Antimicrobial Activity of Peganum harmala and Heracleum persicum Against Acinetobacter baumannii

Background: The extracts of many plants have been used for their applications in the prevention of bacterial growth; however, these applications need more investigations. Objectives: The major aim of the current study was to investigate the antimicrobial activity of an extract of the Peganum harmala flower and Heracleum persicum against Acinetobacter baumannii. Materials and Methods: The minimum inhibitory (MIC) concentrations Minimum Bactericidal Concentration of extract and essential oil were investigated by microdilution method and antibiotic resistance was evaluated using the disk diffusion test. Results: In this study, the levels of MIC extract and essential oil of Peganum harmala were observed in ranges from 6.25 ppm to 12.5 ppm and 3.1 ppm to 25 ppm, respectively. The highest MIC value was observed as 12.5 ppm in A. baumannii. The levels of MIC extract and essential oil of Heracleum persicum were observed in ranges from 5 ppm to 20 ppm and 12.5 ppm to 10 ppm, respectively. The highest level of MIC extract and the highest essential oil value of Heracleum persicum were observed as 20 ppm and 10 ppm, respectively, in A. baumannii. Conclusions: Results of this study suggest that the extract alone of Peganum harmala and Heracleum persicum may be useful to treat bacterial infections.

[1]  H. Behzad,et al.  Antibacterial activity of the essential oils of endemic plants , 2012 .

[2]  H. Motamedi,et al.  Antibacterial activity of different parts of Peganum harmala L. growing in Iran against multi-drug resistant bacteria , 2011, EXCLI journal.

[3]  S. Faizi,et al.  Antibacterial activity in spices and local medicinal plants against clinical isolates of Karachi, Pakistan , 2011, Pharmaceutical biology.

[4]  A. Sowunmi,et al.  In vitro antiplasmodial activity and toxicity assessment of some plants from Nigerian ethnomedicine , 2011, Pharmaceutical biology.

[5]  Z. Mighri,et al.  Biological activities of Peganum harmala leaves , 2010 .

[6]  Ramin Sakhtianchi,et al.  Antitumor and antibacterial activity of four fractions from Heracleum persicum Desf. and Cinnamomum zeylanicum Blume , 2010 .

[7]  M. Aouni,et al.  Antibacterial, antiviral and antioxidant activities of aerial part extracts of Peganum harmala L. grown in Tunisia , 2010 .

[8]  H. Motamedi,et al.  Antibacterial activity of eight Iranian plant extracts against methicillin and cefixime restistant Staphylococcous aureus strains , 2010 .

[9]  A. G. Pirbaloutl.  Medicinal plants used in Chaharmahal and Bakhtyari districts of Iran. , 2009 .

[10]  Narender Singh,et al.  Efficient in vitro multiplication of Syrian Rue (Peganum harmala L.) using 6-benzylaminopurine pre- conditioned seedling explants , 2009 .

[11]  G. Rouhollah,et al.  Spectrophotometric Determination of Total Alkaloids in Peganum harmala L. Using Bromocresol Green , 2007 .

[12]  Mengming Michael Dong,et al.  Observations on the treatment of natural haemosporidia infections by total alkaloid ofPeganum harmala L. in cattle , 1997, Tropical Animal Health and Production.

[13]  Guangling Li,et al.  Effect of total alkaloid ofPeganum harmala L. in the treatment of experimental haemosporidian infections in cattle , 2007, Tropical Animal Health and Production.

[14]  P. Sansonetti,et al.  The Genus Shigella , 2006 .

[15]  P. Salehian,et al.  Toxicity of Peganum harmala: Review and a Case Report , 2002 .

[16]  C. F. Chen,et al.  Vasorelaxant effect of harman. , 2000, European journal of pharmacology.

[17]  J. González-Maeso,et al.  Characterization of receptor-mediated [35S]GTPgammaS binding to cortical membranes from postmortem human brain. , 2000, European journal of pharmacology.

[18]  M. M. Cowan.  Plant Products as Antimicrobial Agents , 1999, Clinical Microbiology Reviews.

[19]  D. Prashanth,et al.  Antibacterial activity of Peganum harmala , 1999 .

[20]  R. Ali,et al.  The aging paradox: free radical theory of aging , 1999, Experimental Gerontology.

[21]  L. McDonald,et al.  Seasonal variation of Acinetobacter infections: 1987-1996. Nosocomial Infections Surveillance System. , 1999, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[22]  Z. Ling,et al.  Effect of total alkaloid of Peganum harmala L. in the treatment of experimental haemosporidian infections in cattle. , 1997 .

[23]  K. Mitchelson,et al.  Improved separation of six harmane alkaloids by high-performance capillary electrophoresis , 1997 .

[24]  S. Matlin,et al.  Four flavonoid glycosides from Peganum harmala. , 1997, Phytochemistry.

[25]  M. Hadidi,et al.  Direct Relaxant Effect of Peganum Harmala Seed Extract on Smooth Muscles of Rabbit and Guinea Pig , 1991 .

[26]  R. Hiltunen,et al.  Composition of Essential Oil of Heracleum persicum Fruits. , 1984, Planta medica.

[27]  Philip Smith.  Seasonal incidence of Acinetobacter infection. , 1979, The Journal of infectious diseases.

[28]  G. J. Adams.  Intracerebral infection of mice with high-virulence and low-virulence strains of Bordetella pertussis. , 1970, Journal of medical microbiology.