Combinatorial biosynthesis of reduced polyketides

[1]  C. Townsend,et al.  New images evoke FAScinating questions. , 2006, Chemistry & biology.

[2]  John R Carney,et al.  Combinatorial polyketide biosynthesis by de novo design and rearrangement of modular polyketide synthase genes , 2005, Nature Biotechnology.

[3]  F. Arnold,et al.  Evolving strategies for enzyme engineering. , 2005, Current opinion in structural biology.

[4]  J. Errey,et al.  Probing the breadth of macrolide glycosyltransferases: in vitro remodeling of a polyketide antibiotic creates active bacterial uptake and enhances potency. , 2005, Journal of the American Chemical Society.

[5]  Hugo Gramajo,et al.  Production of the Potent Antibacterial Polyketide Erythromycin C in Escherichia coli , 2005, Applied and Environmental Microbiology.

[6]  Rolf Müller,et al.  Heterologous expression of a myxobacterial natural products assembly line in pseudomonads via red/ET recombineering. , 2005, Chemistry & biology.

[7]  Gideon Schreiber,et al.  Folding and binding: an extended family business , 2005 .

[8]  Mark Welch,et al.  Genetic approaches to polyketide antibiotics. 1. , 2005, Chemical reviews.

[9]  P. Bartel,et al.  Expression of polyketide biosynthesis and regulatory genes in heterologous streptomycetes , 1991, Journal of Industrial Microbiology.

[10]  Ó. Salazar,et al.  A novel actinomycete strain de-replication approach based on the diversity of polyketide synthase and nonribosomal peptide synthetase biosynthetic pathways , 2005, Applied Microbiology and Biotechnology.

[11]  Kira J Weissman,et al.  Polyketide biosynthesis: understanding and exploiting modularity , 2004, Philosophical Transactions of the Royal Society of London. Series A: Mathematical, Physical and Engineering Sciences.

[12]  The role of erythromycin C-12 hydroxylase, EryK, as a substitute for PikC hydroxylase in pikromycin biosynthesis. , 2004, Bioorganic chemistry.

[13]  Neil Bate,et al.  Positive control of tylosin biosynthesis: pivotal role of TylR , 2004, Molecular microbiology.

[14]  Kedar Patel,et al.  Engineered biosynthesis of geldanamycin analogs for Hsp90 inhibition. , 2004, Chemistry & biology.

[15]  Matthias Platzer,et al.  Antitumor polyketide biosynthesis by an uncultivated bacterial symbiont of the marine sponge Theonella swinhoei. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[16]  Chu-Young Kim,et al.  Reconstituting modular activity from separated domains of 6-deoxyerythronolide B synthase. , 2004, Biochemistry.

[17]  Sarah J Kodumal,et al.  Total synthesis of long DNA sequences: synthesis of a contiguous 32-kb polyketide synthase gene cluster. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[18]  L. Katz,et al.  Production of hybrid 16-membered macrolides by expressing combinations of polyketide synthase genes in engineered Streptomyces fradiae hosts. , 2004, Chemistry & biology.

[19]  Pascal Simonet,et al.  Phylogenetic Analysis of Polyketide Synthase I Domains from Soil Metagenomic Libraries Allows Selection of Promising Clones , 2004, Applied and Environmental Microbiology.

[20]  Young-Soo Hong,et al.  Inactivation of the carbamoyltransferase gene refines post-polyketide synthase modification steps in the biosynthesis of the antitumor agent geldanamycin. , 2004, Journal of the American Chemical Society.

[21]  Leonard Katz,et al.  Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae , 2004, Applied Microbiology and Biotechnology.

[22]  William H Gerwick,et al.  Structure and biosynthesis of the jamaicamides, new mixed polyketide-peptide neurotoxins from the marine cyanobacterium Lyngbya majuscula. , 2004, Chemistry & biology.

[23]  Christopher T Walsh,et al.  Polyketide and Nonribosomal Peptide Antibiotics: Modularity and Versatility , 2004, Science.

[24]  David H Sherman,et al.  An efficient method for creation and functional analysis of libraries of hybrid type I polyketide synthases. , 2004, Protein engineering, design & selection : PEDS.

[25]  D. Sherman,et al.  Merging the potential of microbial genetics with biological and chemical diversity: an even brighter future for marine natural product drug discovery. , 2004, Natural product reports.

