论文信息 - Coimmobilization of urease and glutamate dehydrogenase in electrochemically prepared polypyrrole-polyvinyl sulfonate films

Coimmobilization of urease and glutamate dehydrogenase in electrochemically prepared polypyrrole-polyvinyl sulfonate films

Immobilization of urease and glutamate dehydrogenase enzymes in electrochemically prepared polypyrrole-polyvinyl sulfonate films (PPY-PVS) was carried out using physical adsorption and electrochemical entrapment techniques. Detailed studies on optimum pH, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy of the enzymes in the immobilized state were conducted. The value of the apparent Michaelis-Menten constant was experimentally determined to be 2.5 and 2.7 for physically adsorbed and electrochemically entrapped urease in PPY-PVS films, respectively.

[1] J Wang,et al. Processible polyaniline as an advanced potentiometric pH transducer. Application to biosensors. , 1999, Analytical chemistry.

[2] Bansi D. Malhotra,et al. ENHANCED LOADING OF GLUCOSE OXIDASE ON POLYANILINE FILMS BASED ON ANION EXCHANGE , 1998 .

[3] V. Olazábal,et al. Electrogeneration of polypyrrole in presence of polyvinylsulphonate. Kinetic study , 1996 .

[4] Bansi D. Malhotra,et al. Immobilization of Lactate Dehydrogenase on Electrochemically Prepared Polyaniline Films , 1999 .

[5] Bansi D. Malhotra,et al. Application of polyaniline-Langmuir-Blodgett films as a glucose biosensor , 1995 .

[6] Hak-sung Kim,et al. Electrochemical characterization of polypyrrole/glucose oxidase biosensor: Part I. Influence of enzyme concentration on the growth and properties of the film , 1996 .

[7] Philip N. Bartlett,et al. Electrochemical immobilisation of enzymes: Part II. Glucose oxidase immobilised in poly-N-methylpyrrole , 1987 .

[8] D. Pletcher,et al. The electrodeposition of polypyrrole films from aqueous solutions , 1984 .

[9] Prem C. Pandey,et al. Conducting polymer-coated enzyme microsensor for urea , 1988 .

[10] L. Goldstein. Microenvironmental effects on enzyme catalysis. A kinetic study of polyanionic and polycationic derivatives of chymotrypsin. , 1972, Biochemistry.

[11] G. Guilbault,et al. A urea-specific enzyme electrode. , 1969, Journal of the American Chemical Society.