Factors affecting germination of oospores of Phytophthora infestans

When oospores from the pairing between A 1 and A 2 mating types of Phytophthora infestans were treated with 0.25% KMnO 4 solution for 15 min and incubated at 19 o C under light on a modified S+L medium, germination commenced within 4 days and reached about 70% after 20 days. Under these conditions, more than 25% of oospores obtained from a 4-day-old culture germinated. To obtain a high germination rate of P. infestans oospores, light was essential during germination but not during growth and oospore formation. The optimum time for activation of oospores with 0.25% KMnO 4 was 15 to 30 min and a suitable concentration of KMnO 4 for 15 min activation was 0.25 to 0.45%

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