[26]  Sarojini Adusumilli,et al.  Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulcerans. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[27]  Hui Hong,et al.  Identification of a Phosphopantetheinyl Transferase for Erythromycin Biosynthesis in Saccharopolyspora erythraea , 2004, Chembiochem : a European journal of chemical biology.

[28]  A. Minami,et al.  Cloning, Sequencing, and Functional Analysis of the Biosynthetic Gene Cluster of Macrolactam Antibiotic Vicenistatin in , 2004 .

[29]  Yasushi Ogasawara,et al.  Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. , 2004, Chemistry & biology.

[30]  J. Galazzo,et al.  Improved Bioconversion of 15‐Fluoro‐6‐deoxyerythronolide B to 15‐Fluoro‐erythromycin A by Overexpression of the eryK Gene in Saccharopolyspora erythraea , 2004, Biotechnology progress.

[31]  R. Müller Don't classify polyketide synthases. , 2004, Chemistry & biology.

[32]  K. Chater,et al.  Lambda red-mediated genetic manipulation of antibiotic-producing Streptomyces. , 2004, Advances in applied microbiology.

[33]  O. Genilloud,et al.  New PCR Primers for the Screening of NRPS and PKS-I Systems in Actinomycetes: Detection and Distribution of These Biosynthetic Gene Sequences in Major Taxonomic Groups , 2004, Microbial Ecology.

[34]  R. Müller,et al.  Myxobacteria: proficient producers of novel natural products with various biological activities--past and future biotechnological aspects with the focus on the genus Sorangium. , 2003, Journal of biotechnology.

[35]  T. Weber,et al.  Exploiting the genetic potential of polyketide producing streptomycetes. , 2003, Journal of biotechnology.

[36]  J. Kealey,et al.  6-Deoxyerythronolide B analogue production in Escherichia coli through metabolic pathway engineering. , 2003, Biochemistry.

[37]  C. Hertweck,et al.  Iteration as programmed event during polyketide assembly; molecular analysis of the aureothin biosynthesis gene cluster. , 2003, Chemistry & biology.

[38]  Gregory L. Challis,et al.  Synergy and contingency as driving forces for the evolution of multiple secondary metabolite production by Streptomyces species , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Christine J. Martin,et al.  Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthase. , 2003, Organic & biomolecular chemistry.

[40]  L. Bai,et al.  The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. , 2003, Journal of the American Chemical Society.

[41]  Christopher T. Walsh,et al.  Biosynthesis of Yersiniabactin, a Complex Polyketide-Nonribosomal Peptide, Using Escherichia coli as a Heterologous Host , 2003, Applied and Environmental Microbiology.

[42]  D. Cane,et al.  Enhancing the modularity of the modular polyketide synthases: transacylation in modular polyketide synthases catalyzed by malonyl-CoA:ACP transacylase. , 2003, Journal of the American Chemical Society.

[43]  Kenji Watanabe,et al.  Understanding Substrate Specificity of Polyketide Synthase Modules by Generating Hybrid Multimodular Synthases* , 2003, Journal of Biological Chemistry.

[44]  K. Reynolds,et al.  Substrate recognition and channeling of monomodules from the pikromycin polyketide synthase. , 2003, Journal of the American Chemical Society.

[45]  C. Walsh Opinion — anti-infectives: Where will new antibiotics come from? , 2003, Nature Reviews Microbiology.

[46]  C. Starks,et al.  Isolation and characterization of new epothilone analogues from recombinant Myxococcus xanthus fermentations. , 2003, Journal of natural products.

[47]  James Staunton,et al.  Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization , 2003, Molecular microbiology.

[48]  S. Donadio,et al.  Strategies for combinatorial biosynthesis with modular polyketide synthases. , 2003, Combinatorial chemistry & high throughput screening.

[49]  Sabine Gaisser,et al.  Direct production of ivermectin-like drugs after domain exchange in the avermectin polyketide synthase of Streptomyces avermitilis ATCC31272. , 2003, Organic & biomolecular chemistry.

[50]  Kira J Weissman,et al.  The structure of docking domains in modular polyketide synthases. , 2003, Chemistry & biology.

[51]  Kenji Watanabe,et al.  Engineered biosynthesis of an ansamycin polyketide precursor in Escherichia coli , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[52]  R. Firn,et al.  Natural products--a simple model to explain chemical diversity. , 2003, Natural product reports.

