Real-time online in situ monitoring and statistical design strategies for haematopoietic stem cell bioprocessing

......................................................................... 3 Acknowledgements ................................................................. 4 Publications .......................................................................... 6

[1]  D. Slamon,et al.  Culture conditions affect the ability of ex vivo expanded peripheral blood progenitor cells to accelerate hematopoietic recovery. , 2002, Experimental hematology.

[2]  R. Lemieux,et al.  Increased megakaryopoiesis in cultures of CD34‐enriched cord blood cells maintained at 39°C , 2004 .

[3]  Guo-Li Shen,et al.  Amperometric glucose biosensor based on chitosan with improved selectivity and stability , 2004 .

[4]  H. Mayani,et al.  In vitro proliferation, expansion, and differentiation of a CD34+ cell-enriched hematopoietic cell population from human umbilical cord blood in response to recombinant cytokines. , 2002, Archives of medical research.

[5]  B. Löwenberg,et al.  Acute myeloid leukemia and the position of autologous stem cell transplantation. , 2007, Seminars in hematology.

[6]  Huaiguo Xue,et al.  Improved selectivity and stability of glucose biosensor based on in situ electropolymerized polyaniline-polyacrylonitrile composite film. , 2005, Biosensors & bioelectronics.

[7]  J. Wingard,et al.  Human cord blood long-term engrafting cells are CD34+ CD38− , 2003, Leukemia.

[8]  Y. Sonoda,et al.  Identification and Hematopoietic Potential of CD45− Clonal Cells with Very Immature Phenotype (CD45−CD34−CD38−Lin−) in Patients with Myelodysplastic Syndromes , 2005, Stem cells.

[9]  Gabriela Canziani,et al.  Exploring biomolecular recognition using optical biosensors. , 1999, Methods.

[10]  Douglas C. Montgomery,et al.  Response Surface Methodology: Process and Product Optimization Using Designed Experiments , 1995 .

[11]  C. Civin,et al.  Flow cytometric analysis of human bone marrow: I. Normal erythroid development. , 1987, Blood.

[12]  J. Dick,et al.  Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[13]  A. Skottner,et al.  Comparative studies of the erythroid-potentiating effects of biosynthetic human insulin-like growth factors-I and -II. , 1992, The Journal of clinical endocrinology and metabolism.

[14]  Amine Kamen,et al.  A flow-injection (FI) mediated biosensor for on-line monitoring of lactate in mammalian cell culture , 1997 .

[15]  T. Nakahata,et al.  Ex vivo expansion of hematopoietic stem cells by cytokines. , 2002, Biochimica et biophysica acta.

[16]  L. Douay,et al.  Ex vivo production of human red blood cells from hematopoietic stem cells: what is the future in transfusion? , 2007, Transfusion medicine reviews.

[17]  J. Guez,et al.  Real time in situ microscopy for animal cell-concentration monitoring during high density culture in bioreactor. , 2004, Journal of biotechnology.

[18]  M. Cairo,et al.  Cord blood immunology and stem cell transplantation. , 2005, Human immunology.

[19]  M. Bernard,et al.  Patients suffering from acute graft-versus-host disease after bone-marrow transplantation have functional CD4+CD25hiFoxp3+ regulatory T cells. , 2008, Clinical immunology.

[20]  D F Marks,et al.  Extended culture of multipotent hematopoietic progenitors without cytokine augmentation in a novel three-dimensional device. , 1999, Experimental hematology.

[21]  Bonnie C. Baker,et al.  Precision Temperature-Sensing With RTD Circuits , 2003 .

[22]  R D Schmid,et al.  On-line determination of glucose concentration throughout animal cell cultures based on chemiluminescent detection of hydrogen peroxide coupled with flow-injection analysis. , 1991, Journal of biotechnology.

[23]  J D Newman,et al.  Biosensors for process control. , 1991, Enzyme and microbial technology.

[24]  C. Ng,et al.  Gel‐entrapment of perfluorocarbons: A fluorine‐19 NMR spectroscopic method for monitoring oxygen concentration in cell perfusion systems , 1993, Magnetic resonance in medicine.

[25]  L. Kaplan,et al.  Why Should Clinicians Be Concerned about Blood Conservation ? , 2005 .

[26]  Thomas Scheper,et al.  In-situ microscopy: Online process monitoring of mammalian cell cultures , 2004, Cytotechnology.

[27]  D. Blaise,et al.  [Hematopoietic growth factors]. , 1993, Presse medicale.

