FLUORIDE INHIBITION OF OXYGEN CONSUMPTION AND INCREASED OXIDATIVE STRESS IN RATS

The aim of this work was to evaluate the effect of fluoride (F) on oxygen consumption (VO2) in rats and how it might affect the respiratory chain and the levels of reactive oxygen species (ROS). Eighteen Sprague-Dawley rats were divided into three groups: Control, NaF20, and NaF40, which received 0, 20, and 40 μmol F/100 g bw/day for 30 days, respectively. In vivo, VO2 decreased 90 min after treatment with F, whereas TBARS, CAT, and GPx were higher. In vitro, F decreased VO2 in liver and in mitochondria. These results support the hypothesis that the bone inflammatory foci observed in treatments with NaF could involve an inhibition of the respiratory chain that would cause increase in reactive oxygen species (ROS).

[1]  H. Cui,et al.  Decreased antioxidase activities and oxidative stress in the spleen of chickens fed on high-fluorine diets , 2011, Human & experimental toxicology.

[2]  C. Farquharson,et al.  Sodium fluoride induces apoptosis and alters bcl-2 family protein expression in MC3T3-E1 osteoblastic cells. , 2011, Biochemical and biophysical research communications.

[3]  I. Baranowska-Bosiacka,et al.  Fluoride as a pro-inflammatory factor and inhibitor of ATP bioavailability in differentiated human THP1 monocytic cells. , 2010, Toxicology letters.

[4]  A. Rigalli,et al.  Experimental Surgical Models in the Laboratory Rat , 2009 .

[5]  E. B. Tahara,et al.  Tissue-, substrate-, and site-specific characteristics of mitochondrial reactive oxygen species generation. , 2009, Free radical biology & medicine.

[6]  Enrique Cadenas,et al.  On the mechanism and biology of cytochrome oxidase inhibition by nitric oxide. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[7]  E. Cadenas,et al.  Regulation of mitochondrial respiration by adenosine diphosphate, oxygen, and nitric oxide. , 1999, Methods in enzymology.

[8]  A. Rigalli,et al.  Measurement of total and diffusible serum fluoride , 1999, Journal of clinical laboratory analysis.

[9]  J. Poderoso,et al.  Liver oxygen uptake dependence and mitochondrial function in septic rats. , 1994, Circulatory shock.

[10]  A. Rigalli,et al.  Pharmacokinetic differences between sodium fluoride and sodium monofluorophosphate and comparative bone mass increasing activity of both compounds in the rat. , 1994, Arzneimittel-Forschung.

[11]  E. Olfert,et al.  Guide to the care and use of experimental animals , 1993 .

[12]  大川 博,et al.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction , 1979 .

[13]  D. Bass,et al.  Polymorphonuclear Leukocyte Bactericidal Activity and Oxidative Metabolism During Glutathione Peroxidase Deficiency , 1977, Infection and immunity.