Quantum Clusters of Gold Exhibiting FRET

Fluorescence resonance energy transfer between the metal core and the ligand in Au 25 SG 18 (SG-glutathione thiolate) is demonstrated using dansyl chromophores attached to the cluster core through glutathione linkers. The dansyl chromophore functionalization of the cluster has been carried out by two different routes. Efficient energy transfer from the dansyl donor to the Au 25 core is manifested by way of the reduced lifetime of the excited state of the former and drastic quenching of its fluorescence. The donor-acceptor separation observed in the two synthetic routes reflects the asymmetry in the ligand binding on the cluster core, which is in agreement with the recent theoretical and experimental results on the structure of Au 25 .

[1]  R. Jin,et al.  Correlating the crystal structure of a thiol-protected Au25 cluster and optical properties. , 2008, Journal of the American Chemical Society.

[2]  R. Whetten,et al.  On the structure of thiolate-protected Au25. , 2008, Journal of the American Chemical Society.

[3]  R. Murray,et al.  Crystal structure of the gold nanoparticle [N(C8H17)4][Au25(SCH2CH2Ph)18]. , 2008, Journal of the American Chemical Society.

[4]  R. Jin,et al.  Kinetically controlled, high-yield synthesis of Au25 clusters. , 2008, Journal of the American Chemical Society.

[5]  T. Pradeep,et al.  Reactivity of Au25 clusters with Au3 , 2007 .

[6]  Paresh Chandra Ray,et al.  Gold nanoparticle-based miniaturized nanomaterial surface energy transfer probe for rapid and ultrasensitive detection of mercury in soil, water, and fish. , 2007, ACS nano.

[7]  S. Pal,et al.  Direct conjugation of semiconductor nanocrystals to a globular protein to study protein-folding intermediates. , 2007, The journal of physical chemistry. B.

[8]  J. Watanabe,et al.  Instantaneous determination via bimolecular recognition: usefulness of FRET in phosphorylcholine group enriched nanoparticles. , 2007, Bioconjugate chemistry.

[9]  Robert Langer,et al.  Quantum dot-aptamer conjugates for synchronous cancer imaging, therapy, and sensing of drug delivery based on bi-fluorescence resonance energy transfer. , 2007, Nano letters.

[10]  Genhua Wu,et al.  A functionalized gold nanoparticles and Rhodamine 6G based fluorescent sensor for high sensitive and selective detection of mercury(II) in environmental water samples. , 2007, Analytica chimica acta.

[11]  G. Darbha,et al.  Gold Nanoparticle Based FRET for DNA Detection , 2007 .

[12]  Y. Negishi,et al.  Extremely high stability of glutathionate-protected Au25 clusters against core etching. , 2007, Small.

[13]  Robert M Dickson,et al.  Highly fluorescent noble-metal quantum dots. , 2007, Annual review of physical chemistry.

[14]  Chan Joong Kim,et al.  Noninvasive probing of the spatial organization of polymer chains in hydrogels using fluorescence resonance energy transfer (FRET). , 2007, Journal of the American Chemical Society.

[15]  L. Prodi,et al.  Enhanced sensitized NIR luminescence from gold nanoparticles via energy transfer from surface-bound fluorophores. , 2007, Journal of the American Chemical Society.

[16]  J. K. Hurst,et al.  Reversibly photoswitchable dual-color fluorescent nanoparticles as new tools for live-cell imaging. , 2007, Journal of the American Chemical Society.

[17]  M. V. D. van de Corput,et al.  Nanoparticle-induced fluorescence lifetime modification as nanoscopic ruler: demonstration at the single molecule level. , 2007, Nano letters.

[18]  S. Nie,et al.  Etching colloidal gold nanocrystals with hyperbranched and multivalent polymers: a new route to fluorescent and water-soluble atomic clusters. , 2007, Journal of the American Chemical Society.

[19]  J. Lakowicz,et al.  Enhanced Förster Resonance Energy Transfer (FRET) on Single Metal Particle. , 2007, The journal of physical chemistry. C, Nanomaterials and interfaces.

[20]  Samir Kumar Pal,et al.  Aggregated CdS quantum dots: Host of biomolecular ligands. , 2006, The journal of physical chemistry. B.