[53]  Patrick Caffrey,et al.  Conserved Amino Acid Residues Correlating With Ketoreductase Stereospecificity in Modular Polyketide Synthases , 2003, Chembiochem : a European journal of chemical biology.

[54]  Andrzej Witkowski,et al.  Structural and functional organization of the animal fatty acid synthase. , 2003, Progress in lipid research.

[55]  James Staunton,et al.  A novel erythromycin, 6-desmethyl erythromycin D, made by substituting an acyltransferase domain of the erythromycin polyketide synthase. , 2003, The Journal of antibiotics.

[56]  J. Staunton,et al.  Active-site residue, domain and module swaps in modular polyketide synthases , 2003, Journal of Industrial Microbiology and Biotechnology.

[57]  C. Walsh,et al.  Chemoenzymatic route to macrocyclic hybrid peptide/polyketide-like molecules. , 2003, Journal of the American Chemical Society.

[58]  Christopher M Thomas,et al.  Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586. , 2003, Chemistry & biology.

[59]  C. Hutchinson,et al.  Rapid engineering of polyketide overproduction by gene transfer to industrially optimized strains , 2003, Journal of Industrial Microbiology and Biotechnology.

[60]  Ben Shen,et al.  Polyketide biosynthesis beyond the type I, II and III polyketide synthase paradigms. , 2003, Current opinion in chemical biology.

[61]  P. Leadlay,et al.  Mammalian fatty acid synthase: closure on a textbook mechanism? , 2003, Chemistry & biology.

[62]  Rahul M Kohli,et al.  Enzymology of acyl chain macrocyclization in natural product biosynthesis. , 2003, Chemical communications.

[63]  Brian O. Bachmann,et al.  A genomics-guided approach for discovering and expressing cryptic metabolic pathways , 2003, Nature Biotechnology.

[64]  C Richard Hutchinson,et al.  A model of structure and catalysis for ketoreductase domains in modular polyketide synthases. , 2003, Biochemistry.

[65]  Pascale Jeannin,et al.  Recombinant Environmental Libraries Provide Access to Microbial Diversity for Drug Discovery from Natural Products , 2003, Applied and Environmental Microbiology.

[66]  Andreas Schirmer,et al.  Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. , 2003, FEMS microbiology letters.

[67]  Jose V. Lopez,et al.  Polyketide Synthase Genes from Marine Dinoflagellates , 2003, Marine Biotechnology.

[68]  J. Gil,et al.  Polyene antibiotic biosynthesis gene clusters , 2003, Applied Microbiology and Biotechnology.

[69]  Bernard R Brooks,et al.  Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machine , 2002, The EMBO journal.

[70]  Jörn Piel,et al.  A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[71]  R. Stroud,et al.  Insights into channel architecture and substrate specificity from crystal structures of two macrocycle-forming thioesterases of modular polyketide synthases. , 2002, Biochemistry.

[72]  U. Rix,et al.  Modification of post-PKS tailoring steps through combinatorial biosynthesis. , 2002, Natural product reports.

[73]  Wen Liu,et al.  Biosynthesis of the Enediyne Antitumor Antibiotic C-1027 , 2002, Science.

[74]  J. Thorson,et al.  The Calicheamicin Gene Cluster and Its Iterative Type I Enediyne PKS , 2002, Science.

[75]  Zhihao Hu,et al.  Process and Metabolic Strategies for Improved Production of Escherichia coli-Derived 6-Deoxyerythronolide B , 2002, Applied and Environmental Microbiology.

[76]  Z. Deng,et al.  [Construction of a temperature inducible shuttle expression vector and its application in Streptomyces]. , 2002, Sheng wu gong cheng xue bao = Chinese journal of biotechnology.

[77]  N. Bate,et al.  Genetic engineering of aminodeoxyhexose biosynthesis in Streptomyces fradiae , 2002, Nature Biotechnology.

[78]  Christine J. Martin,et al.  Increasing the efficiency of heterologous promoters in actinomycetes. , 2002, Journal of molecular microbiology and biotechnology.

[79]  L. Bai,et al.  The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[80]  B. Barrell,et al.  Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) , 2002, Nature.

[81]  R. Lill,et al.  Parallel pathways for oxidation of 14‐membered polyketide macrolactones in Saccharopolyspora erythraea , 2002, Molecular microbiology.

