Regulation of substance P mRNA expression in human dermal microvascular endothelial cells.

Vascular endothelial cells have been identified as a source of substance P (SP) which may act in an autocrine/paracrine fashion to bring about nitric oxide (NO)-dependent vasodilatation and mitogen-induced cell division or immunologic and inflammatory responses. Whilst SP is localised in and released from endothelial cells, an endothelial mRNA expression of SP has not previously been shown. In the present study, mRNA expression of SP in human dermal microvascular endothelial cells is demonstrated using in situ hybridisation techniques with enhancement procedures. Incubation of microvascular endothelial cells with nerve growth factor (NGF) under conditions of increased shear stress increases the mRNA expression and release of SP Endothelin (ET) release is also enhanced. These changes are pertinent to circulatory, events that may occur in Raynaud's phenomenon in systemic sclerosis.

[1]  M. Matucci Cerinic,et al.  Beauty and the beast. The nitric oxide paradox in systemic sclerosis. , 2002, Rheumatology.

[2]  R. Caldwell,et al.  Vascular and endothelial actions of inhibitors of substance P amidation. , 2000, Journal of cardiovascular pharmacology.

[3]  G. Burnstock,et al.  Increased release of ATP from endothelial cells during acute inflammation , 1998, Inflammation Research.

[4]  G. Burnstock,et al.  Chronic sensory denervation reduces thrombin-stimulated endothelin release from aortic endothelial cells , 1996, Experientia.

[5]  G. Burnstock,et al.  Synergistic effect of acute hypoxia on flow-induced release of ATP from cultured endothelial cells , 1995, Experientia.

[6]  C. Murphy,et al.  Growth-promoting effects of substance P on endothelial cells in vitro. Synergism with calcitonin gene-related peptide, insulin, and plasma factors. , 1994, Circulation research.

[7]  T. Hidaka,et al.  Increased endothelin-1 production in fibroblasts derived from patients with systemic sclerosis. , 1994, Annals of the rheumatic diseases.

[8]  M. Matucci-Cerinic.  Sensory neuropeptides and rheumatic diseases. , 1993, Rheumatic diseases clinics of North America.

[9]  C. Bunker,et al.  Image analysis quantification of peptide‐immunoreactive nerves in the skin of patients with Raynaud's phenomenon and systemic sclerosis , 1991, The Journal of pathology.

[10]  C. Bunker,et al.  Deficiency of calcitonin gene-related peptide in Raynaud's phenomenon , 1990, The Lancet.

[11]  G. Burnstock,et al.  Endothelial cells cultured from human umbilical vein release ATP, substance P and acetylcholine in response to increased flow , 1990, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[12]  G. Burnstock,et al.  Substance P is released from the endothelium of normal and capsaicin-treated rat hind-limb vasculature, in vivo, by increased flow. , 1990, Circulation research.

[13]  M. Moskowitz,et al.  Identification of immunoreactive substance P in human and other mammalian endothelial cells , 1989, Peptides.

[14]  M. Shichiri,et al.  Concomitant secretion of big endothelin and its C-terminal fragment from human and bovine endothelial cells. , 1989, Biochemical and biophysical research communications.

[15]  G. Burnstock,et al.  Ultrastructural localisation of substance P and choline acetyltransferase in endothelial cells of rat coronary artery and release of substance P and acetylcholine during hypoxia , 1989, Experientia.

[16]  P. M. Campbell,et al.  Pathogenesis of systemic sclerosis: a vascular hypothesis. , 1975, Seminars in arthritis and rheumatism.

[17]  G. Burnstock,et al.  Ultrastructural localisation of serotonin and substance P in vascular endothelial cells of rat femoral and mesenteric arteries , 2006, Anatomy and Embryology.

[18]  G. Burnstock,et al.  Modulation of Endothelin Release by Vasoactive Peptides Localised in Human Umbilical Vein Endothelial Cells , 1996 .

[19]  M. Cerinic,et al.  Raynaud's phenomenon and scleroderma. Dysregulated neuroendothelial control of vascular tone. , 1995, Arthritis and rheumatism.

[20]  G. Burnstock,et al.  Interactions between sensory perivascular nerves and the endothelium in brain microvessels. , 1995, International journal of microcirculation, clinical and experimental.