[21]  Paresh Chandra Ray,et al.  Gold nanoparticle based FRET assay for the detection of DNA cleavage. , 2006, The journal of physical chemistry. B.

[22]  C. Ahn,et al.  Synthesis of a PEGylated polymeric pH sensor and its pH sensitivity by fluorescence resonance energy transfer , 2006 .

[23]  Zeev Rosenzweig,et al.  Synthesis and application of quantum dots FRET-based protease sensors. , 2006, Journal of the American Chemical Society.

[24]  M. Singh,et al.  Fluorescent lifetime quenching near d = 1.5 nm gold nanoparticles: probing NSET validity. , 2006, Journal of the American Chemical Society.

[25]  Qiang Ma,et al.  Fluorescence resonance energy transfer in doubly-quantum dot labeled IgG system. , 2005, Talanta.

[26]  Y. Negishi,et al.  Large-scale synthesis of thiolated Au25 clusters via ligand exchange reactions of phosphine-stabilized Au11 clusters. , 2005, Journal of the American Chemical Society.

[27]  Jinping Gan,et al.  Dansyl glutathione as a trapping agent for the quantitative estimation and identification of reactive metabolites. , 2005, Chemical research in toxicology.

[28]  Katsuyuki Nobusada,et al.  Glutathione-protected gold clusters revisited: bridging the gap between gold(I)-thiolate complexes and thiolate-protected gold nanocrystals. , 2005, Journal of the American Chemical Society.

[29]  Eunkeu Oh,et al.  Inhibition assay of biomolecules based on fluorescence resonance energy transfer (FRET) between quantum dots and gold nanoparticles. , 2005, Journal of the American Chemical Society.

[30]  Johannes Buchner,et al.  Protein folding handbook , 2005 .

[31]  Huixiang Li,et al.  DNA sequence detection using selective fluorescence quenching of tagged oligonucleotide probes by gold nanoparticles. , 2004, Analytical chemistry.

[32]  R. Dickson,et al.  Highly fluorescent, water-soluble, size-tunable gold quantum dots. , 2004, Physical review letters.

[33]  H. Weller,et al.  Investigation of Energy Transfer between CdTe Nanocrystals on Polystyrene Beads and Dye Molecules for FRET-SNOM Applications† , 2004 .

[34]  Huan‐Tsung Chang,et al.  Nile red-adsorbed gold nanoparticles for selective determination of thiols based on energy transfer and aggregation. , 2004, Analytical chemistry.

[35]  Zhiyong Tang,et al.  Simple Preparation Strategy and One-Dimensional Energy Transfer in CdTe Nanoparticle Chains , 2004 .

[36]  Igor Nabiev,et al.  Energy Transfer in Aqueous Solutions of Oppositely Charged CdSe/ZnS Core/Shell Quantum Dots and in Quantum Dot−Nanogold Assemblies , 2004 .

[37]  R. Dickson,et al.  High quantum yield blue emission from water-soluble Au8 nanodots. , 2003, Journal of the American Chemical Society.

[38]  A. Zewail,et al.  Femtosecond studies of protein-DNA binding and dynamics: histone I. , 2001, Chemphyschem : a European journal of chemical physics and physical chemistry.

[39]  Robert L. Whetten,et al.  Isolation and Selected Properties of a 10.4 kDa Gold:Glutathione Cluster Compound , 1998 .

[40]  T. Thirunamachandran,et al.  Molecular Quantum Electrodynamics , 1984 .

[41]  B. Persson,et al.  Electron-hole-pair quenching of excited states near a metal , 1982 .

[42]  W. Melhuish,et al.  Measurement of Quantum Efficiencies of Fluorescence and Phosphorescence and Some Suggested Luminescence Standards , 1964 .

[43]  N. Rosenzweig,et al.  Luminescent quantum dots fluorescence resonance energy transfer-based probes for enzymatic activity and enzyme inhibitors. , 2007, Analytical chemistry.

[44]  Uri Banin,et al.  Quantum-Dot-Functionalized Scanning Probes for Fluorescence-Energy-Transfer-Based Microscopy , 2004 .

[45]  J. Lakowicz.  Principles of fluorescence spectroscopy , 1983 .