Abnormal accumulation of sulphated materials in lens tissue of mice with the aphakia mutation.

Sulphated materials were tested for in the eyes of late gestational and postnatal normal mice and mice with the aphakia mutation using Spicer's high iron diamine staining method. Qualitative identification of these materials was attempted with bovine testicular hyaluronidase and nitrous acid digestion methods. The grossly abnormal morphology of the aphakia lens made it necessary to confirm identification of lens-derived tissue by testing for lens crystallins using standard immunohistological methods. As seen in normal mouse lens maturation, accumulated inter- and intracellular sulphated materials were observed in aphakia lens tissue from just before birth through juvenile maturation. Large cyst-like structures consisting of lens-derived tissue were commonly seen in the eyes of young postnatal mutant mice. Sulphated materials formed basal lamina-like structures on many of these lens-derived units, but a well-defined lens capsule never formed. Abnormal fibrillar structures rich in sulphated materials were seen in the intraocular cavity in many older mutant specimens, most of which were largely resistant to both digestion methods. These results indicate that the potential to elaborate sulphated materials qualitatively similar to those seen in normal mouse lens maturation is present in the aphakia mutant, although the mode of accumulation is grossly disturbed.

[1]  J. Zwaan,et al.  Accumulation and distribution of sulfated materials in the maturing mouse lens capsule , 1987, The Anatomical record.

[2]  J. Zwaan,et al.  The appearance of α, β and γ crystallins in an anophthalmic strain of mice , 1984 .

[3]  R. Orkin,et al.  Biosynthesis of sulphated macromolecules by rabbit lens epithelium. I. Identification of the major macromolecules synthesized by lens epithelial cells in vitro , 1984, The Journal of cell biology.

[4]  J. Zwaan,et al.  Histochemical analysis of extracellular matrix material during embryonic mouse lens morphogenesis in an aphakic strain of mice. , 1984, Developmental biology.

[5]  A. Silver,et al.  The extracellular matrix between the optic vesicle and presumptive lens during lens morphogenesis in an anophthalmic strain of mice. , 1984, Developmental biology.

[6]  A. Silver,et al.  Histochemical analysis of extracellular matrix material in embryonic mouse lens morphogenesis. , 1983, Developmental biology.

[7]  D. Solter,et al.  Identification and purification of a cell surface glycoprotein mediating intercellular adhesion in embryonic and adult tissue , 1983, Cell.

[8]  Koniukhov Bv,et al.  Ultrastructural analysis of the effects of the aphakia gene in mice embryos , 1983 .

[9]  J. Silver,et al.  Crystallin synthesis in the lens rudiment of a strain of mice with congenital anophthalmia. , 1983, Experimental eye research.

[10]  Johan Zwaan,et al.  The appearance of α-crystallin in relation to cell cycle phase in the embryonic mouse lens , 1983 .

[11]  J. W. Yang,et al.  The effect of inhibitors of glycoconjugate synthesis on optic cup formation in the chick embryo. , 1982, Developmental biology.

[12]  S. Robert Hilfer,et al.  Accumulation of CPC‐precipitable material at apical cell surfaces during formation of the optic cup , 1980, The Anatomical record.

[13]  B. Spooner,et al.  Development of the embryonic mammalian pancreas: the relationship between morphogenesis and cytodifferentiation. , 1977, Developmental biology.

[14]  J. Zwaan,et al.  Malorientation of mitotic figures in the early lens rudiment of aphakia mouse embryos , 1975, The Anatomical record.

[15]  J. Zwaan.  Immunofluorescent studies on aphakia, a mutation of a gene involved in the control of lens differentiation in the mouse embryo. , 1975, Developmental Biology.

[16]  M. Evans,et al.  Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[17]  R. Kosher,et al.  Sulfated mucopolysaccharide synthesis during the development of Rana pipiens. , 1973, Developmental biology.

[18]  B. Toole,et al.  The extracellular matrix of the regenerating newt limb: synthesis and removal of hyaluronate prior to differentiation. , 1971, Developmental biology.

[19]  D. Cogan,et al.  Electron microscopic study of galactose-induced cataract. , 1969, Investigative ophthalmology.

[20]  D S Varnum,et al.  Aphakia, a new mutation in the mouse. , 1968, The Journal of heredity.

[21]  J. Zwaan,et al.  The changing cellular localization of alpha-crystallin in the lens of the chicken embryo, studied by immunofluorescence. , 1967, Developmental biology.

[22]  S. Spicer.  DIAMINE METHODS FOR DIFFERENTIATING MUCOSUBSTANCES HISTOCHEMICALLY , 1965 .

[23]  A. Cohen.  Electron microscopic observations on the lens of the neonatal albino mouse. , 1958, The American journal of anatomy.

[24]  D. Rytel,et al.  Contribution to the histopathology of cataract. , 1955, A.M.A. archives of ophthalmology.

[25]  A. Patz.  Cataracts in galactosemia; observations in three cases. , 1953, American journal of ophthalmology.

[26]  L. Sallmann.  Experimental studies on early lens changes after roentgen irradiation. I. Morphological and cytochemical changes. , 1951 .

[27]  J Zwaan,et al.  The appearance of alpha-crystallin in relation to cell cycle phase in the embryonic mouse lens. , 1983, Developmental biology.

[28]  Robert J. Wordinger,et al.  Manual of immunoperoxidase techniques , 1983 .

[29]  L. von Sallmann.  Experimental studies on early lens changes after roentgen irradiation. I. Morphological and cytochemical changes. , 1951, A.M.A. archives of ophthalmology.