Identification of PLCβ and PKC in pheromone receptor neurons of Antheraea polyphemus

Two proteins of the IP3 transduction pathway were identified by Western blots in homogenates of isolated pheromonesensitive sensilla of the silkmoth Antheraea polyphemus. A 110 kDa protein was recognized by an antiserum raised against the Drosophila phospholipase Cβ (PLCβ p121) and a 80 kDa protein was labelled by an antiserum against a synthetic peptide of a conserved region of protein kinase C (PKC). Incubation of homogenized sensory hairs with the main sex pheromone component, (E,Z) 6–11 hexadecadienyl acetate, resulted in a 6-fold increase in the activity of PKC compared to controls without pheromone. In contrast, incubation with pheromone did not affect the activity of protein kinase A (PKA). Activation of PKC by the membrane permeable dioctanoylglycerol led to excitation of the pheromone-sensitive receptor neurons. These data support the current concept that pheromone perception of moths is mediated by the IP3 transduction pathway.

[1]  R. Axel,et al.  A novel multigene family may encode odorant receptors: A molecular basis for odor recognition , 1991, Cell.

[2]  J Nikolić-Zugić,et al.  Role of self-peptides in positively selecting the T-cell repertoire. , 1990 .

[3]  A. Chess,et al.  Identification of candidate Drosophila olfactory receptors from genomic DNA sequence. , 1999, Genomics.

[4]  D. Raha,et al.  Requirement for a phospholipase C in odor response: overlap between olfaction and vision in Drosophila. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[5]  M. Laue,et al.  G-protein activation, identification and immunolocalization in pheromone-sensitive sensilla trichodea of moths , 1997, Cell and Tissue Research.

[6]  Monika Stengl,et al.  Perireceptor Events and Transduction Mechanisms in Insect Olfaction , 1999 .

[7]  Camilla Zack Strausfeld,et al.  Localized adaptation processes in olfactory sensilla of Saturniid moths , 1986 .

[8]  J. Brand,et al.  Experimental Cell Biology of Taste and Olfaction: Current Techniques and Protocols , 1995 .

[9]  T. Toyofuku,et al.  Mutation of glutamate 309 to glutamine alters one Ca(2+)-binding site in the Ca(2+)-ATPase of sarcoplasmic reticulum expressed in Sf9 cells. , 1993, The Journal of biological chemistry.

[10]  F. Zufall,et al.  Dual activation of a sex pheromone-dependent ion channel from insect olfactory dendrites by protein kinase C activators and cyclic GMP. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[11]  M. Stengl.  Intracellular-messenger-mediated cation channels in cultured olfactory receptor neurons. , 1993, The Journal of experimental biology.

[12]  J. Exton.  Regulation of phosphoinositide phospholipases by hormones, neurotransmitters, and other agonists linked to G proteins. , 1996, Annual review of pharmacology and toxicology.