Pulmonary responses to printer toner particles in mice after intratracheal instillation.

The release of ultrafine particles from office equipment is currently receiving great concerns due to its potential threat to human health when inhaled. Printer toner is one of the largest consumables in daily office work, and the particles released from printers and photocopiers may pose damage to respiratory system. In this study, we found the particles can be released into the surrounding environment during the printing process and the concentrations of PM(2.5) and PM(10) particles increased obviously. To evaluate the time-course pulmonary responses caused by toner particles, the toner suspension was instilled into the lungs of the male mice through intratracheally instillation every other day for four times and the pulmonary responses of the lung were monitored at days 9, 28, 56 and 84. Indeed, mice treated with toner particles displayed a slower body weight growth rate during the recovery phase. The total cell number in bronchoalveolar lavage fluids (BALF) of toner-exposed groups was much higher than the saline-treated groups. The total protein, lactate dehydrogenase and acid phosphatase in BALF exhibited significant changes (p<0.05 or p<0.01) at different time points. The nitric oxide synthase, interleukin 1-beta, and interleukin 6 in the lung tissue of the toner-exposed groups also exhibited significant changes (p<0.05 or p<0.01). The pathological examination showed that toner particles can adhere to the alveolar septal walls, then enter into the alveoli and cause pulmonary lesion. During the experimental period, particles phagocytosed by alveolar macrophages (AMs) led to an increase of both AMs number and apoptosis. The pulmonary stress still remained over time even with a clearance period for 12 weeks. These results indicate that exposure to toner particles can inhibit the normal growth of the mice and induce significant inflammatory responses and lesion in the lung tissues. The health and safety effects from working indoors in offices with fumes and particles released from photocopiers and printers need to be paid more attention.

[1]  B. Corrin,et al.  Ultrastructural localization of acid phosphatase in the rat lung. , 1969, Journal of anatomy.

[2]  K. Honda,et al.  Examination of mRNA expression in rat hearts and lungs for analysis of effects of exposure to concentrated ambient particles on cardiovascular function. , 2008, Toxicology.

[3]  Der-Jen Hsu,et al.  Measurements of fine and ultrafine particles formation in photocopy centers in Taiwan , 2007 .

[4]  Mark J. Mendell,et al.  Phase 1 of the California Healthy Building Study: A Summary , 1993 .

[5]  J. West,et al.  The Differential Cytotoxicity of Water-Soluble Fullerenes , 2004 .

[6]  J. Byeon,et al.  Emission of submicron aerosol particles in operating a laser beam printer , 2009 .

[7]  J E Camp,et al.  Upper respiratory irritation from controlled exposure to vapor from carbonless copy forms. , 1986, Journal of occupational medicine. : official publication of the Industrial Medical Association.

[8]  Yongjun Li,et al.  Comparative study on the acute pulmonary toxicity induced by 3 and 20nm TiO(2) primary particles in mice. , 2007, Environmental toxicology and pharmacology.

[9]  P. Morrow,et al.  LUNG CLEARANCE AND RETENTION OF TONER, TiO2, AND CRYSTALLINE SILICA, UTILIZING A TRACER TECHNIQUE DURING CHRONIC INHALATION EXPOSURE IN SYRIAN GOLDEN HAMSTERS , 1998 .

[10]  L. Ignarro,et al.  Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. , 1993, Biochemical and biophysical research communications.

[11]  B. Ostro,et al.  Air pollution and emergency room visits for asthma in Santa Clara County, California. , 1997, Environmental health perspectives.

[12]  Z. Chai,et al.  Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration. , 2007, Toxicology letters.

[13]  B. V. Pedersen,et al.  Influence of personal characteristics, job-related factors and psychosocial factors on the sick building syndrome. Danish Indoor Climate Study Group. , 1989, Scandinavian journal of work, environment & health.

[14]  H. K. Fai,et al.  Characterization of VOCs, ozone, and PM10 emissions from office equipment in an environmental chamber , 2001 .

[15]  Alessandra Spinali,et al.  Effects of particulate matter (PM(10), PM(2.5) and PM(1)) on the cardiovascular system. , 2009, Toxicology.

[16]  Meng Wang,et al.  Comparative study of pulmonary responses to nano- and submicron-sized ferric oxide in rats. , 2008, Toxicology.

[17]  S. Weichenthal,et al.  Indoor ultrafine particles and childhood asthma: exploring a potential public health concern. , 2007, Indoor air.

[18]  J. Schwartz,et al.  PM10, ozone, and hospital admissions for the elderly in Minneapolis-St. Paul, Minnesota. , 1994, Archives of environmental health.

