Laser-engineered topography: correlation between structure dimensions and cell control

Topographical cues have a significant impact on cell responses and by this means, on the fabrication of innovative implant materials. However, analysis of cell-topography interactions in dependence of the surface feature dimensions is still challenging due to limitations in the fabrication technology. Here, we introduce surface structuring via picosecond laser systems, which enable a fast production of micro-sized topologies. Changes in the processing parameters further control the feature sizes of so-called spikes. Using surfaces with big and small spike-to-spike-distances for comparisons, we focussed on cell adhesion via extracellular matrix adsorption and focal adhesion complexes, morphology, localisation and proliferation of fibroblasts. The observed cell control was dependent on a turnover point related to the structure dimensions: only big spike-to-spike-distances reduced cell behaviour. Therefore, this technology offers a platform to study cell and tissue interactions with a defined microenvironment.

[1]  N. Kotov,et al.  Three-dimensional cell culture matrices: state of the art. , 2008, Tissue engineering. Part B, Reviews.

[2]  James M. Anderson,et al.  Biological Responses to Materials , 2001 .

[3]  Matthew J Dalby,et al.  The fibroblast response to tubes exhibiting internal nanotopography. , 2005, Biomaterials.

[4]  Julian H. George,et al.  Exploring and Engineering the Cell Surface Interface , 2005, Science.

[5]  Anaclet Ngezahayo,et al.  Cell cycle dependent regulation of gap junction coupling and apoptosis in GFSHR-17 granulosa cells , 2010 .

[6]  Jürgen Koch,et al.  Femtosecond Laser-Induced Surface Structures on Platinum and Their Effects on Surface Wettability and Fibroblast Cell Proliferation , 2009 .

[7]  Boris N. Chichkov,et al.  Laser-based nanoengineering of surface topographies for biomedical applications , 2011 .

[8]  S. Lo,et al.  Focal adhesions: what's new inside. , 2006, Developmental biology.

[9]  Benjamin M. Wu,et al.  Cell interaction with three-dimensional sharp-tip nanotopography. , 2007, Biomaterials.

[10]  Mathis O. Riehle,et al.  The use of materials patterned on a nano- and micro-metric scale in cellular engineering , 2002 .

[11]  Jürgen Koch,et al.  Femtosecond Laser Fabricated Spike Structures for Selective Control of Cellular Behavior , 2010, Journal of biomaterials applications.

[12]  R. Langer,et al.  Engineering substrate topography at the micro- and nanoscale to control cell function. , 2009, Angewandte Chemie.

[13]  C J Murphy,et al.  Effects of synthetic micro- and nano-structured surfaces on cell behavior. , 1999, Biomaterials.

[14]  T. Groth,et al.  The dependence of fibrillar adhesions in human fibroblasts on substratum chemistry. , 2006, Biomaterials.

[15]  U. Schwarz,et al.  Cell adhesion strength is controlled by intermolecular spacing of adhesion receptors. , 2010, Biophysical journal.

[16]  F. Goubard,et al.  Kinetics of conformational changes of fibronectin adsorbed onto model surfaces. , 2008, Colloids and surfaces. B, Biointerfaces.

[17]  Patricia J Keely,et al.  Focal adhesion regulation of cell behavior. , 2004, Biochimica et biophysica acta.

[18]  Molly M Stevens,et al.  Exploring and engineering the cell surface interface. , 2011, Science.

[19]  Boris N. Chichkov,et al.  Microreplication of laser-fabricated surface and three-dimensional structures , 2010 .

[20]  D. Ingber.  Tensegrity I. Cell structure and hierarchical systems biology , 2003, Journal of Cell Science.