[28]  Mike Hoare,et al.  Rapid monitoring of recombinant protein products: a comparison of current technologies. , 2002, Trends in biotechnology.

[29]  Henri Wajcman,et al.  Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo , 2002, Nature Biotechnology.

[30]  Marcel Ottens,et al.  Lab‐scale fermentation tests of microchip with integrated electrochemical sensors for pH, temperature, dissolved oxygen and viable biomass concentration , 2008, Biotechnology and bioengineering.

[31]  Athanasios Mantalaris,et al.  A combined fluid dynamics, mass transport and cell growth model for a three-dimensional perfused biorector for tissue engineering of haematopoietic cells , 2007 .

[32]  P. Reddy,et al.  GVHD pathophysiology: is acute different from chronic? , 2008, Best practice & research. Clinical haematology.

[33]  F. Kovářů,et al.  Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining. , 2007, Journal of immunological methods.

[34]  Viswanath Devanarayan,et al.  Development, validation, and implementation of a multiplex immunoassay for the simultaneous determination of five cytokines in human serum. , 2005, Journal of pharmaceutical and biomedical analysis.

[35]  Casey K. Chan,et al.  Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells. , 2008, Biomaterials.

[36]  Hiroyuki Sakatoku,et al.  In Vitro Proliferation and Differentiation of Erythroid Progenitors of Cord Blood , 1997, Stem cells.

[37]  M. Martinez-Moczygemba,et al.  Biology of common beta receptor-signaling cytokines: IL-3, IL-5, and GM-CSF. , 2003, The Journal of allergy and clinical immunology.

[38]  K F Reardon,et al.  Immuno- and flow cytometric analytical methods for biotechnological research and process monitoring. , 1992, Journal of biotechnology.

[39]  S. Marley,et al.  Potential of CD34 in the Regulation of Symmetrical and Asymmetrical Divisions by Hematopoietic Progenitor Cells , 2007, Stem cells.

[40]  W M Miller,et al.  Higher pH Promotes Megakaryocytic Maturation and Apoptosis , 2002, Stem cells.

[41]  Bernhard Sonnleitner,et al.  Automatic bioprocess control. 3. Impacts on process perception , 1991 .

[42]  S. Ramakrishna,et al.  Functional nanofiber scaffolds with different spacers modulate adhesion and expansion of cryopreserved umbilical cord blood hematopoietic stem/progenitor cells. , 2007, Experimental hematology.

[43]  I-Ming Chu,et al.  Factorial designs combined with the steepest ascent method to optimize serum-free media for ex vivo expansion of human hematopoietic progenitor cells , 2003 .

[44]  W M Miller,et al.  Oxygen tension modulates the expression of cytokine receptors, transcription factors, and lineage-specific markers in cultured human megakaryocytes. , 2001, Experimental hematology.

[45]  W. Arcese,et al.  The Therapeutic Use of Cord Blood , 2006 .

[46]  Laurent Kiger,et al.  Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells , 2005, Nature Biotechnology.

[47]  E. Papoutsakis,et al.  pH is a potent modulator of erythroid differentiation , 1998, British journal of haematology.

[48]  V. Stove,et al.  Tumor necrosis factor promotes T-cell at the expense of B-cell lymphoid development from cultured human CD34+ cord blood cells. , 2007, Experimental hematology.

[49]  J. Jansen,et al.  Biocompatibility evaluation of sol-gel coatings for subcutaneously implantable glucose sensors. , 2000, Biomaterials.

[50]  Giuseppe Puglisi,et al.  Long‐term expansion and maintenance of cord blood haematopoietic stem cells using thrombopoietin, Flt3‐ligand, interleukin (IL)‐6 and IL‐11 in a serum‐free and stroma‐free culture system , 2001, British journal of haematology.

[51]  K. Leong,et al.  Expansion of engrafting human hematopoietic stem/progenitor cells in three-dimensional scaffolds with surface-immobilized fibronectin. , 2006, Journal of biomedical materials research. Part A.

[52]  K. Anderson,et al.  Hematologic Complications and Blood Bank Support , 2003 .

[53]  A Nagy,et al.  Stem cell bioengineering. , 2001, Annual review of biomedical engineering.

[54]  Cláudia Lobato da Silva,et al.  Hematopoietic stem cells: from the bone to the bioreactor. , 2003, Trends in biotechnology.

[55]  S. C. Li,et al.  Development of a new radioimmunoassay for erythropoietin using recombinant erythropoietin. , 1990, Kidney international.

