Protective Effect of Carrageenan against Murine Cytomegalovirus Infection in Mice

The protective effect of iota-carrageenan (CAR) was evaluated against murine cytomegalovirus (MCMV) infection in mice. Female ICR mice were challenged intraperitoneally (i.p.) with 3 LD50 of salivary gland-passaged MCMV. More than 0.5 mg of CAR showed a protective effect on mice only when CAR was administered i.p. and then MCMV was inoculated i.p. The protective effect of CAR was evidenced by an increase in plaque-forming unit per LD50 and a decrease in the titre of infectious viruses in the target organs. Neither a virucidal nor a virustatic effect on MCMV was evidenced for CAR. The protective effect of CAR seemed to be host-mediated. Pretreatment of mice with CAR augmented natural killer (NK) activity of the spleen cells without elevating the serum interferon level. However, administration of anti-asialo GM1 antibody did not nullify the inhibitory effect of CAR on virus replication in the target organs. MCMV infection induced leukopenia including neutropenia and lymphopenia in saline-treated mice. Pretreatment with CAR protected mice from those signs, except for slight lymphopenia. Administration of cyclophosphamide induced severe leukopenia including neutropenia and lymphopenia even in CAR-treated mice. Under such conditions, the protective effect of CAR against MCMV infection was abrogated by cyclophosphamide. Thus, the protective effect of CAR seems to be non-NK-mediated.

[1]  Y. Minamishima,et al.  Protective effect of MDP-Lys(L18), a synthetic derivative of muramyldipeptide, on murine cytomegalovirus infection. , 1992, Natural immunity.

[2]  A. Adam,et al.  Kinins and peritoneal exudates induced by carrageenin and zymosan in rats , 1990, British journal of pharmacology.

[3]  J. Shanley.  In vivo administration of monoclonal antibody to the NK 1.1 antigen of natural killer cells: Effect on acute murine cytomegalovirus infection , 1990, Journal of medical virology.

[4]  S. Nicklin,et al.  Intestinal uptake and immunological effects of carrageenan--current concepts. , 1989, Food additives and contaminants.

[5]  Y. Minamishima,et al.  Host-mediated antiviral activity ofLactobacillus casei against cytomegalovirus infection in mice , 1989, Biotherapy.

[6]  M. Kurzer,et al.  Indomethacin decreases carrageenan-induced peritoneal adhesions. , 1988, Surgery.

[7]  H. Nakashima,et al.  Purification and characterization of an avian myeloblastosis and human immunodeficiency virus reverse transcriptase inhibitor, sulfated polysaccharides extracted from sea algae , 1987, Antimicrobial Agents and Chemotherapy.

[8]  María Eugenia González,et al.  Polysaccharides as Antiviral Agents: Antiviral Activity of Carrageenan , 1987, Antimicrobial Agents and Chemotherapy.

[9]  M. Okada,et al.  The Effect of Biological Response Modifiers on Chronic and Latent Murine Cytomegalovirus Infections , 1987, Microbiology and immunology.

[10]  K. Nomoto,et al.  Mechanism of protection during the early phase of a generalized viral infection. II. Contribution of polymorphonuclear leukocytes to protection against intravenous infection with influenza virus. , 1987, The Journal of general virology.

[11]  Y. Minamishima,et al.  The Efficacy of Biological Response Modifiers against Murine Cytomegalovirus Infection in Normal and Immunodeficient Mice , 1987, Microbiology and immunology.

[12]  R I Lehrer,et al.  Direct inactivation of viruses by human granulocyte defensins , 1986, Journal of virology.

[13]  J. Murphy,et al.  Correlation of natural killer cell activity and clearance of Cryptococcus neoformans from mice after adoptive transfer of splenic nylon wool-nonadherent cells , 1986, Infection and immunity.

[14]  T. Greiner,et al.  The Interaction of Murine Cytomegalovirus With Murine Neutrophils: Effect on Migratory and Phagocytic Activities , 1985, Journal of leukocyte biology.

