Fabrication of Antibacterial Nanofibrous Membrane Infused with Essential Oil Extracted from Tea Tree for Packaging Applications

Nanofibers made by electrospinning are being applied to an unlimited number of applications. In this paper, we propose the fabrication of antimicrobial functional nanofibers infused with essential oil for packaging applications that can extend the shelf-life of fruits. The morphology of nanofibers with different concentrations of essential oil was characterized by SEM and mechanical enhancement was confirmed via universal testing machine (UTM). The surface chemistry and crystalline of the nanofibers were investigated by FTIR and XRD, respectively. The CO2 reduction study was carried out using a hand-made experimental apparatus and nanofiber hydrophobicity, which can prevent moisture penetration from the outside, was evaluated by contact angle. Antimicrobial properties of the functional nanofibers were estimated by using Gram-negative/positive bacteria. The cytotoxicity of the functional nanofibers was studied using fibroblast cells. Furthermore, this study investigated how long the shelf-life of tomatoes was extended. The nanofibers could serve as a multifunctional packaging, as an emerging technology in agricultural products, and even contribute to a better quality of various distributed agricultural products.

[1]  Shih-Jung Liu,et al.  Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers , 2016, International journal of nanomedicine.

[2]  P. Smolka,et al.  Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent , 2019, Polymers.

[3]  Ana Sanches-Silva,et al.  Use of essential oils in active food packaging: Recent advances and future trends , 2017 .

[4]  S. Kundu,et al.  Electrospinning: a fascinating fiber fabrication technique. , 2010, Biotechnology advances.

[5]  Artemis Stamboulis,et al.  Tailoring Crystallinity of Electrospun Plla Fibres by Control of Electrospinning Parameters , 2012 .

[6]  Bundit Jarimopas,et al.  Comparison of package cushioning materials to protect post‐harvest impact damage to apples , 2007 .

[7]  M. Kotaki,et al.  A review on polymer nanofibers by electrospinning and their applications in nanocomposites , 2003 .

[8]  Mira Park,et al.  TiO2 NPs Assembled into a Carbon Nanofiber Composite Electrode by a One-Step Electrospinning Process for Supercapacitor Applications , 2019, Polymers.

[9]  Sunil Kumar,et al.  Tea tree oil: a promising essential oil , 2017 .

[10]  T. Riley,et al.  Melaleuca alternifolia (Tea Tree) Oil: a Review of Antimicrobial and Other Medicinal Properties , 2006, Clinical Microbiology Reviews.

[11]  K. Cheung,et al.  Neural Repair , 2011 .

[12]  Jeum Kyu Hong,et al.  Application of Volatile Antifungal Plant Essential Oils for Controlling Pepper Fruit Anthracnose by Colletotrichum gloeosporioides , 2015, The plant pathology journal.

[13]  T. Riley,et al.  Safety, efficacy and provenance of tea tree (Melaleuca alternifolia) oil , 2001, Contact dermatitis.

[14]  Ludwig Erik Aguilar,et al.  Structural Packaging Technique Using Biocompatible Nanofiber with Essential Oil to Prolong the Shelf-Life of Fruit. , 2019, Journal of nanoscience and nanotechnology.

[15]  T. Satoh,et al.  Novel Multifunctional Luminescent Electrospun Fluorescent Nanofiber Chemosensor-Filters and Their Versatile Sensing of pH, Temperature, and Metal Ions , 2018, Polymers.

[16]  T. Peijs,et al.  High-modulus rotary jet spun co-polyimide nanofibers and their composites , 2020, Nanocomposites.

[17]  J. Mwasiagi,et al.  Electrospun cellulose acetate and poly(vinyl chloride) nanofiber mats containing silver nanoparticles for antifungi packaging , 2019, SN Applied Sciences.

[18]  T. Yamaoka,et al.  Surface Modification of Poly(L-lactic acid) Nanofiber with Oligo(D-lactic acid) Bioactive-Peptide Conjugates for Peripheral Nerve Regeneration , 2011 .

[19]  G. Rytwo,et al.  The Use of ATR-FTIR Spectroscopy for Quantification of Adsorbed Compounds , 2015 .

[20]  I. Southwell,et al.  Monoterpenoid accumulation in 1,8-cineole, terpinolene and terpinen-4-ol chemotypes of Melaleuca alternifolia seedlings. , 2003, Phytochemistry.

[21]  T. Riley,et al.  Effects of Melaleuca alternifolia (Tea Tree) Essential Oil and the Major Monoterpene Component Terpinen-4-ol on the Development of Single- and Multistep Antibiotic Resistance and Antimicrobial Susceptibility , 2011, Antimicrobial Agents and Chemotherapy.

[22]  U. Strotmann,et al.  Development and evaluation of a growth inhibition test with sewage bacteria for assessing bacterial toxicity of chemical compounds , 1994 .

[23]  M. Zalucki,et al.  Metabolism of 1,8-cineole in tea tree (Melaleuca alternifolia andM. linariifolia) by pyrgo beetle (Paropsisterna tigrina) , 1995, Journal of Chemical Ecology.

[24]  Lih-Geeng Chen,et al.  Correlations of the components of tea tree oil with its antibacterial effects and skin irritation , 2013 .

[25]  Joon-pyo Jeun,et al.  PREPARATION AND CHARACTERIZATION OF THE CARBON NANOFIBER MAT PRODUCED FROM ELECTROSPUN PAN/LIGNIN PRECURSORS BY ELECTRON BEAM IRRADIATION , 2011 .

[26]  Min Hee Kim,et al.  Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds , 2015, International journal of nanomedicine.

[27]  U. Strotmann,et al.  A growth inhibition test with sewage bacteria--results of an international ring test 1995. , 1996, Chemosphere.