[56]  Tiago G Fernandes,et al.  Mouse embryonic stem cell expansion in a microcarrier-based stirred culture system. , 2007, Journal of biotechnology.

[57]  Brian McNeil,et al.  In‐situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation , 2003, Biotechnology and bioengineering.

[58]  J Wagner,et al.  Umbilical cord blood transplantation: current practice and future innovations. , 2003, Critical reviews in oncology/hematology.

[59]  Mutsumi Takagi,et al.  Analysis of hematopoietic microenvironment containing spatial development of stromal cells in nonwoven fabrics. , 2003, Journal of bioscience and bioengineering.

[60]  T. Soma,et al.  3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics. , 2002, Cytotherapy.

[61]  D. J. Harrison,et al.  A Miniaturized Nafion-Based Glucose Sensor: in vitro and in vivo evaluation in dogs , 1994, The International journal of artificial organs.

[62]  A. Zander,et al.  Ex vivo expansion of megakaryocyte progenitors from cryopreserved umbilical cord blood. A potential source of megakaryocytes for transplantation. , 2002, Experimental hematology.

[63]  P. Sathyanarayana,et al.  Erythropoietin-dependent erythropoiesis: New insights and questions. , 2006, Blood cells, molecules & diseases.

[64]  Ioan Notingher,et al.  In situ spectroscopic study of nucleic acids in differentiating embryonic stem cells , 2004 .

[65]  K. Detmer,et al.  Bone morphogenetic proteins act synergistically with haematopoietic cytokines in the differentiation of haematopoietic progenitors. , 2002, Cytokine.

[66]  N. Ende,et al.  Pooled umbilical cord blood as a possible universal donor for marrow reconstitution and use in nuclear accidents. , 2001, Life sciences.

[67]  Alves,et al.  Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells. , 2000, Enzyme and microbial technology.

[68]  Peter W Zandstra,et al.  Synergy between erythropoietin and stem cell factor during erythropoiesis can be quantitatively described without co‐signaling effects , 2008, Biotechnology and bioengineering.

[69]  Emmanuel M. Drakakis,et al.  Towards information-rich bioprocessing: Generation of spatio-temporal profiles through the use of design of experiments to determine optimal number and location of sensors—An example in thermal profiles , 2008 .

[70]  M. Zenke,et al.  Growth and differentiation of human stem cell factor/erythropoietin-dependent erythroid progenitor cells in vitro. , 1998, Blood.

[71]  Martin Gawlitzek,et al.  Chemometric evaluation of on‐line high‐pressure liquid chromatography in mammalian cell cultures: Analysis of amino acids and glucose , 2002, Biotechnology and bioengineering.

[72]  Angela Lin,et al.  Quantitative microcomputed tomography analysis of mineralization within three-dimensional scaffolds in vitro. , 2004, Journal of biomedical materials research. Part A.

[73]  I-Ming Chu,et al.  A systematic strategy to optimize ex vivo expansion medium for human hematopoietic stem cells derived from umbilical cord blood mononuclear cells. , 2004, Experimental hematology.

[74]  Melody M. Anderson,et al.  Long‐term continuous monitoring of dissolved oxygen in cell culture medium for perfused bioreactors using optical oxygen sensors , 2004, Biotechnology and bioengineering.

[75]  Bernard Gallez,et al.  Assessment of tumor oxygenation by electron paramagnetic resonance: principles and applications , 2004, NMR in biomedicine.

[76]  G. Vunjak‐Novakovic,et al.  Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. , 2004, Journal of biomedical materials research. Part A.

[77]  K. Ando,et al.  Murine Stromal Cell Line HESS‐5 Maintains Reconstituting Ability of Ex Vivo‐Generated Hematopoietic Stem Cells from Human Bone Marrow and Cytokine‐Mobilized Peripheral Blood , 2000, Stem Cells.

[78]  M. Malmqvist,et al.  Surface plasmon resonance for detection and measurement of antibody-antigen affinity and kinetics. , 1993, Current opinion in immunology.

[79]  J. Harousseau.  Hematopoietic stem cell transplantation in multiple myeloma. , 2009, Journal of the National Comprehensive Cancer Network : JNCCN.

[80]  K. Brindle,et al.  Mapping of oxygen tension and cell distribution in a hollow-fiber bioreactor using magnetic resonance imaging. , 1997, Biotechnology and bioengineering.

[81]  J H Luong,et al.  On-line monitoring of glucose in mammalian cell culture using a flow injection analysis (FIA) mediated biosensor. , 1997, Biotechnology and bioengineering.

