Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules

Quorum sensing (QS) is a bacterial cell-to-cell communication system controlling QS-mediated genes which is synchronized with the population density. The regulation of specific gene activity is dependent on the signaling molecules produced, namely N-acyl homoserine lactones (AHLs). We report here the identification and characterization of AHLs produced by bacterial strain ND07 isolated from a Malaysian fresh water sample. Molecular identification showed that strain ND07 is clustered closely to Pseudomonas cremoricolorata. Spent culture supernatant extract of P. cremoricolorata strain ND07 activated the AHL biosensor Chromobacterium violaceum CV026. Using high resolution triple quadrupole liquid chromatography-mass spectrometry, it was confirmed that P. cremoricolorata strain ND07 produced N-octanoyl-l-homoserine lactone (C8-HSL) and N-decanoyl-l-homoserine lactone (C10-HSL). To the best of our knowledge, this is the first documentation on the production of C10-HSL in P. cremoricolorata strain ND07.

[1]  Diby Paul,et al.  Isolation and Molecular Characterization of Biofouling Bacteria and Profiling of Quorum Sensing Signal Molecules from Membrane Bioreactor Activated Sludge , 2014, International journal of molecular sciences.

[2]  Christopher M Thomas,et al.  Quorum-sensing-dependent regulation of biosynthesis of the polyketide antibiotic mupirocin in Pseudomonas fluorescens NCIMB 10586. , 2001, Microbiology.

[3]  Masataka Uchino,et al.  Recharacterization of Pseudomonas fulva Iizuka and Komagata 1963, and proposals of Pseudomonas parafulva sp. nov. and Pseudomonas cremoricolorata sp. nov. , 2001, The Journal of general and applied microbiology.

[4]  Frederick M. Ausubel,et al.  Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation , 2002, Nature.

[5]  Wai-Fong Yin,et al.  Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters , 2013, Sensors.

[6]  D. van den Broek,et al.  The Pseudomonas chlororaphis PCL1391 sigma regulator psrA represses the production of the antifungal metabolite phenazine-1-carboxamide. , 2005, Molecular plant-microbe interactions : MPMI.

[7]  Kok-Gan Chan,et al.  Malabaricone C from Myristica cinnamomea exhibits anti-quorum sensing activity. , 2011, Journal of natural products.

[8]  Kok-Gan Chan,et al.  Genome Sequence of Roseomonas sp. Strain B5, a Quorum-Quenching N-Acylhomoserine Lactone-Degrading Bacterium Isolated from Malaysian Tropical Soil , 2012, Journal of bacteriology.

[9]  B. Bassler.  How bacteria talk to each other: regulation of gene expression by quorum sensing. , 1999, Current opinion in microbiology.

[10]  V. Venturi.  Regulation of quorum sensing in Pseudomonas. , 2006, FEMS microbiology reviews.

[11]  Wai-Fong Yin,et al.  N-Acyl Homoserine Lactone Production by Klebsiella pneumoniae Isolated from Human Tongue Surface , 2012, Sensors.

[12]  M. Willcox,et al.  Determination of quorum-sensing signal molecules and virulence factors of Pseudomonas aeruginosa isolates from contact lens-induced microbial keratitis. , 2002, Journal of medical microbiology.

[13]  R. Haley,et al.  Dominant Role of Paraoxonases in Inactivation of the Pseudomonas aeruginosa Quorum-Sensing Signal N-(3-Oxododecanoyl)-l-Homoserine Lactone , 2008, Infection and Immunity.

[14]  K. M. Lee,et al.  Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[15]  U. Chaudhary,et al.  Quorum sensing and Bacterial Pathogenicity: From Molecules to Disease , 2011, Journal of laboratory physicians.

[16]  J. Ferkinghoff-Borg,et al.  Size of quorum sensing communities. , 2014, Molecular bioSystems.

[17]  Claudia Schmidt-Dannert,et al.  Applications of quorum sensing in biotechnology , 2010, Applied Microbiology and Biotechnology.

[18]  O. Vandeputte,et al.  Identification of Catechin as One of the Flavonoids from Combretum albiflorum Bark Extract That Reduces the Production of Quorum-Sensing-Controlled Virulence Factors in Pseudomonas aeruginosa PAO1 , 2009, Applied and Environmental Microbiology.

[19]  Li Ying Tan,et al.  Silencing Quorum Sensing through Extracts of Melicope lunu-ankenda , 2012, Sensors.

[20]  Wai-Fong Yin,et al.  Freshwater-Borne Bacteria Isolated from a Malaysian Rainforest Waterfall Exhibiting Quorum Sensing Properties , 2014, Sensors.

[21]  Wai-Fong Yin,et al.  Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract , 2012, Sensors.

[22]  J H Lamb,et al.  Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. , 1997, Microbiology.

[23]  Wai-Fong Yin,et al.  Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9 , 2013, Sensors.

[24]  L. Stetzenbach,et al.  Isolation, identification, and growth of well-water bacteria , 1986 .

[25]  P. Stewart,et al.  Daptomycin Rapidly Penetrates a Staphylococcus epidermidis Biofilm , 2009, Antimicrobial Agents and Chemotherapy.

[26]  K. Winzer,et al.  Look who's talking: communication and quorum sensing in the bacterial world , 2007, Philosophical Transactions of the Royal Society B: Biological Sciences.

[27]  L. Samaranayake,et al.  Microbial chemical signaling: a current perspective , 2012, Critical reviews in microbiology.

[28]  K. Winzer,et al.  Bacterial cell-to-cell communication: sorry, can't talk now - gone to lunch! , 2002, Current opinion in microbiology.

[29]  Wai-Fong Yin,et al.  Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves , 2013, Sensors.

[30]  M. Sebaihia,et al.  Carbapenem antibiotic production in Erwinia carotovora is regulated by CarR, a homologue of the LuxR transcriptional activator. , 1995, Microbiology.

[31]  A. Chatterjee,et al.  Genetic and physiological evidence for the production of N-acyl homoserine lactones by Pseudomonas syringae pv. syringae and other fluorescent plant pathogenic Pseudomonas species , 1998, European Journal of Plant Pathology.

[32]  Wai-Fong Yin,et al.  Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-l-homoserine Lactone , 2014, Sensors.