A model for the computer analysis of synchronous DNA distributions obtained by flow cytometry.

A new model for obtaining the distributions of cells in the cell cycle by computer analysis of flow cytometry data is proposed. The model is able to analyze distributions from both asynchronous and synchronous cell populations. A culture of HeLa cells is pulse-labeled with tritiated thymidine at various times after synchronization by the mitotic selection method, then analyzed by flow cytometry and autoradiography. The results show good agreement between the percentage S-phase cells determined by the model and by autoradiography.

[1]  D. Bloch,et al.  Chapter 14 Flow-Cytometric Analysis of Chromatin , 1978 .

[2]  B. Barlogie,et al.  Pulse cytophotometric analysis of synchronized cells in vitro. , 1976, Cancer research.

[3]  J. Jett,et al.  MATHEMATICAL ANALYSIS OF DNA DISTRIBUTIONS DERIVED FROM FLOW MICROFLUOROMETRY , 1974, The Journal of cell biology.

[4]  Enhanced light collection in a flow cytometer. , 1980, Cytometry.

[5]  T. Lindmo,et al.  Selection of optimal model for the DNA histogram by analysis of error of estimated parameters. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[6]  L. Tolmach,et al.  Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. , 1963, Experimental cell research.

[7]  W. Göhde,et al.  Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle , 1975, Radiation and environmental biophysics.

[8]  R. G. Ham,et al.  An improved nutrient solution for diploid Chinese hamster and human cell lines. , 1963, Experimental cell research.

[9]  R. Jensen.  Chromomycin A3 as a fluorescent probe for flow cytometry of human gynecologic samples. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  J. Fried.  Method for the quantitative evaluation of data from flow microfluorometry. , 1976, Computers and biomedical research, an international journal.

[11]  P. F. Mullaney,et al.  Cell Microfluorometry: A Method for Rapid Fluorescence Measurement , 1969, Science.

[12]  M. Kim,et al.  Mathematical methods for determining cell DNA synthesis rate and age distribution utilizing flow microfluorometry. , 1977, Journal of theoretical biology.

[13]  J. Gray,et al.  CELL CYCLE ANALYSIS FROM COMPUTER SYNTHESIS OF DEOXYRIBONUCLEIC ACID HISTOGRAMS , 1974, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[14]  P. R. Bevington,et al.  Data Reduction and Error Analysis for the Physical Sciences , 1969 .

[15]  J. Gray.  CELL‐CYCLE ANALYSIS OF PERTURBED CELL POPULATIONS: COMPUTER SIMULATION OF SEQUENTIAL DNA DISTRIBUTIONS , 1976, Cell and tissue kinetics.

[16]  K. Woo.  THE DISCRETE–TIME KINETIC MODEL ANALYSIS OF DNA CONTENT DISTRIBUTIONS IN EXPERIMENTAL TUMOUR CELLS , 1979, Cell and tissue kinetics.

[17]  J. Fried,et al.  Quantitative analysis of flow microfluorometric data from asynchronous and drug-treated cell populations. , 1976, Computers and biomedical research, an international journal.

[18]  L. S. Cram,et al.  Laser Flow Microphotometers for Rapid Analysis and Sorting of Individual Mammalian Cells , 1974 .

[19]  D. Marquardt.  An Algorithm for Least-Squares Estimation of Nonlinear Parameters , 1963 .

[20]  J. Fried.  Analysis of deoxyribonucleic acid histograms from flow cytofluorometry. Estimation of the distribution of cells within S phase. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[21]  L. Wheeless,et al.  Quantitative single cell analysis and sorting. , 1977, Science.