[82]  I. Chaiken,et al.  Biosensor analysis of dynamics of interleukin 5 receptor subunit beta(c) interaction with IL5:IL5R(alpha) complexes. , 2002, Analytical biochemistry.

[83]  W M Miller,et al.  Characterization of Hematopoietic Cell Expansion, Oxygen Uptake, and Glycolysis in a Controlled, Stirred‐Tank Bioreactor System , 1998, Biotechnology progress.

[84]  B. Deng,et al.  Modulation of haematopoietic progenitor development by FLT-3 ligand. , 1999, Cytokine.

[85]  Gerhard J. Mohr,et al.  Optical sensors for a wide pH range based on azo dyes immobilized on a novel support , 1994 .

[86]  John H. T. Luong,et al.  Novel FIA amperometric biosensor system for the determination of glutamine in cell culture systems , 1993 .

[87]  E. Papoutsakis,et al.  Ex vivo culture systems for hematopoietic cells. , 1996, Current opinion in biotechnology.

[88]  A. Nagler,et al.  Umbilical cord blood transplantation--how, when and for whom? , 2004, Blood reviews.

[89]  Toshiomi Yoshida,et al.  Cell processing engineering for ex vivo expansion of hematopoietic cells: a review , 2004 .

[90]  P. Flute,et al.  The examination of the blood. , 1960, The Practitioner.

[91]  Mark A Arnold,et al.  On‐line monitoring of human prostate cancer cells in a perfusion rotating wall vessel by near‐infrared spectroscopy , 2004, Biotechnology and bioengineering.

[92]  David R. Walt,et al.  Simultaneous monitoring of pH, CO2 and O2 using an optical imaging fiber , 1997 .

[93]  I Klimant,et al.  Optical triple sensor for measuring pH, oxygen and carbon dioxide. , 1994, Journal of biotechnology.

[94]  Chris Mason,et al.  Regenerative medicine bioprocessing: building a conceptual framework based on early studies. , 2006, Tissue engineering.

[95]  John H T Luong,et al.  Biosensor technology: technology push versus market pull. , 2008, Biotechnology advances.

[96]  A. Frias,et al.  Generation of functional natural killer and dendritic cells in a human stromal-based serum-free culture system designed for cord blood expansion. , 2008, Experimental hematology.

[97]  C. Zupke,et al.  Nuclear magnetic resonance analysis of cell metabolism. , 1995, Current opinion in biotechnology.

[98]  B. Alberts,et al.  Histology: The Lives and Deaths of Cells in Tissues , 2002 .

[99]  H. Kimura,et al.  Cell-Based Microfluidic Biochip for Electrochemical Real-Time Monitoring of Glucose and Oxygen , 2007, TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference.

[100]  Gerard L. Coté,et al.  Investigation of Near-Infrared Spectroscopy for Periodic Determination of Glucose in Cell Culture Media in Situ , 2000 .

[101]  P. Bourne,et al.  Engineering a human bone marrow model: a case study on ex vivo erythropoiesis. , 1998, Biotechnology progress.

[102]  M. Magócsi,et al.  Signalling mechanisms in erythropoiesis: the enigmatic role of calcium. , 1997, Cellular signalling.

[103]  P S R K Sastry,et al.  Metabolic rate determines haematopoietic stem cell self-renewal. , 2004, Medical hypotheses.

[104]  S. Hou,et al.  Amperometric glucose biosensor based on layer-by-layer assembly of multilayer films composed of chitosan, gold nanoparticles and glucose oxidase modified Pt electrode. , 2007, Biosensors & bioelectronics.

[105]  Melody M. Anderson,et al.  Continuous pH monitoring in a perfused bioreactor system using an optical pH sensor. , 2002, Biotechnology and bioengineering.

[106]  G. Almeida-Porada,et al.  Kinetic Analysis of the ex vivo Expansion of Human Hematopoietic Stem/Progenitor Cells , 2006, Biotechnology Letters.

[107]  Kazuhiro Sudo,et al.  Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells , 2006, Nature Biotechnology.

[108]  Abel L. Thangawng,et al.  An ultra-thin PDMS membrane as a bio/micro–nano interface: fabrication and characterization , 2007, Biomedical microdevices.

[109]  S. Arora,et al.  Analysis of intracellular cytokines using flowcytometry. , 2002, Methods in cell science : an official journal of the Society for In Vitro Biology.

