Covalent electropolymerization of glucose oxidase in polypyrrole

Glucose oxidase (GOx) has been chemically derivatized by coupling N-(2-carboxyethyl)pyrrole to surface lysyl residues with a carbodiimide-promoted reaction. The modified enzyme (30.2±0.5 mol of pyrrole/mol of GOx) displayed almost identical properties to the native enzyme except that the pI was slightly more acidic and the stability was 6-fold higher at pH 7.0 and 60 o C. The protein-substituted pyrroles were accessible to electrochemical oxidation and in the presence of free pyrrole generated copolymers which covalently incorporated GOx

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