[82]  James Staunton,et al.  Engineering specificity of starter unit selection by the erythromycin‐producing polyketide synthase , 2002, Molecular microbiology.

[83]  K. Reynolds,et al.  Generation of multiple bioactive macrolides by hybrid modular polyketide synthases in Streptomyces venezuelae. , 2002, Chemistry & biology.

[84]  D. Cane,et al.  Precursor-directed biosynthesis: biochemical basis of the remarkable selectivity of the erythromycin polyketide synthase toward unsaturated triketides. , 2002, Chemistry & biology.

[85]  L. Miercke,et al.  Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: Versatility from a unique substrate channel , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[86]  B. Lugtenberg Faculty Opinions recommendation of Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. , 2001 .

[87]  C R Hutchinson,et al.  Alteration of the substrate specificity of a modular polyketide synthase acyltransferase domain through site-specific mutations. , 2001, Biochemistry.

[88]  Keith A. Powell,et al.  Directed Evolution and Biocatalysis. , 2001, Angewandte Chemie.

[89]  D. Sherman,et al.  Isolation and structure determination of novamethymycin, a new bioactive metabolite of the methymycin biosynthetic pathway in Streptomyces venezuelae. , 2001, Journal of natural products.

[90]  R. McDaniel,et al.  Combinatorial biosynthesis of antimicrobials and other natural products. , 2001, Current opinion in microbiology.

[91]  Yoshiyuki Sakaki,et al.  Genome sequence of an industrial microorganism Streptomyces avermitilis: Deducing the ability of producing secondary metabolites , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[92]  R. Lill,et al.  New erythromycin derivatives from Saccharopolyspora erythraea using sugar O‐methyltransferases from the spinosyn biosynthetic gene cluster , 2001, Molecular microbiology.

[93]  R McDaniel,et al.  Construction of desosamine containing polyketide libraries using a glycosyltransferase with broad substrate specificity. , 2001, Chemistry & biology.

[94]  L. Katz,et al.  Biosynthesis of the antiparasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea , 2001 .

[95]  A. D. Buss,et al.  Engineering a polyketide with a longer chain by insertion of an extra module into the erythromycin-producing polyketide synthase. , 2001, Chemistry & biology.

[96]  P. Leadlay,et al.  Molecular basis of Celmer's rules: role of the ketosynthase domain in epimerisation and demonstration that ketoreductase domains can have altered product specificity with unnatural substrates. , 2001, Chemistry & biology.

[97]  B A Pfeifer,et al.  Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of E. coli , 2001, Science.

[98]  Blaine A. Pfeifer,et al.  Biosynthesis of Polyketides in Heterologous Hosts , 2001, Microbiology and Molecular Biology Reviews.

[99]  C. Khosla,et al.  Intermodular communication in polyketide synthases: comparing the role of protein-protein interactions to those in other multidomain proteins. , 2001, Biochemistry.

[100]  D. Cane,et al.  Selective protein-protein interactions direct channeling of intermediates between polyketide synthase modules. , 2001, Biochemistry.

[101]  J. Staunton,et al.  Polyketide biosynthesis: a millennium review. , 2001, Natural product reports.

[102]  L. Katz,et al.  Biosynthesis of the anti‐parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea , 2000, Molecular microbiology.

[103]  S. Shah,et al.  Cloning, characterization and heterologous expression of a polyketide synthase and P-450 oxidase involved in the biosynthesis of the antibiotic oleandomycin. , 2000, The Journal of antibiotics.

[104]  S. Gaisser,et al.  A defined system for hybrid macrolide biosynthesis in Saccharopolyspora erythraea , 2000, Molecular microbiology.

[105]  E. Bossi,et al.  Artificial chromosomes for antibiotic-producing actinomycetes , 2000, Nature Biotechnology.

[106]  R McDaniel,et al.  Formation of functional heterologous complexes using subunits from the picromycin, erythromycin and oleandomycin polyketide synthases. , 2000, Chemistry & biology.

[107]  C. Khosla,et al.  Cloning and heterologous expression of the epothilone gene cluster. , 2000, Science.

[108]  C R Hutchinson,et al.  A multiplasmid approach to preparing large libraries of polyketides. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[109]  P. Leadlay,et al.  Knowledge-based design of bimodular and trimodular polyketide synthases based on domain and module swaps: a route to simple statin analogues. , 1999, Chemistry & biology.