[110]  M. Nostro,et al.  Notch Signaling Induces Apoptosis in Primary Human CD34+ Hematopoietic Progenitor Cells , 2007, Stem cells.

[111]  J. Husband.  Textbook of Medical Physiology. W. B. Saunders Company, Philadelphia, London, Toronto, 1133 pages, approx. 900 figures and diagrams. Price £18.00. , 1978 .

[112]  Henk Lingeman,et al.  Sampling and analytical strategies in on-line bioprocess monitoring and control , 1996 .

[113]  Emmanuel M. Drakakis,et al.  A Real-Time Multi-Channel Monitoring System for Stem Cell Culture Process , 2008, IEEE Transactions on Biomedical Circuits and Systems.

[114]  Yang Liu,et al.  Ex vivo expansion of hematopoietic stem cells derived from umbilical cord blood in rotating wall vessel. , 2006, Journal of biotechnology.

[115]  Richard O. Lynch,et al.  Introduction to Design and Analysis of Experiments , 1999, Technometrics.

[116]  Michael J. McShane,et al.  Near-Infrared Spectroscopy for Determination of Glucose, Lactate, and Ammonia in Cell Culture Media , 1998 .

[117]  S. Emerson,et al.  Ex vivo expansion of hematopoietic cells from umbilical cord blood for clinical transplantation , 1998, Journal of leukocyte biology.

[118]  Jae-Won Park,et al.  Erythropoietin-Independent and -Dependent Stages during in vitro Erythropoiesis , 2007, Acta Haematologica.

[119]  G. Gilmore,et al.  Ex vivo expansion of human umbilical cord blood and peripheral blood CD34(+) hematopoietic stem cells. , 2000, Experimental hematology.

[120]  I McNiece,et al.  Ex vivo expansion of hematopoietic progenitor cells and mature cells. , 2001, Experimental hematology.

[121]  R D Schmid,et al.  On-line determination of glucose and lactate concentrations in animal cell culture based on fibre optic detection of oxygen in flow-injection analysis. , 1992, Biosensors & bioelectronics.

[122]  S. Ikehara,et al.  SCID-repopulating cell activity of human cord blood-derived CD34- cells assured by intra-bone marrow injection. , 2003, Blood.

[123]  Alain Garnier,et al.  Efficient in vitro megakaryocyte maturation using cytokine cocktails optimized by statistical experimental design. , 2005, Experimental hematology.

[124]  Daniel G. Bracewell,et al.  An In-Line Flow Injection Optical Biosensor for Real-Time Bioprocess Monitoring , 2002 .

[125]  S S Ozturk,et al.  Real-time monitoring and control of glucose and lactate concentrations in a mammalian cell perfusion reactor. , 1997, Biotechnology and bioengineering.

[126]  W. Miller,et al.  Evaluation of Cytokines for Expansion of the Megakaryocyte and Granulocyte Lineages , 1997, Stem cells.

[127]  C Herwig,et al.  Know-how and know-why in biochemical engineering. , 2003, Biotechnology advances.

[128]  S. Watt,et al.  Stem cell medicine: umbilical cord blood and its stem cell potential. , 2005, Seminars in fetal & neonatal medicine.

[129]  J M Piret,et al.  Cytokine manipulation of primitive human hematopoietic cell self-renewal. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[130]  V. Maino,et al.  Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression. , 1998, Cytometry.

[131]  F Adebodun,et al.  31P NMR characterization of cellular metabolism during dexamethasone induced apoptosis in human leukemic cell lines , 1994, Journal of cellular physiology.

[132]  Cindy L. Miller,et al.  Common and distinct features of cytokine effects on hematopoietic stem and progenitor cells revealed by dose–response surface analysis , 2002, Biotechnology and bioengineering.

[133]  Yongli Mi,et al.  Micromolding of PDMS scaffolds and microwells for tissue culture and cell patterning: A new method of microfabrication by the self-assembled micropatterns of diblock copolymer micelles , 2006 .

[134]  P. Kuchel,et al.  Elevated glutamate dehydrogenase flux in glucose-deprived hybridoma and myeloma cells: evidence from 1H/15N NMR. , 1998, Biotechnology and bioengineering.

[135]  Christofer Toumazou,et al.  Biocompatible ion selective electrode for monitoring metabolic activity during the growth and cultivation of human cells. , 2008, Biosensors & bioelectronics.

[136]  M Aglietta,et al.  Ex vivo expansion of megakaryocytes. , 2000, Transfusion science.