Airborne gram-negative bacteria and endotoxin in sick building syndrome. A study in Dutch governmental office buildings.

METHODS During a survey of building-related symptoms among 1355 employees working in 19 governmental office buildings in the Netherlands, we measured physical and chemical factors, airborne microorganisms, and endotoxin. Mechanically ventilated buildings were grouped as "healthy" or "sick" based on symptom prevalence (mean symptom prevalence < 15% or > or = 15%). We compared physical, chemical, and microbiological characteristics between mechanically ventilated and naturally ventilated buildings. RESULTS Gram-negative rods were found in higher numbers in the "sick" buildings. The concentration of airborne endotoxin was six to seven times higher in the "sick" buildings than in the "healthy" buildings (254 vs 46 ng/m3); it was 35 ng/m3 in naturally ventilated buildings. No differences in physical characteristics of the buildings were found. Volatile organic compounds and carbon dioxide were present at higher concentrations in naturally ventilated buildings. CONCLUSION Airborne microbial contamination, in particular with gram-negative rods and perhaps with endotoxin, may have a role in the causation of sick building syndrome.

[1]  Spendlove Jc,et al.  Source, significance, and control of indoor microbial aerosols: human health aspects. , 1983 .

[2]  K Kreiss.  The sick building syndrome in office buildings--a breath of fresh air. , 1993, The New England journal of medicine.

[3]  R. McGee,et al.  Airborne microbial contaminants in indoor environments. Naturally ventilated and air-conditioned homes. , 1986, Archives of environmental health.

[4]  C A Pickering,et al.  Building related illness , 1986, Clinical allergy.

[5]  E. Faragher,et al.  An investigation of the relationship between microbial and particulate indoor air pollution and the sick building syndrome. , 1992, Respiratory medicine.

[6]  R. Rylander,et al.  Airborne endotoxin in industries processing vegetable fibers. , 1982, American Industrial Hygiene Association journal.

[7]  L. Brosseau,et al.  Comparison of Sampling Media for Endotoxin-Contaminated Aerosols , 1992 .

[8]  R Rylander,et al.  Environmental and health studies of farm workers in Swedish swine confinement buildings. , 1989, British journal of industrial medicine.

[9]  P. Skov,et al.  Influence of indoor climate on the sick building syndrome in an office environment. The Danish Indoor Climate Study Group. , 1990, Scandinavian journal of work, environment & health.

[10]  J. Edwards,et al.  Microbial and immunological investigations and remedial action after an outbreak of humidifier fever , 1980, British journal of industrial medicine.

[11]  K O Gundermann,et al.  SPREAD OF MICROORGANISMS BY AIR‐CONDITIONING SYSTEMS—ESPECIALLY IN HOSPITALS , 1980, Annals of the New York Academy of Sciences.

[12]  Epilogue: Health effects of organic dusts in the farm environment , 1986 .

[13]  R. Rylander.  Plant constituents of cotton dust and lung effects after inhalation. , 1988, The European respiratory journal.

[14]  D. Heederik,et al.  Relationship of airborne endotoxin and bacteria levels in pig farms with the lung function and respiratory symptoms of farmers , 1991, International archives of occupational and environmental health.

[15]  R Menzies,et al.  The effect of varying levels of outdoor-air supply on the symptoms of sick building syndrome. , 1993, The New England journal of medicine.

[16]  D. Norbäck,et al.  Environmental, occupational, and personal factors related to the prevalence of sick building syndrome in the general population. , 1991, British journal of industrial medicine.

[17]  H. Burger.  Bioaerosols: prevalence and health effects in the indoor environment. , 1990 .

[18]  M J Hodgson,et al.  Symptoms and microenvironmental measures in nonproblem buildings. , 1991, Journal of occupational medicine. : official publication of the Industrial Medical Association.

[19]  D. Flaherty,et al.  Bacterial endotoxin isolated from a water spray air humidification system as a putative agent of occupation-related lung disease , 1984, Infection and immunity.

[20]  A. Smith,et al.  Consistent pattern of elevated symptoms in air-conditioned office buildings: a reanalysis of epidemiologic studies. , 1990, American journal of public health.

[21]  A Hedge,et al.  Comparison of health problems related to work and environmental measurements in two office buildings with different ventilation systems. , 1985, British medical journal.

[22]  J. Fink,et al.  Hypersensitivity pneumonitis due to contamination of an air conditioner. , 1970, The New England journal of medicine.

[23]  Report of Diseases , 1819, The London medical and physical journal.

[24]  R. Rylander,et al.  Humidifier fever and endotoxin exposure , 1978, Clinical allergy.

[25]  O. Valbjørn,et al.  The “sick” building syndrome in the office environment: The Danish town hall study , 1987 .

[26]  E J Reed,et al.  Biological impact of small air ions. , 1976, Science.

[27]  J L Hankinson,et al.  Inhaled endotoxin and decreased spirometric values. An exposure-response relation for cotton dust. , 1987, The New England journal of medicine.

[28]  R. A. Young,et al.  The effects of reduced ventilation on indoor air quality in an office building , 1983 .

[29]  R. Rylander,et al.  Endotoxin in cotton dust and respiratory function decrement among cotton workers in an experimental cardroom. , 1985, The American review of respiratory disease.

[30]  A. J. Streifel,et al.  ELEVATED AIRBORNE CONCENTRATIONS OF FUNGI IN RESIDENTIAL AND OFFICE ENVIRONMENTS , 1990 .

[31]  A Hedge,et al.  Sick building syndrome: a study of 4373 office workers. , 1987, The Annals of occupational hygiene.

[32]  J.S.M. Boleij,et al.  Gezondheidsklachten en klachten over het binnenklimaat in kantoorgebouwen. , 1990 .

[33]  C A Pickering,et al.  The sick building syndrome: prevalence studies. , 1984, British medical journal.

[34]  A. Hedge,et al.  Air ionization: an evaluation of its physiological and psychological effects. , 1982, The Annals of occupational hygiene.