Enhancement of Polyphenol Content and Antioxidant Activity of Brown Alga Eisenia bicyclis Extract by Microbial Fermentation

The objective of this study was to select an effective microbial strain to improve the functional qualities of Eisenia bicyclis water extract by fermentation. For this purpose, several microorganisms isolated from traditional Korean fermented foods were inoculated and cultivated in E. bicyclis water extract. Ultimately, yeast strain YM-1 was selected for further study based on its total phenolic compound (TP) content and antioxidant activity, which were enhanced by microbial fermentation. The extract fermented by YM-1 exhibited a superior TP content and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity compared to extracts fermented by other microbes. The highest TP content and DPPH radical scavenging activity were observed after one day of YM-1 fermentation. Yeast strain YM-1 was identified as Candida utilis based on an analysis of its physiological characteristics. During fermentation of the extract by C. utilis YM-1, no significant difference was observed in the proximate composition, including moisture, crude lipid, crude protein, and crude ash. Fermentation by C. utilis YM-1 resulted in enhanced biological activity, including increases in the TP content and antioxidant activity. Thus, fermentation by C. utilis YM-1 is an attractive strategy for developing value-added food ingredients.

[1]  강영미,et al.  유산균 발효에 의한 톳 (Hizikia fusiforme) 추출액의 항산화 및 항염증 활성 증가 , 2011 .

[2]  최종덕,et al.  Changes in Physicochemical and Sensory Properties of Hizikia fusiforme Water Extract by Fermentation of Lactic Acid Bacteria , 2011 .

[3]  J. Choi,et al.  Molecular docking studies of phlorotannins from Eisenia bicyclis with BACE1 inhibitory activity. , 2010, Bioorganic & medicinal chemistry letters.

[4]  S. Eom,et al.  Effect of Yeast Fermentation on the Antioxidant and Anti-inflammatory Activity of Sea Tangle Water Extract , 2010 .

[5]  Young Mog Kim,et al.  Improvement of the Functional Qualities of Sea Tangle Extract through Fermentation by Aspergillus oryzae , 2010 .

[6]  M. N. Qureshi,et al.  In-vitro Antioxidant and In-vivo Hepatoprotective activity of Leucas ciliata leaves , 2010 .

[7]  M. N. Qureshi,et al.  In-vitro antioxidant and in-vivo hepatoprotective activity of Leucas ciliata L. , 2010 .

[8]  Se-kwon Kim,et al.  α-Glucosidase and α-amylase inhibitory activities of phloroglucinal derivatives from edible marine brown alga, Ecklonia cava , 2009 .

[9]  M. Maegawa,et al.  Ecological studies of eisenia bicyclis (Kjellman) setchell and ecklonia cava Kjellman , 2007 .

[10]  K. Jeng,et al.  Effect of microbial fermentation on content of statin, GABA, and polyphenols in Pu-Erh tea. , 2007, Journal of agricultural and food chemistry.

[11]  K. Kang,et al.  Protective effect of Ecklonia cava enzymatic extracts on hydrogen peroxide-induced cell damage , 2006 .

[12]  Kil-Nam Kim,et al.  Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. , 2006, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[13]  S. Cui,et al.  Phenolic acid profiles and antioxidant activities of wheat bran extracts and the effect of hydrolysis conditions , 2006 .

[14]  D. Stewart,et al.  Different polyphenolic components of soft fruits inhibit α-amylase and α-glucosidase , 2005 .

[15]  Y. Jeon,et al.  Antioxidant activities of enzymatic extracts from brown seaweeds. , 2003, Bioresource technology.

[16]  C. Ruzafa,et al.  Influence of dietary arginine on the anabolic effects of androgens. , 2004, The Journal of endocrinology.

[17]  Y. Okada,et al.  A new phloroglucinol derivative from the brown alga Eisenia bicyclis: potential for the effective treatment of diabetic complications. , 2004, Journal of natural products.

[18]  Toshiyuki Shibata,et al.  Inhibitory effects of brown algal phlorotannins on secretory phospholipase A2s, lipoxygenases and cyclooxygenases , 2004, Journal of Applied Phycology.

[19]  M. Wisniewski,et al.  Characterization of extracellular lytic enzymes produced by the yeast biocontrol agent Candida oleophila , 2004, Current Genetics.

[20]  Toshiyuki Shibata,et al.  Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. , 2002, The Journal of antimicrobial chemotherapy.

[21]  Toshiyuki Shibata,et al.  Inhibitory activity of brown algal phlorotannins against hyaluronidase , 2002 .

[22]  S. Gündeş,et al.  Comparative performance of Fungichrom I, Candifast and API 20C Aux systems in the identification of clinically significant yeasts. , 2001, Journal of medical microbiology.

[23]  F. Saura-calixto,et al.  Antioxidant activity of fresh and processed edible seaweeds , 2001 .

[24]  O. Falade,et al.  The effect of processing on total organic acids content and mineral availability of simulated cassava-vegetable diets , 1999, Plant foods for human nutrition.

[25]  S. Achinewhu,et al.  Physicochemical properties and garification (gari yield) of selected cassava cultivars in Rivers State, Nigeria , 1998, Plant foods for human nutrition.

[26]  N. Misawa,et al.  Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts. , 1998, Journal of biotechnology.

[27]  K. Carroll,et al.  Comparison of updated Vitek Yeast Biochemical Card and API 20C yeast identification systems , 1994, Journal of clinical microbiology.

[28]  T. Sakata,et al.  Feeding modulation by pentose and hexose analogues. , 1992, The American journal of clinical nutrition.

[29]  S. Hashimoto,et al.  Studies on the antitumour activity of marine algae. , 1989 .

[30]  H. Verachtert,et al.  Purification and Characterization of Extracellular Amylolytic Enzymes from the Yeast Filobasidium capsuligenum , 1985, Applied and environmental microbiology.

[31]  M. Smogyi,et al.  Notes on sugar determination. , 1952, The Journal of biological chemistry.