Protease detection using a porous silicon based Bloch surface wave optical biosensor.

In this article we present an optical biosensor for label-free detection of trace levels of protease activity. The scheme is based on surface functionalized porous silicon optical structures which supports optical Bloch surface modes. The optical structure provides a resonant optical mode for high sensitivity detection and open access of the sensing layer to the target enzyme. Protease detection is based on the digestion of gelatin, covalently attached inside the pore space, resulting in a spectral blue-shift of the optical mode. Monitoring of spatially separated resonant optical modes is used to eliminate optical response from nonspecific adsorption.

[1]  Kristopher A Kilian,et al.  Peptide-modified optical filters for detecting protease activity. , 2007, ACS nano.

[2]  Katharina Gaus,et al.  Porous silicon based narrow line-width rugate filters , 2007 .

[3]  Michael J Sailor,et al.  The smart Petri dish: a nanostructured photonic crystal for real-time monitoring of living cells. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[4]  V. A. Tolmachev,et al.  Effective refractive index and composition of oxidized porous silicon films , 2000 .

[5]  T. Benyattou,et al.  Surface wave photonic device based on porous silicon multilayers , 2006 .

[6]  W. Robertson,et al.  Surface plasmon-like sensor based on surface electromagnetic waves in a photonic band-gap material , 2005 .

[7]  T. Benyattou,et al.  Bragg surface wave device based on porous silicon and its application for sensing , 2007 .

[8]  M. Gal,et al.  Broadband laser mirrors made from porous silicon , 2004 .

[9]  Francesco Michelotti,et al.  Coupling of surface waves in highly defined one-dimensional porous silicon photonic crystals for gas sensing applications , 2007 .

[10]  L. Dominici,et al.  Experimental determination of the sensitivity of Bloch surface waves based sensors. , 2010, Optics express.

[11]  Philippe M. Fauchet,et al.  Quantitative analysis of the sensitivity of porous silicon optical biosensors , 2006 .

[12]  Gilles Lerondel,et al.  Optical microcavities with subnanometer linewidths based on porous silicon , 2002 .

[13]  Kristopher A Kilian,et al.  The importance of surface chemistry in mesoporous materials: lessons from porous silicon biosensors. , 2009, Chemical communications.

[14]  Kristopher A Kilian,et al.  Smart tissue culture: in situ monitoring of the activity of protease enzymes secreted from live cells using nanostructured photonic crystals. , 2009, Nano letters.

[15]  Leigh T. Canham,et al.  Lewis Acid Mediated Hydrosilylation on Porous Silicon Surfaces , 1999 .

[16]  J. Kärger,et al.  Characterization of pore size distribution in porous silicon by NMR cryoporosimetry and adsorption methods , 2008 .

[17]  Christopher L. Brown,et al.  Immobilization of dendrimers on Si–C linked carboxylic acid-terminated monolayers on silicon(111) , 2006 .

[18]  M. Ghadiri,et al.  A porous silicon-based optical interferometric biosensor. , 1997, Science.

[19]  Huimin Ouyang,et al.  Label-free quantitative detection of protein using macroporous silicon photonic bandgap biosensors. , 2007, Analytical chemistry.

[20]  Katharina Gaus,et al.  Modifying Porous Silicon with Self‐Assembled Monolayers for Biomedical Applications: The Influence of Surface Coverage on Stability and Biomolecule Coupling , 2008 .