[19]  P. Morrow,et al.  Pulmonary response to toner upon chronic inhalation exposure in rats. , 1991, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[20]  M. Salter,et al.  Differential induction of brain, lung and liver nitric oxide synthase by endotoxin in the rat. , 1990, The Biochemical journal.

[21]  V. Castranova,et al.  Oxidative stress and inflammatory response in dermal toxicity of single-walled carbon nanotubes. , 2009, Toxicology.

[22]  Lidia Morawska,et al.  Particle emission characteristics of office printers. , 2007, Environmental science & technology.

[23]  V. Castranova,et al.  Intratracheal instillation of silica up-regulates inducible nitric oxide synthase gene expression and increases nitric oxide production in alveolar macrophages and neutrophils. , 1994, American journal of respiratory cell and molecular biology.

[24]  Wei Li,et al.  Time-dependent translocation and potential impairment on central nervous system by intranasally instilled TiO(2) nanoparticles. , 2008, Toxicology.

[25]  S. Vesper,et al.  Dose-dependent allergic responses to an extract of Penicillium chrysogenum in BALB/c mice. , 2005, Toxicology.

[26]  P. Wolkoff,et al.  Photocopiers and indoor air pollution , 1999 .

[27]  M. Trotta,et al.  An overview on the toxicity of inhaled nanoparticles , 2006 .

[28]  B. De Berardis,et al.  Inflammatory mediators induced by coarse (PM2.5-10) and fine (PM2.5) urban air particles in RAW 264.7 cells. , 2003, Toxicology.

[29]  D. Dockery,et al.  An association between air pollution and mortality in six U.S. cities. , 1993, The New England journal of medicine.

[30]  H. Karlsson,et al.  Size-dependent toxicity of metal oxide particles--a comparison between nano- and micrometer size. , 2009, Toxicology letters.

[31]  M. Beck,et al.  Allergic contact dermatitis to crystal violet in carbonless copy paper , 1987 .

[32]  P. Baron,et al.  Exposure to Carbon Nanotube Material: Assessment of Nanotube Cytotoxicity using Human Keratinocyte Cells , 2003, Journal of toxicology and environmental health. Part A.

[33]  R Weissleder,et al.  Superparamagnetic iron oxide: pharmacokinetics and toxicity. , 1989, AJR. American journal of roentgenology.

[34]  B. Rehn,et al.  Ein neues In-vitro-Prüfkonzept (Vektorenmodell) zum biologischen Screening und Monitoring der Lungentoxizität von Stäuben : Darstellung des Konzeptes und Überprüfung mit Stöuben bekannter Wirkung , 1999 .

[35]  R. Mermelstein,et al.  Acute Toxicity Studies of Xerox Reprographic Toners , 1994 .

[36]  Michael Wensing,et al.  An investigation into the characteristics and formation mechanisms of particles originating from the operation of laser printers. , 2009, Environmental science & technology.

[37]  A. Woolcock,et al.  A review of the racial differences in the lung function of normal Caucasian, Chinese and Indian subjects. , 1991, The European respiratory journal.

[38]  Wei Li,et al.  Potential neurological lesion after nasal instillation of TiO(2) nanoparticles in the anatase and rutile crystal phases. , 2008, Toxicology letters.

[39]  J. Gearhart,et al.  In vitro toxicity of nanoparticles in BRL 3A rat liver cells. , 2005, Toxicology in vitro : an international journal published in association with BIBRA.

[40]  W. J. Brock,et al.  Comparative pulmonary toxicity inhalation and instillation studies with different TiO2 particle formulations: impact of surface treatments on particle toxicity. , 2005, Toxicological sciences : an official journal of the Society of Toxicology.

[41]  C. Northeim,et al.  Office Equipment: Design, Indoor Air Emissions, and Pollution Prevention Opportunities , 1995 .

[42]  J. Nath,et al.  Pro-inflammatory responses of human bronchial epithelial cells to acute nitrogen dioxide exposure. , 2004, Toxicology.

[43]  W. Koch,et al.  PULMONARY RESPONSE TO TONER, TIO2 AND CRYSTALLINE SILICA UPON CHRONIC INHALATION EXPOSURE IN SYRIAN GOLDEN HAMSTERS , 1998 .

[44]  Patrizia Santi,et al.  Toxicity of antimony trioxide nanoparticles on human hematopoietic progenitor cells and comparison to cell lines. , 2009, Toxicology.

[45]  Michael Wensing,et al.  Evaluation of ultrafine particle emissions from laser printers using emission test chambers. , 2008, Environmental science & technology.

[46]  H. Ishida,et al.  Nitrotyrosine formation and its role in various pathological conditions , 2000, Free radical research.