[110]  R. Mcdaniel,et al.  Elucidating the mechanism of chain termination switching in the picromycin/methymycin polyketide synthase. , 1999, Chemistry & biology.

[111]  D. Cane,et al.  Dissecting and exploiting intermodular communication in polyketide synthases. , 1999, Science.

[112]  R. Ziermann,et al.  Recombinant polyketide synthesis in Streptomyces: engineering of improved host strains. , 1999, BioTechniques.

[113]  D. Sherman,et al.  Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae. , 1998, Chemistry & biology.

[114]  C. Walsh,et al.  Harnessing the biosynthetic code: combinations, permutations, and mutations. , 1998, Science.

[115]  Frank Buchholz,et al.  A new logic for DNA engineering using recombination in Escherichia coli , 1998, Nature Genetics.

[116]  H. Floss,et al.  3-Amino-5-hydroxybenzoic Acid Synthase, the Terminal Enzyme in the Formation of the Precursor of mC7N Units in Rifamycin and Related Antibiotics* , 1998, The Journal of Biological Chemistry.

[117]  Camilla M. Kao,et al.  ALCOHOL STEREOCHEMISTRY IN POLYKETIDE BACKBONES IS CONTROLLED BY THE BETA -KETOREDUCTASE DOMAINS OF MODULAR POLYKETIDE SYNTHASES , 1998 .

[118]  C. Walsh,et al.  Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases. , 1998, Biochemistry.

[119]  Camilla M. Kao,et al.  GAIN OF FUNCTION MUTAGENESIS OF THE ERYTHROMYCIN POLYKETIDE SYNTHASE. 2. ENGINEERED BIOSYNTHESIS OF AN EIGHT-MEMBERED RING TETRAKETIDE LACTONE , 1997 .

[120]  P. Leadlay,et al.  The molecular basis of Celmer's rules: the stereochemistry of the condensation step in chain extension on the erythromycin polyketide synthase. , 1997, Biochemistry.

[121]  P. Leadlay,et al.  A hybrid modular polyketide synthase obtained by domain swapping. , 1996, Chemistry & biology.

[122]  C. Khosla,et al.  Generation of polyketide libraries via combinatorial biosynthesis. , 1996, Trends in biotechnology.

[123]  James Staunton,et al.  Evidence for a double-helical structure for modular polyketide synthases , 1996, Nature Structural Biology.

[124]  M. Marahiel,et al.  A new enzyme superfamily - the phosphopantetheinyl transferases. , 1996, Chemistry & biology.

[125]  P. Leadlay,et al.  Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl‐CoA:acyl carrier protein transacylase domains in modular polyketide synthases , 1995, FEBS letters.

[126]  Camilla M. Kao,et al.  Manipulation of macrolide ring size by directed mutagenesis of a modular polyketide synthase , 1995 .

[127]  P. Leadlay,et al.  Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage. , 1995, Science.

[128]  K. Schleifer,et al.  Phylogenetic identification and in situ detection of individual microbial cells without cultivation. , 1995, Microbiological reviews.

[129]  P. Leadlay,et al.  The thioesterase of the erythromycin-producing polyketide synthase: mechanistic studies in vitro to investigate its mode of action and substrate specificity , 1995 .

[130]  C M Kao,et al.  Engineered biosynthesis of a complete macrolactone in a heterologous host. , 1994, Science.

[131]  P. Leadlay,et al.  Stereospecific acyl transfers on the erythromycin-producing polyketide synthase. , 1994, Science.

[132]  M. Ryan,et al.  Benefit to male sailfin mollies of mating with heterospecific females. , 1994, Science.

[133]  P. Leadlay,et al.  Heterologous expression in Escherichia coli of an intact multienzyme component of the erythromycin-producing polyketide synthase. , 1993, European journal of biochemistry.

[134]  C. Khosla,et al.  Engineered biosynthesis of novel polyketides. , 1993, Science.

[135]  J B McAlpine,et al.  Modular organization of genes required for complex polyketide biosynthesis. , 1991, Science.

[136]  P. Leadlay,et al.  An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea , 1990, Nature.

[137]  P. Bartel,et al.  Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: clarification of actinorhodin gene functions , 1990, Journal of Bacteriology.

[138]  D. Hopwood,et al.  Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host , 1984, Nature.