Chlorpyrifos and deltamethrin degradation potentials of two Lactobacillus plantarum (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae) strains

Bircok toprak bakterisi chlorpyrifos ve deltamethrin gibi sentetik insektisitleri esteraz enzimleriyle ve/veya bunlari karbon ve enerji kaynagi olarak kullanarak parcalayabilmektedir. Bizim bu calismadaki hipotezimiz gida fermantasyonu asamalarinda kullanilan  Lactobacillus plantarum  (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae)’un benzer bir insektisit parcalama potansiyelinin gosterilmesidir. Bu calisma,raroa ei  Lactobacillus plantarum ’un iki farkli susunun (LB-1 ve LB-2) asilamadan sonraki 4 gun icinde, iki farkli insektisit parcalama mekanizmasini gostermek amaciyla 2017-2018 yillarinda, Bursa Uludag Universitesi laboratuvarlarinda, in-vitro kosullarda gerceklestirilmistir. Herhangi bir karbon ve enerji kaynagi icermeyen MS ortami ile karsilastirildiginda, chlorpyrifos ve deltamethrin iceren MS ortaminda onemli duzeyde LB-1 gelisimi saptanmistir. Ayrica, bu sus icin onemli duzeyde artan hidroliz aktivitesi de gozlemlenmistir. Bu ozellikler LB-2’de bir miktar daha dusuk bulunmustur. GC-MS cihazi ile yapilan periyodik analizler sonucunda, LB-1 ve LB-2 inokule edilmis MS ortami icinde chlorpyrifos ve deltamethrin’in parcalama oranlari, 3 gun sonra chlorpyrifos icin sirasiyla %96 ve 90, deltamethrin icin %24 ve 53 olarak belirlenmistir. Deltamethrin icin onemli duzeyde bir parcalanma (%86-82) inkubasyondan 10 gun sonra gerceklesmistir. Bu calisma, denemede kullanilan  L. plantarum  suslarinin chlorpyrifos ve deltamethrin parcalama potansiyellerinin oldugunu gostermistir. Ileride bazi meyve sebzelerin fermentasyon sureclerinde kullanimi ve bu suslarin farkli inokulasyon oranlarinda etkinliginin belirlenmesi amaciyla daha fazla calisma yapilmasi gerekmektedir.

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