[47]  T. Estlander,et al.  Occupational allergic contact dermatitis caused by diethylenetriamine in carbonless copy paper , 1993, Contact dermatitis.

[48]  A. Peters,et al.  Short-term effects of particulate air pollution on respiratory morbidity in asthmatic children. , 1997, The European respiratory journal.

[49]  N. Cacalano,et al.  Potential contribution of naïve immune effectors to oral tumor resistance: role in synergistic induction of VEGF, IL-6, and IL-8 secretion , 2008, Cancer Immunology, Immunotherapy.

[50]  M. Jaakkola,et al.  Office equipment and supplies: a modern occupational health concern? , 1999, American journal of epidemiology.

[51]  W. Koch,et al.  Subchronic Inhalation Study of Toner in Rats , 1990 .

[52]  Michael Wensing,et al.  Particle Measurement of Hardcopy Devices , 2006 .

[53]  Kostas Kostarelos,et al.  The long and short of carbon nanotube toxicity , 2008, Nature Biotechnology.

[54]  H. Vapaatalo,et al.  Nitric oxide in inflammation and immune response. , 1995, Annals of medicine.

[55]  F. J. Miller,et al.  Correlation of effects of inhaled versus intratracheally injected males on susceptibility to respiratory infection in mice. , 1981, The American review of respiratory disease.

[56]  Michael Wensing,et al.  Characterization of Ultra-fine Particle Emissions from a Laser Printer , 2006 .

[57]  P. Baron,et al.  Inhalation vs. aspiration of single-walled carbon nanotubes in C57BL/6 mice: inflammation, fibrosis, oxidative stress, and mutagenesis. , 2008, American journal of physiology. Lung cellular and molecular physiology.

[58]  T. Xia,et al.  Toxic Potential of Materials at the Nanolevel , 2006, Science.

[59]  Shuji Fujii,et al.  Indoor air quality for chemical and ultrafine particle contaminants from printers , 2007 .

[60]  J. Stamler,et al.  The biology of nitrogen oxides in the airways. , 1994, American journal of respiratory and critical care medicine.

[61]  J. Everitt,et al.  Pulmonary responses of mice, rats, and hamsters to subchronic inhalation of ultrafine titanium dioxide particles. , 2004, Toxicological sciences : an official journal of the Society of Toxicology.

[62]  A. Hodgson,et al.  Indoor pollutants emitted by office equipment: A review of reported data and information needs , 2008 .

[63]  Jan Sundell,et al.  A Prevalence Study Of The Sick Building Syndrome (SBS) And Facial Skin Symptoms In Office Workers , 1993 .

[64]  T. Casale,et al.  Acute systemic reactions to carbonless copy paper associated with histamine release. , 1988, JAMA.

[65]  J. Martens,et al.  Oxidative stress and proinflammatory effects of carbon black and titanium dioxide nanoparticles: role of particle surface area and internalized amount. , 2009, Toxicology.

[66]  Meng Wang,et al.  Particokinetics and extrapulmonary translocation of intratracheally instilled ferric oxide nanoparticles in rats and the potential health risk assessment. , 2009, Toxicological sciences : an official journal of the Society of Toxicology.

[67]  J. Nagy,et al.  Respiratory toxicity of multi-wall carbon nanotubes. , 2005, Toxicology and applied pharmacology.

[68]  J. Pollock,et al.  Genomic analysis and expression patterns reveal distinct genes for endothelial and brain nitric oxide synthase. , 1993, Hypertension.

[69]  B. Arborgh,et al.  Fine Structural Localization of Acid Phosphatase , 1977 .

[70]  T. Webb,et al.  Pulmonary toxicity study in rats with three forms of ultrafine-TiO2 particles: differential responses related to surface properties. , 2007, Toxicology.

[71]  J. Samet,et al.  Air Pollution and Cardiovascular Disease: A Statement for Healthcare Professionals From the Expert Panel on Population and Prevention Science of the American Heart Association , 2004, Circulation.

[72]  M. Hsiao,et al.  Biocompatibility of Fe3O4 nanoparticles evaluated by in vitro cytotoxicity assays using normal, glia and breast cancer cells , 2010, Nanotechnology.

[73]  F. Seiler,et al.  Investigations on the inflammatory and genotoxic lung effects of two types of titanium dioxide: untreated and surface treated. , 2003, Toxicology and applied pharmacology.

[74]  X. Basagaña,et al.  Particles, and not gases, are associated with the risk of death in patients with chronic obstructive pulmonary disease. , 2001, International journal of epidemiology.

[75]  F. Dominici,et al.  Fine particulate air pollution and mortality in 20 U.S. cities, 1987-1994. , 2000, The New England journal of medicine.