[15]  Luis Carrasco,et al.  Screening for new compounds with antiherpes activity. , 1984, Antiviral research.

[16]  B. Woda,et al.  Pathogenesis of murine cytomegalovirus infection in natural killer cell-depleted mice , 1984, Journal of virology.

[17]  Y. Minamishima,et al.  Protective effect of biological response modifiers on murine cytomegalovirus infection , 1984, Journal of virology.

[18]  I. Gresser,et al.  Interferon as a defence mechanism in mouse cytomegalovirus infection. , 1983, The Journal of general virology.

[19]  J. Truax,et al.  Pathway of onset, development, and decay of carrageenan pleurisy in the rat. , 1982, Federation proceedings.

[20]  J. Trapman,et al.  Evidence for a protective role of interferon in resistance to murine cytomegalovirus and its control by non-H-2-linked genes , 1982, Infection and immunity.

[21]  G. Bancroft,et al.  Increased susceptibility to cytomegalovirus infection in beige mutant mice. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[22]  P. Hochman,et al.  Carrageenan-induced decline of natural killer activity. II. Inhibition of cytolysis by adherent non-T La-negative suppressor cells activated in vivo. , 1981, Cellular immunology.

[23]  C. Riccardi,et al.  Transfer to cyclophosphamide-treated mice of natural killer (NK) cells and in vivo natural reactivity against tumors. , 1981, Journal of immunology.

[24]  P. Quan,et al.  Modifications of the immune system in carrageenan-treated mice. , 1981, Journal of the Reticuloendothelial Society.

[25]  G. Bancroft,et al.  Genetic influences on the augmentation of natural killer (NK) cells during murine cytomegalovirus infection: correlation with patterns of resistance. , 1981, Journal of immunology.

[26]  G. Quinnan,,et al.  Antivirus antibody-dependent cell-mediated cytotoxicity during murine cytomegalovirus infection , 1980, Infection and immunity.

[27]  P. Quan,et al.  NK activity in carrageenan-treated mice. , 1980, Immunology.

[28]  G. Quinnan,,et al.  Role of cytotoxic T lymphocytes in murine cytomegalovirus infection. , 1980, The Journal of general virology.

[29]  A. Allison,et al.  Role of T lymphocytes in recovery from murine cytomegalovirus infection , 1977, Infection and immunity.

[30]  M. Selgrade,et al.  Role of Macrophages in Resistance to Murine Cytomegalovirus , 1974, Infection and immunity.

[31]  R. Graham,et al.  Spectrum and possible mechanism of carrageenan cytotoxicity. , 1971, The American journal of pathology.

[32]  A. Goldin,et al.  Hematopoietic recovery after large doses of cyclophosphamide: correlation of proliferative state with sensitivity. , 1970, Cancer research.

[33]  W. Fried,et al.  The effect of cyclophosphamide on hematopoietic stem cells. , 1968, Radiation research.

[34]  J. Osborn,et al.  Immunosuppression during acute murine cytomegalovirus infection. , 1968, Journal of immunology.

[35]  Y. Minamishima,et al.  Effect of Chlorella vulgaris extracts on murine cytomegalovirus infections. , 1990, Natural immunity and cell growth regulation.

[36]  Y. Minamishima,et al.  Induction of resistance in mice against murine cytomegalovirus by cellular components ofLactobacillus casei , 1989, Biotherapy.

[37]  大橋 剛.  Host-mediated antiviral activity of Lactobacillus casei against cytomegalovirus infection in mice , 1988 .

[38]  J. Truax,et al.  Pathway to carrageenan-induced inflammation in the hind limb of the rat. , 1987, Federation proceedings.

[39]  S. Adler,et al.  Effects of chemotherapeutic agents on hemopoietic stromal tissue: I. Effects of cyclophosphamide and bleomycin on hemopoiesis in subcutaneous femur implants. , 1984, Experimental hematology.