, Prolene mesh, Alloderm, and Surgisis Gold. This in vitro study indicated that Permacol and Surgisis Gold supported the growth and fibrinolytic activity of human mesothelial cells; however, Permacol was shown to be superior in this respect.

[1]  P. Giordano,et al.  Anterior abdominal wall reconstruction with a Permacol implant. , 2006, Journal of plastic, reconstructive & aesthetic surgery : JPRAS.

[2]  T. Chan,et al.  Peritoneal Mesothelial Cell Culture and Biology , 2006, Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis.

[3]  J. Deprest,et al.  Comparison of host response to polypropylene and non‐cross‐linked porcine small intestine serosal‐derived collagen implants in a rat model , 2005, BJOG : an international journal of obstetrics and gynaecology.

[4]  R. Kaleya.  Evaluation of implant/host tissue interactions following intraperitoneal implantation of porcine dermal collagen prosthesis in the rat , 2005, Hernia.

[5]  S. Mutsaers,et al.  Mesothelial progenitor cells and their potential in tissue engineering. , 2004, The international journal of biochemistry & cell biology.

[6]  S. Mutsaers.  Mesothelial Cell , 2020, Definitions.

[7]  A. Saray.  Porcine Dermal Collagen (Permacol) for Facial Contour Augmentation: Preliminary Report , 2003, Aesthetic Plastic Surgery.

[8]  S. Mutsaers.  Mesothelial cells: Their structure, function and role in serosal repair , 2002, Respirology.

[9]  C. V. van Blitterswijk,et al.  Effect of fibroblasts on epidermal regeneration , 2002, The British journal of dermatology.

[10]  G. Laurent,et al.  Role of plasminogen activators in peritoneal adhesion formation. , 2002, Biochemical Society transactions.

[11]  J. Weisel,et al.  The effect of dimethylbiguanide on thrombin activity, FXIII activation, fibrin polymerization, and fibrin clot formation. , 2002, Diabetes.

[12]  C. Harper,et al.  Permacol: clinical experience with a new biomaterial. , 2001, Hospital medicine.

[13]  R. Foster,et al.  Complex Abdominal Wall Reconstruction: A Comparison of Flap and Mesh Closure , 2000, Annals of surgery.

[14]  E. Steyerberg,et al.  Intraperitoneal polypropylene mesh repair of incisional hernia is not associated with enterocutaneous fistula , 2000, The British journal of surgery.

[15]  P. Amid.  Intraperitoneal polypropylene mesh repair of incisional hernia is not associated with enterocutaneous fistula. , 2000, The British journal of surgery.

[16]  H. Lehr,et al.  Remodeling of peritoneal-like structures by mesothelial cells: its role in peritoneal healing. , 1999, The Journal of surgical research.

[17]  S. Mutsaers,et al.  Cytokine regulation of mesothelial cell proliferation in vitro and in vivo. , 1997, European journal of cell biology.

[18]  J. Pachence,et al.  Collagen-based devices for soft tissue repair. , 1996, Journal of biomedical materials research.

[19]  S. Badylak,et al.  Intestine submucosa and polypropylene mesh for abdominal wall repair in dogs. , 1996, The Journal of surgical research.

[20]  S. Badylak,et al.  Small Intestinal Submucosa: Utilization for Repair of Rodent Abdominal Wall Defects , 1995, Annals of plastic surgery.

[21]  S. Badylak,et al.  The use of xenogeneic small intestinal submucosa as a biomaterial for Achilles tendon repair in a dog model. , 1995, Journal of biomedical materials research.

[22]  D. J. Wainwright.  Use of an acellular allograft dermal matrix (AlloDerm) in the management of full-thickness burns. , 1995, Burns : journal of the International Society for Burn Injuries.

[23]  E. Stylianou,et al.  Isolation, culture and characterization of human peritoneal mesothelial cells. , 1990, Kidney international.

[24]  L A Geddes,et al.  Small intestinal submucosa as a large diameter vascular graft in the dog. , 1989, The Journal of surgical research.

[25]  Holl-Allen Rt.  Porcine dermal collagen implants in man. , 1984 .

[26]  Holl-Allen Rt.  Porcine dermal collagen repair of inguinal hernias. , 1984 .

[27]  R. Holl‐Allen.  Porcine dermal collagen repair of inguinal hernias. , 1984, Journal of the Royal College of Surgeons of Edinburgh.