Edge detection in images using Marr-Hildreth filtering techniques

Details of the morphology of light microscope images of horseradish peroxidase labeled mammalian neurons in cell culture were investigated. A modified Marr-Hildreth edge-detecting algorithm was used in an image processor to obtain a continuous border of the labeled neurons. The interior of the border was filled to obtain isolated binary silhouettes of the neurons. These silhouettes can be used for further quantitative studies.