Effects of Adding Vindoline and MeJA on Production of Vincristine and Vinblastine, and Transcription of their Biosynthetic Genes in the Cultured CMCs of Catharanthus roseus

Vincristine and vinblastine were found by Liquid Chromatography-Mass Spectrometry (LC-MS) in Catharanthus roseus cambial meristem cells (CMCs) jointly treated with 0.25 mM vindolineand methyl jasmonate (MeJA), suggesting that C. roseus CMCs contain a complete set of the enzymes which are in response to convert vindoline into vincristine and vinblastine. Based on the facts that the transcript levels of vindoline-biosynthetic genes (STR, SGD and D4H) were up-regulated instead of being down-regulated by adding itself to the culture, and that the transcriptional factor ORCA3 was up-regulated simultaneously, we further confirmed that the transcription of STR, SGD, D4H was manipulated by ORCA3.

[1]  Jiachen Zi,et al.  Effects of β-cyclodextrin and methyl jasmonate on the production of vindoline, catharanthine, and ajmalicine in Catharanthus roseus cambial meristematic cell cultures , 2015, Applied Microbiology and Biotechnology.

[2]  Kexuan Tang,et al.  Overexpression of ORCA3 and G10H in Catharanthus roseus Plants Regulated Alkaloid Biosynthesis and Metabolism Revealed by NMR-Metabolomics , 2012, PloS one.

[3]  R. Deswal,et al.  New evidences about strictosidine synthase (Str) regulation by salinity, cold stress and nitric oxide in Catharanthus roseus , 2012, Journal of Plant Biochemistry and Biotechnology.

[4]  Yun Liu,et al.  Regulation of vinblastine biosynthesis in cell suspension cultures of catharanthus roseus , 2012, Plant Cell, Tissue and Organ Culture (PCTOC).

[5]  G. Loake,et al.  Cultured cambial meristematic cells as a source of plant natural products , 2010, Nature Biotechnology.

[6]  H. Liu,et al.  Overexpression of G10H and ORCA3 in the hairy roots of Catharanthus roseus improves catharanthine production , 2010, Plant Cell Reports.

[7]  K. San,et al.  Transcriptional response of the terpenoid indole alkaloid pathway to the overexpression of ORCA3 along with jasmonic acid elicitation of Catharanthus roseus hairy roots over time. , 2009, Metabolic engineering.

[8]  M. Jolicoeur,et al.  Analysis of Catharanthus roseus alkaloids by HPLC , 2007, Phytochemistry Reviews.

[9]  R. Verpoorte,et al.  Manipulating indole alkaloid production by Catharanthus roseus cell cultures in bioreactors: from biochemical processing to metabolic engineering , 2007, Phytochemistry Reviews.